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Plant Molecular Biology Reporter

, Volume 33, Issue 1, pp 1–11 | Cite as

Characterization of DNA Methylation Variations During Fruit Development and Ripening of Sweet Orange

  • Jidi Xu
  • Haidan Xu
  • Qiang Xu
  • Xiuxin Deng
Original Paper

Abstract

DNA methylation is a type of epigenetic modification involved in many biological processes. Recent studies have shown that DNA methylation plays important roles in regulating fruit development and ripening. In the present study, sweet oranges at six developmental stages were investigated to understand the possible roles of DNA methylation during fruit development and ripening. Analysis of genomic DNA methylation levels in different fruit tissues and developmental stages indicated that methylation levels were not static during fruit development and ripening. To explore the possible contributions of methylation-related genes in fruit development and ripening process, the full-length coding sequences corresponding to three types of DNA methyltransferase families (CsMET1, CsCMT3, and CsDRM1), a chromatin-remodeling gene (CsDDM1), and a demethylation gene (CsDME1) were isolated. The expression profiles of these methylation-related genes were analyzed in vegetative tissues (seedlings and leaves) and reproductive tissues (three stages of flowering and six stages of fruit development). The results showed that CsDRM1 was highly expressed in seedlings, leaves, and flowers compared with other genes. During fruit development and ripening, CsMET1, CsCMT1, CsCMT2, CsDRM1, and CsDMEs showed higher expression in the peel than in the flesh. However, global DNA methylation level changes could not be explained by the expression of a single gene, suggesting that the methylation and demethylation system may cooperatively contribute to the final global DNA methylation pattern.

Keywords

Methyltransferase DNA methylation Citrus sinensis Fruit ripening 

Notes

Acknowledgments

This project was supported by the Ministry of Science and Technology of China (2011CB100600) and the National Natural Science Foundation of China (31272148 and 31222047).

Supplementary material

11105_2014_732_MOESM1_ESM.docx (13.4 mb)
ESM 1 (DOCX 13753 kb)

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  1. 1.Key Laboratory of Horticultural Plant Biology of Ministry of EducationHuazhong Agricultural UniversityWuhanPeople’s Republic of China

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