Plant Molecular Biology Reporter

, Volume 31, Issue 3, pp 678–687 | Cite as

An AFLP, SRAP, and SSR Genetic Linkage Map and Identification of QTLs for Fruit Traits in Pear (Pyrus L.)

Original Paper

Abstract

In this study, a population of 97 F1 seedlings from a cross between the interspecific hybrid (European × Chinese species) pear ‘Bayuehong’ (BYH) and the Chinese pear ‘Dangshansuli’ (DS) was used for establishing linkage maps and for quantitative trait loci (QTL) discovery. Using amplified length polymorphism (AFLP), simple sequence repeat (SSR), and sequence-related amplified polymorphism (SRAP) markers, along with the S locus for self-incompatibility, two parental linkage maps were constructed. The map of BYH consisted of 214 markers (143 AFLPs, 64 SRAPs, 6 SSRs, and S) mapped on all 17 linkage groups of the pear genome with a total length of 1,352.7 cM. The map of DS was comprised of 122 markers (83 AFLPs, 37 SRAPs, 1 SSR, and S) distributed along all 17 linkage groups and covering 1,044.3 cM. Based on phenotypic data from two successive years (2007 and 2008) for six fruit traits, including fruit weight (in grams), fruit diameter (in centimeters), fruit length (in centimeters), soluble solids content, fruit shape index, and maturity date, 19 QTLs were detected. These QTLs were mapped on LG 01, LG 02, LG 05, LG 07, LG 08, LG 10 of the BYH map and LG 02, LG 06, LG 15 of the DS map and accounting for 7.1 to 22.0 % of the observed phenotypic variance. Four QTLs, Pfi-8-1 for fruit shape index, Pfm-8-1 for fruit maturity date, Pfw-7-1 and Pfw-8-1 for fruit weight (in grams), with LOD scores ≥3.5, were deemed as major genes. QTLs Pfi-8-1, Pfm-8-1, and Pfw-8-1 were co-localized on LG 08 of the BYH map, along with Pfl-8-1 for fruit length. It was observed that on LG 07 of the BYH map, QTLs for fruit length, fruit shape index, and fruit weight were clustered. When QTL locations from both years were compared, Pfl-7-1 and Pfl-7-2 for fruit length, Pfi-2-1 and Pfi-2-2 for fruit shape index, and Pfm-8-1 and Pfm-8-2 for fruit maturity date were stably mapped onto the same linkage groups, respectively. Moreover, Pfm-8-1 and Pfm-8-2 were also located within the same region of LG 08 of the BYH map.

Keywords

Pear Molecular markers Linkage map Fruit traits QTL 

Supplementary material

11105_2012_544_MOESM1_ESM.doc (32 kb)
Supplementary Table 1Data on fruit traits collected from an F1 population of ‘Bayuehong’ × ‘Dangshansuli’ in two successive years (2007 and 2008). (DOC 32 kb)
11105_2012_544_MOESM2_ESM.doc (55 kb)
Supplementary Table 2Pearson correlation between phenotypic fruit quality traits of ‘Bayuehong’ × ‘Dangshansuli’ F1 population collected over 2 years (2007 and 2008). (DOC 55 kb)

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Copyright information

© Springer Science+Business Media New York 2012

Authors and Affiliations

  • Rui-ping Zhang
    • 1
  • Jun Wu
    • 1
  • Xiu-gen Li
    • 2
  • M. Awais Khan
    • 3
  • Hui Chen
    • 1
  • Schuyler S. Korban
    • 3
  • Shao-ling Zhang
    • 1
  1. 1.College of HorticultureNanjing Agricultural UniversityNanjingChina
  2. 2.The National Institute of Fruit Tree ScienceZhengzhouChina
  3. 3.Department of Natural Resources and Environmental SciencesUniversity of IllinoisUrbanaUSA

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