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Plant Molecular Biology Reporter

, Volume 33, Issue 4, pp 797–805 | Cite as

Expression and Functional Analysis of a Pathogenesis-Related Protein 1 Gene, TcLr19PR1, Involved in Wheat Resistance Against Leaf Rust Fungus

  • Lin Gao
  • Shen Wang
  • Xiao-Ying Li
  • Xue-Jun Wei
  • Yan-Jun Zhang
  • Hai-Yan WangEmail author
  • Da-Qun LiuEmail author
Original Paper

Abstract

Pathogenesis-related (PR) proteins encoded by plant defense genes play key roles in plant disease-resistance responses, specialized in systemic-acquired resistance. However, their roles in the response of wheat to fungal infection are still not well known. Our earlier studies have reported that a full-length TcLr19PR1 gene (818 bp) was isolated from wheat infected by leaf rust (Puccinia triticina). Here, we showed that TcLr19PR1 was induced earlier and its expression level was higher in the incompatible interaction between seedling stage of wheat and P. triticina than in compatible interactions. TcLr19PR1 was strongly induced after P. triticina inoculation and ABA and SA treatments, in which the expression level of TcLr19PR1 significantly increased and reached the maximum at 12 and 72 h, respectively. Furthermore, the transgenic T1-stable TcLr19PR1 lines generated in the susceptible wheat cultivar Zhengzhou 5389 background exhibited certain extent disease resistance against P. triticina infection by preventing disease development. In addition, Western blotting was conducted to confirm that TcLr19PR1 protein was induced by P. triticina infection in positive transgenic plants at the protein expression level. These findings suggest that TcLr19PR1 gene plays an important role in wheat development and resistance to leaf rust pathogen attack.

Keywords

TcLr19PR1 Real-time quantitative RT-PCR Transcripts expression profiles Gene gun transformation 

Abbreviations

PR

Pathogenesis related

HR

Hypersensitive response

ABA

Abscisic acid

SA

Salicylic acid

SAR

Systemic acquired resistance

ET

Ethylene

Hpi

Hours postinoculation

qRT-PCR

Quantitative reverse transcription-polymerase chain reaction

GAPDH

Glyceraldehyde-3-phosphate dehydrogenase

Notes

Acknowledgments

This study was supported financially by the Natural Science Foundation of Hebei Provice (no. C2012204005) and National key Basic Research Program of China (no. 2013CB127700).

Supplementary material

11105_2014_790_MOESM1_ESM.doc (238 kb)
ESM 1 (DOC 237 kb)

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  1. 1.Center of Plant Disease and Plant Pests of Hebei Province, College of Plant ProtectionAgricultural University of HebeiBaodingChina

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