Plant Molecular Biology Reporter

, Volume 33, Issue 4, pp 1107–1115 | Cite as

Tobacco Chloroplasts as Bioreactors for the Production of Recombinant Superoxide Dismutase in Plants, an Industrially Useful Enzyme

  • Raju Madanala
  • Vijayta Gupta
  • Ashutosh Kumar Pandey
  • Subhi Srivastava
  • Vivek Pandey
  • Pradhyumna Kumar Singh
  • Rakesh Tuli
Original Paper


An inexpensive source of industrially useful enzymes is critical for their commercial production. We have produced an industrially valuable recombinant superoxide dismutase (SOD) in tobacco chloroplasts. A gene from Withania somnifera, encoding a highly stable Cu/Zn SOD, was cloned into a chloroplast transformation vector. It expressed the SOD in tobacco chloroplasts following transformation. The transplastomic plants accumulated the recombinant SOD at up to ∼9 % of the total soluble protein in leaves. The purified chloroplast-expressed recombinant SOD had an estimated specific activity of ∼4600 U/mg. Like the native enzyme, purified recombinant enzyme, prepared from tobacco leaves, was highly stable at high temperatures and tolerated a wide pH range, SDS, ethanol and protease treatment. The results establish the potential of chloroplast transformation for commercial production of recombinant SOD in plants.


Chloroplast transformation Cu/Zn SOD Recombinant proteins Transplastomic plants Tobacco 

Supplementary material

11105_2014_805_MOESM1_ESM.doc (404 kb)
ESM 1(DOC 404 kb)


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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Raju Madanala
    • 1
  • Vijayta Gupta
    • 2
  • Ashutosh Kumar Pandey
    • 1
  • Subhi Srivastava
    • 1
  • Vivek Pandey
    • 1
  • Pradhyumna Kumar Singh
    • 1
  • Rakesh Tuli
    • 3
  1. 1.National Botanical Research InstituteCouncil of Scientific and Industrial Research (CSIR)LucknowIndia
  2. 2.Central Institute of Medicinal and Aromatic PlantsCouncil of Scientific and Industrial ResearchLucknowIndia
  3. 3.Department of BiotechnologyNational Agri-Food Biotechnology InstituteMohaliIndia

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