Plant Molecular Biology Reporter

, Volume 32, Issue 1, pp 270–281 | Cite as

Rice NARROW LEAF1 Regulates Leaf and Adventitious Root Development

  • Sung-Hwan Cho
  • Soo-Cheul Yoo
  • Haitao Zhang
  • Jung-Hyun Lim
  • Nam-Chon PaekEmail author
Original Paper


To improve our understanding of the molecular–genetic mechanisms governing leaf and root development in rice (Oryza sativa), we investigated narrow leaf1 (nal1), a pleiotropic mutant with short and narrow leaves, semi-dwarf stature, and fewer adventitious (or crown) roots. The narrow leaf5 (nal5) and nal1 mutants display similar defects in leaf and root development. Sequence analysis and complementation tests showed that nal5 is allelic to nal1. NAL1 encodes a putative trypsin-like serine/cysteine protease; the coding region of nal5 contains a missense mutation and nal1 harbors a 30-bp deletion. Quantitative real-time PCR revealed that nal1 mutants have altered expression levels of many OSHB, YABBY, and PIN-FORMED genes associated with leaf development and auxin transport. In addition, expression levels of CROWN ROOTLESS genes are markedly down-regulated in nal1. These results indicate that NAL1 functions in the regulation of both leaf and adventitious root development at the transcriptional level. Notably, exogenous auxin treatment rescued the reduced number of adventitious roots in nal1. Based on our results, we propose that NAL1 plays important roles in adventitious root development in rice.


Adventitious root Auxin transport CROWN ROOTLESS Narrow leaf1 Narrow leaf5 Rice 



We thank the Institute of Genetic Resource in the Kyushu University, Japan for kind donation of the rice nal1 and nal5 seeds. This work was supported by a grant from the Next-Generation BioGreen 21 Program (Plant Molecular Breeding Center No. PJ008128), Rural Development Administration, Republic of Korea.

Supplementary material

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Fig. S1 (DOCX 484 kb)
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Fig. S2 (DOCX 54 kb)
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Table S1 (DOCX 23 kb)
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Table S2 (DOCX 23 kb)
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Table S3 (DOCX 23 kb)


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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Sung-Hwan Cho
    • 1
  • Soo-Cheul Yoo
    • 1
  • Haitao Zhang
    • 1
  • Jung-Hyun Lim
    • 1
  • Nam-Chon Paek
    • 1
    • 2
    Email author
  1. 1.Department of Plant Science, Plant Genomics and Breeding Institute, and Research Institute for Agriculture and Life SciencesSeoul National UniversitySeoulRepublic of Korea
  2. 2.Institute of Green Bio Science and TechnologySeoul National UniversityPyeongchangRepublic of Korea

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