Plant Molecular Biology Reporter

, Volume 33, Issue 2, pp 316–325 | Cite as

Construction of a High-Density Simple Sequence Repeat Consensus Genetic Map for Pear (Pyrus spp.)

  • Hui Chen
  • Yue Song
  • Lei-Ting Li
  • M. Awais Khan
  • Xiu-Gen Li
  • Schuyler S. Korban
  • Jun Wu
  • Shao-Ling Zhang
Original Paper

Abstract

As an important fruit crop that is widely grown commercially in temperate regions of the world, pear (Pyrus) is a target for pursuing efficient breeding strategies. Construction of a reliable and dense genetic linkage map is critical for undertaking marker-assisted breeding. In this study, a population of 56 F1 seedlings of ‘Bayuehong’ × ‘Dangshansuli’ was used to construct a high-density simple sequence repeat (SSR)-based genetic linkage map. A total of 1,756 SSR markers, including 1,341 newly designed SSRs based on whole-genome sequencing of an Asiatic pear along with 415 previously reported SSRs, were first evaluated for polymorphism. Based on 894 SSRs demonstrating polymorphism, a consensus genetic map consisting of 734 loci distributed along all 17 linkage groups (LG) was constructed, with a total length of 1,661.4 cM and with an average marker interval of 2.26 cM. Comparisons among different maps of pear and apple were then made based on positions of previously mapped SSR markers on the consensus map. As a result, homologous linkage groups LG3 and LG11, LG5 and LG10, LG9 and LG17, LG13 and LG16, LG8 and LG15 have been identified. This high-density SSR map along with a set of SSR markers covering the whole genome of pear will greatly facilitate integration of independent maps, aid in pursuing comparative genome studies, and in evaluation of different germplasm in future genetic and breeding studies.

Keywords

Pear (Pyrus spp.) SSR Genetic map Comparative genetics 

Supplementary material

11105_2014_745_MOESM1_ESM.doc (76 kb)
Figure S1Alignment of our consensus map with other published pear maps using common SSRs. LG1 to17 are from the pear consensus map constructed in this study (Fig. 1), in the center; Ba1 to 17 are from the map of European pear ‘Bartlett’ (Ba) map (Yamamoto et al. 2007), on the left; Ho1 to 17 are from Asian pear ‘Hosui’ (Ho) map linkage groups (Terakami et al. 2009), on the right. The linkage groups show common markers among the three maps, and the interval distances between two adjacent markers. Markers in red are those located in different groups in our consensus map compared with those of other pear maps. (DOC 76 kb)
11105_2014_745_MOESM2_ESM.doc (107 kb)
Figure S2Comparison of the pear consensus map with ‘Fiesta’ (F) and ‘Co-op 17’ × ‘Co-op 16’ (Co) apple maps with common SSR markers. Apple ‘Fiesta’ (F) (Silfverberg-Dilworth et al. 2006) and ‘Co-op 17’ × ‘Co-op 16’ (Co) maps (Han et al. 2011) are shown on the left and right respectively, the pear consensus map (LG) (Fig. 1) is shown in the center. The linkage groups show common markers among the three maps and the interval distances between two adjacent markers. Markers in red are those that were located in different groups in our consensus map compared with those of other pear maps. (DOC 107 kb)
11105_2014_745_MOESM3_ESM.xls (440 kb)
ESM 3(XLS 440 kb)

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Hui Chen
    • 1
  • Yue Song
    • 1
  • Lei-Ting Li
    • 1
  • M. Awais Khan
    • 2
    • 4
  • Xiu-Gen Li
    • 3
  • Schuyler S. Korban
    • 2
    • 5
  • Jun Wu
    • 1
  • Shao-Ling Zhang
    • 1
  1. 1.College of Horticulture, State Key Laboratory of Crop Genetics and Germplasm EnhancementNanjing Agricultural UniversityNanjingChina
  2. 2.Department of Natural Resources and Environmental SciencesUniversity of IllinoisUrbanaUSA
  3. 3.Zhengzhou Fruit Research instituteCAASZhengzhouChina
  4. 4.International Potato Center (CIP)LimaPeru
  5. 5.Department of BiologyUniversity of Massachusetts-BostonBostonUSA

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