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Plant Molecular Biology Reporter

, Volume 34, Issue 1, pp 1–14 | Cite as

CarHSFB2, a Class B Heat Shock Transcription Factor, Is Involved in Different Developmental Processes and Various Stress Responses in Chickpea (Cicer Arietinum L.)

  • Hao MaEmail author
  • Chuntao Wang
  • Bin Yang
  • Huiying Cheng
  • Ze Wang
  • Abudoukeyumu Mijiti
  • Cai Ren
  • Guanghang Qu
  • Hua Zhang
  • Lin Ma
Original Paper

Abstract

Heat shock transcription factor (HSF) plays an essential role on the increased tolerance against heat stress by regulating the expression of heat-responsive genes. In this study, a HSF gene, CarHSFB2, was isolated and characterized in chickpea. CarHSFB2 was a nuclear protein with a predicted polypeptide of 267 amino acids and encoded by a single/low copy genes. Phylogenetic analysis showed that CarHSFB2 belonged to the class B HSFs. It had little or no any transcription activation activity due to lack of aromatic, hydrophobic, and acidic amino acid (AHA) motifs. CarHSFB2 showed different expression patterns among different developmental processes (leaf senescence, developing seed, and embryo of germinating seed). It was induced by the stress of heat, salt, wound and drought, and the treatment of H2O2, IAA, and GA3, respectively, while inhibited by 6-BA. However, the other stress and chemical treatments (cold, ABA, MeJA, Et, and SA) had no obvious effect on its expression. Overexpression of CarHSFB2 in Arabidopsis seedlings showed the increased tolerance to drought and heat stress. Additionally, stress-responsive genes, RD22, RD26, and RD29A, showed significantly higher expression levels in transgenic Arabidopsis seedlings than in the wild type (WT) under drought stress, whereas HsfA2, HsfB2a, and HsfA7a in transgenic Arabidopsis seedlings were markedly accumulated in transcript level than in the WT under heat stress. All these results indicate that CarHSFB2, a class B HSF, positively functions in different developmental processes and various stress responses, especially in positive response to heat and drought stresses, in chickpea.

Keywords

Chickpea Heat shock transcription factor Developmental processes Heat stress Drought stress 

Abbreviations

6-BA

N-6-benzyl-adenine

ABA

Abscisic acid

AHA

Aromatic, hydrophobic, and acidic amino acids

CTD

C-terminal domain

CTR

C-terminal region

DAF

Days after flowering

DBD

DNA-binding domain

Et

Ethephon

EST

Expressed sequence tag

GA3

Gibberellin

GSP

Gene-specific primer

IAA

Indole-3-acetic acid

HAI

Hours after the initial imbibition

HR-A/B

Heptad pattern of hydrophobic residues

HSE

Heat stress element

HSP

Heat shock protein

HSF

Heat shock transcription factor

Lp

Lycopersicon peruvianum

MeJA

Methyl jasmonate

NGSP

Gene-specific primer for the nested PCR

NLS

Nuclear localization signal

ORF

Open reading frame

Os

Oryza sativa

RACE

Rapid amplification of cDNA end

RT-PCR

Reverse transcription-polymerase chain reaction

SA

Salicylic acid

UTR

Untranslated region

WT

Wild type

Notes

Acknowledgments

We gratefully acknowledge the partial financial support from the projects supported by the National Natural Science Foundation of China (31160306, 30960201, 30860152, and 31160300) and the Xinjiang Science and Technology Department of China (200991254) for this research.

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Hao Ma
    • 1
    • 2
    Email author
  • Chuntao Wang
    • 1
  • Bin Yang
    • 1
  • Huiying Cheng
    • 1
  • Ze Wang
    • 2
  • Abudoukeyumu Mijiti
    • 2
  • Cai Ren
    • 1
  • Guanghang Qu
    • 1
  • Hua Zhang
    • 2
  • Lin Ma
    • 2
  1. 1.State Key Laboratory of Crop Genetics and Germplasm EnhancementNanjing Agricultural UniversityNanjingChina
  2. 2.Desert Research Institute in the Arid Region|Xinjiang Agricultural UniversityUrumqiChina

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