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Plant Molecular Biology Reporter

, Volume 31, Issue 1, pp 141–150 | Cite as

Changes in the Activity and Transcription of Antioxidant Enzymes in Response to Al Stress in Black Soybeans

  • Konghuan Wu
  • Suqin Xiao
  • Qi Chen
  • Qifeng Wang
  • Yanan Zhang
  • Kunzhi Li
  • Yongxiong Yu
  • Limei ChenEmail author
Original Paper

Abstract

In this study, the effects of Al stress on the activity and transcription of antioxidant enzymes were investigated in an acid-resistant black soybean (RB) and an acid-sensitive black soybean (SB) under hydroponic conditions to further clarify the role of antioxidant enzymes in the plant’s response to Al stress. The results indicated that oxidative stress was induced in the roots and leaves of RB and SB and that the stress level was higher in SB than in RB. Changes in the catalase (CAT) activity in response to Al stress occurred faster in RB roots and leaves than in SB. As the duration of Al stress increased, the peroxidase (POD) activity was enhanced more pronouncedly in RB roots and leaves than in SB. The activity of superoxide dismutase (SOD) in the roots and leaves of RB and SB was not responsive to Al stress. A high transcription level of a selected POD gene was detected in RB leaves, but no transcription of this POD gene was observed in SB leaves under Al stress. Moreover, the transcription level of this POD gene was higher in RB roots than in SB roots. Under Al stress, the transcription of two selected SOD genes showed an increasing trend in RB but decreased in SB. Furthermore, the transcription levels of these two selected SOD genes were always higher in RB than in SB. The above results suggest that not only does RB have a higher level of antioxidant enzyme activities but also that antioxidant enzyme genes can be upregulated by Al stress. This may be an important mechanism for RB to deal with oxidative stress induced by Al toxicity.

Keywords

Black soybean Al toxicity Oxidative stress Antioxidant enzymes Gene transcription 

Notes

Acknowledgments

This work was supported in part by grants from the National Basic Research Program of China (No. 2007CB108901) and the Foundation (2004PY01-5) of Yunnan Province and Kunming University of Science and Technology for Training Adult and Young Leaders of Science and Technology.

References

  1. Aebi H (1984) Catalase in vitro. Methods Enzymol 105:121–126PubMedCrossRefGoogle Scholar
  2. Aftab T, Khan MMA, Idress M, Naeem M, Moinuddin (2010) Effectes of aluminum exposures on growth, phostosynthetic efficiency, lipid peroxidation, antioxidation, antioxidant enzymes and artemisinin content of Artemisia annua L. J Phytol 2:23–37Google Scholar
  3. Alscher RG, Donahue JL, Cramer CL (1997) Reactive oxygen species and antioxidants: relationships in green cells. Physiol Plant 100:224–233CrossRefGoogle Scholar
  4. Barcelo J, Poschenrieder C (2002) Fast root growth responses, root exudates, and internal detoxification as clues to the mechanisms of aluminium toxicity and resistance: a review. Environ Exp Bot 48:75–92CrossRefGoogle Scholar
  5. Basu U, Good A, Taylor G (2001) Transgenic Brassica napus plants overexpressing aluminium-induced mitochondrial manganese superoxide dismutase cDNA are resistant to aluminium. Plant Cell Environ 24:1269–1278CrossRefGoogle Scholar
  6. Boscolo PRS, Menossi M, Jorge RA (2003) Aluminum-induced oxidative stress in maize. Phytochemistry 62:181–189PubMedCrossRefGoogle Scholar
  7. Bowler C, Montagu M, Inze D (1992) Superoxide dismutase and stress tolerance. Annu Rev Plant Biol 43:83–116CrossRefGoogle Scholar
  8. Cakmak I, Horst WJ (1991) Effect of aluminium on lipid peroxidation, superoxide dismutase, catalase, and peroxidase activities in root tips of soybean (Glycine max). Physiol Plant 83:463–468CrossRefGoogle Scholar
  9. Chance B, Maehly A (1955) Assay of catalases and peroxidases. Methods Enzymol 2:764–775CrossRefGoogle Scholar
  10. Chen WR, Liu P, Xu GD, Yu HN, Dong L (2008) Effects of applicated on alleviation of aluminum toxicity to soybean (in Chinese). J Zhenjiang Normal Univ 131:201–207Google Scholar
  11. Cohu CM, Pilon M (2007) Regulation of superoxide dismutase expression by copper availability. Physiol Plant 129:747–755CrossRefGoogle Scholar
  12. de Azevedo Neto AD, Prisco JT, Enéas-Filho J, Rolim Medeiros JV, Gomes-Filho E (2005) Hydrogen peroxide pre-treatment induces salt-stress acclimation in maize plants. J Plant Physiol 162:1114–1122PubMedCrossRefGoogle Scholar
  13. Dechassa D, Khairy S, Zachary S (2011) Proteomic analysis of soybean roots under aluminum stress. Int J Plant Genom 2011:1–12Google Scholar
  14. Diao G, Wang Y, Wang C, Yang C (2011) Cloning and functional characterization of a novel glutathione S-transferase gene from Limonium bicolor. Plant Mol Biol Rep 29:77–87CrossRefGoogle Scholar
  15. Du B, Nian H, Zhang Z, Yang C (2010) Effects of aluminum on superoxide dismutase and peroxidase activities, and lipid peroxidation in the roots and calluses of soybeans differing in aluminum tolerance. Acta Physiologiae Plantarum 32:883–890CrossRefGoogle Scholar
  16. Elstner EF (1982) Oxygen activation and oxygen toxicity. Annu Rev Plant Physiol 33:73–96CrossRefGoogle Scholar
  17. Ezaki B, Gardner RC, Ezaki Y, Matsumoto H (2000) Expression of aluminum-induced genes in transgenic Arabidopsis plants can ameliorate aluminum stress and/or oxidative stress. Plant Physiol 122:657–666PubMedCrossRefGoogle Scholar
  18. Gay CA, Gebicki JM (2003) Measurement of protein and lipid hydroperoxides in biological systems by the ferric-xylenol orange method. Anal Biochem 315:29–35PubMedCrossRefGoogle Scholar
  19. Giannopolitis CN, Ries SK (1977) Superoxide dismutases: I. Occurrence in higher plants. Plant Physiol 59:309PubMedCrossRefGoogle Scholar
  20. Ikegawa H, Yamamoto Y, Matsumoto H (2000) Responses to aluminium of suspension-cultured tobacco cells in a simple calcium solution. Soil Sci Plant Nutr 46:503–514Google Scholar
  21. Kurepa J, Van Montagu M, Inzé D (1997) Expression of sodCp and sodB genes in Nicotiana tabacum: effects of light and copper excess. J Exp Bot 48:2007–2014Google Scholar
  22. Kim YJ, Shim JS, Krishna PR, Kim SY, In JG, Kim MK, Yang DC (2008) Isolation and characterization of a glutaredoxin gene from Panax ginseng CA Meyer. Plant Mol Biol Rep 26:335–349CrossRefGoogle Scholar
  23. Liao H, Wan H, Shaff J, Wang X, Yan X, Kochian LV (2006) Phosphorus and aluminum interactions in soybean in relation to aluminum tolerance. Exudation of specific organic acids from different regions of the intact root system. Plant Physiol 141:674–684PubMedCrossRefGoogle Scholar
  24. Madamanchi NR, Donahue JL, Cramer CL, Alscher RG, Pedersen K (1994) Differential response of Cu, Zn superoxide dismutases in two pea cultivars during a short-term exposure to sulfur dioxide. Plant Mol Biol 26:95–103PubMedCrossRefGoogle Scholar
  25. Meriga B, Krishna Reddy B, Rajender Rao K, Ananda Reddy L, Kavi Kishor P (2004) Aluminium-induced production of oxygen radicals, lipid peroxidation and DNA damage in seedlings of rice (Oryza sativa). J Plant Physiol 161:63–68PubMedCrossRefGoogle Scholar
  26. Molassiotis A, Sotiropoulos T, Tanou G, Diamantidis G, Therios I (2006) Boron-induced oxidative damage and antioxidant and nucleolytic responses in shoot tips culture of the apple rootstock EM 9 (Malus domestica Borkh). Environ Exp Bot 56:54–62CrossRefGoogle Scholar
  27. Pawlak S, Firych A, Rymer K, Deckert J (2009) Cu, Zn-superoxide dismutase is differently regulated by cadmium and lead in roots of soybean seedlings. Acta Physiologiae Plantarum 31:741–747CrossRefGoogle Scholar
  28. Sakaguchi S, Fukuda T, Takano H, Ono K, Takio S (2004) Photosynthetic electron transport differentially regulates the expression of superoxide dismutase genes in liverwort, Marchantia paleacea var. diptera. Plant Cell Physiol 45:318PubMedCrossRefGoogle Scholar
  29. Sunkar R, Kapoor A, Zhu JK (2006) Posttranscriptional induction of two Cu/Zn superoxide dismutase genes in Arabidopsis is mediated by downregulation of miR398 and important for oxidative stress tolerance. The Plant Cell Online 18:2051–2065CrossRefGoogle Scholar
  30. Song H, Fan P, Li Y (2009) Overexpression of organellar and cytosolic AtHSP90 in Arabidopsis thaliana impairs plant tolerance to oxidative stress. Plant Mol Biol Rep 27:342–349CrossRefGoogle Scholar
  31. Taylor GJ, Basu A, Basu U, Slaski JJ, Zhang G, Good A (1997) Al-induced, 51-kilodalton, membrane-bound proteins are associated with resistance to Al in a segregating population of wheat. Plant Physiol 114:363–372PubMedGoogle Scholar
  32. Tsugane K, Kobayashi K, Niwa Y, Ohba Y, Wada K, Kobayashi H (1999) A recessive Arabidopsis mutant that grows photoautotrophically under salt stress shows enhanced active oxygen detoxification. The Plant Cell Online 11:1195–1206Google Scholar
  33. Wang YS, Wang J, Yang ZM, Wang QY, Lu B, Li SQ, Lu YP, Wang SH, Sun X (2004) Salicylic acid modulates aluminum-induced oxidative stress in roots of Cassia tora. Acta Bot Sin-Eng Edn 46:819–828Google Scholar
  34. Willekens H, Chamnongpol S, Davey M, Schraudner M, Langebartels C, Van Montagu M, Inzé D, Van Camp W (1997) Catalase is a sink for H2O2 and is indispensable for stress defence in C3 plants. EMBO J 16:4806–4816PubMedCrossRefGoogle Scholar
  35. Williams RJ (1999) What is wrong with aluminum? The J.D. Birchall memorial lecture. J Inorg Biochem 76:81–88PubMedCrossRefGoogle Scholar
  36. Yamamoto Y, Kobayashi Y, Devi SR, Rikiishi S, Matsumoto H (2002) Aluminum toxicity is associated with mitochondrial dysfunction and the production of reactive oxygen species in plant cells. Plant Physiol 128:63–72PubMedCrossRefGoogle Scholar
  37. Yamamoto Y, Kobayashi Y, Matsumoto H (2001) Lipid peroxidation is an early symptom triggered by aluminum, but not the primary cause of elongation inhibition in pea roots. Plant Physiol 125:199–208PubMedCrossRefGoogle Scholar
  38. Yamasaki H, Abdel-Ghany SE, Cohu CM, Kobayashi Y, Shikanai T, Pilon M (2007) Regulation of copper homeostasis by micro-RNA in Arabidopsis. J Biol Chem 282:16369–16378PubMedCrossRefGoogle Scholar
  39. Zhang ZL, Qu WJ (2003) The experimental guide for plant physiology (in Chinese). Higher Education Press, BeijingGoogle Scholar

Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Konghuan Wu
    • 1
  • Suqin Xiao
    • 1
  • Qi Chen
    • 1
  • Qifeng Wang
    • 2
  • Yanan Zhang
    • 1
  • Kunzhi Li
    • 1
  • Yongxiong Yu
    • 2
  • Limei Chen
    • 1
    Email author
  1. 1.Faculty of Life Science and Biotechnology, Chenggong CampusKunming University of Science and TechnologyChenggongChina
  2. 2.College of Zoological Science and TechnologySouthwest UniversityChongqingChina

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