Plant Molecular Biology Reporter

, Volume 33, Issue 3, pp 474–479 | Cite as

Identification and Validation of a SNP Marker Linked to the Gene HsBvm-1 for Nematode Resistance in Sugar Beet

  • Piergiorgio Stevanato
  • Daniele Trebbi
  • Lee Panella
  • Kelley Richardson
  • Chiara Broccanello
  • Linda Pakish
  • Ann L. Fenwick
  • Massimo Saccomani
Original Paper


The beet-cyst nematode (Heterodera schachtii Schmidt) is one of the major pests of sugar beet. The identification of molecular markers associated with nematode tolerance would be helpful for developing tolerant varieties. The aim of this study was to identify single nucleotide polymorphism (SNP) markers linked to nematode tolerance from the Beta vulgaris ssp. maritima source WB242. A WB242-derived F2 population was phenotyped for host-plant nematode reaction revealing a 3:1 segregation ratio of the tolerant and susceptible phenotypes and suggesting the action of a gene designated as HsBvm-1. Bulked segregant analysis (BSA) was used. The most tolerant and susceptible individuals were pooled and subjected to restriction site associated DNA sequencing (RAD-Seq) analysis, which identified 7,241 SNPs. A subset of 384 candidate SNPs segregating between bulks were genotyped on the 20 most-tolerant and most-susceptible individuals, identifying a single marker (SNP192) showing complete association with nematode tolerance. Segregation of SNP192 confirmed the inheritance of tolerance by a single gene. This association was further validated on a set of 26 commercial tolerant and susceptible varieties, showing the presence of the SNP192 WB242-type allele only in the tolerant varieties. We identified and mapped on chromosome 5 the first nematode tolerance gene (HsBvm-1) from Beta vulgaris ssp. maritima and released information on SNP192, a linked marker valuable for high-throughput, marker-assisted breeding of nematode tolerance in sugar beet.


HsBvm-1 Beta vulgaris ssp. maritima Biotic stresses Beet-cyst nematode WB242 genetic tolerance SNP 



Single nucleotide polymorphisms


Restriction site associated DNA sequencing


Bulk segregant analysis

Supplementary material

11105_2014_763_MOESM1_ESM.doc (29 kb)
Supplementary material S1(DOC 29 kb)
11105_2014_763_MOESM2_ESM.doc (28 kb)
Supplementary material S2(DOC 27 kb)


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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Piergiorgio Stevanato
    • 1
  • Daniele Trebbi
    • 1
  • Lee Panella
    • 2
  • Kelley Richardson
    • 3
  • Chiara Broccanello
    • 1
  • Linda Pakish
    • 3
  • Ann L. Fenwick
    • 4
  • Massimo Saccomani
    • 1
  1. 1.DAFNAE, Dipartimento di Agronomia Animali Alimenti Risorse Naturali e AmbienteUniversità degli Studi di PadovaLegnaroItaly
  2. 2.USDA-ARS, NPA, Sugarbeet Research UnitCrops Research LaboratoryFort CollinsUSA
  3. 3.USDA-ARSCrop Improvement and Protection Research UnitSalinasUSA
  4. 4.Beet Sugar Development FoundationCrops Research LaboratoryFort CollinsUSA

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