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Plant Molecular Biology Reporter

, Volume 31, Issue 1, pp 98–108 | Cite as

BEAK LIKE SPIKELET1 is Required for Lateral Development of Lemma and Palea in Rice

  • Xiaoding Ma
  • Zhijun Cheng
  • Fuqing Wu
  • Mingna Jin
  • Liguo Zhang
  • Feng Zhou
  • Jiulin Wang
  • Kunneng Zhou
  • Jian Ma
  • Qibing Lin
  • Cailin Lei
  • Jianmin WanEmail author
Original Paper

Abstract

Lemma and palea are unique floral structures found only in Poaceae, and are responsible for protecting the inner floral organs and kernels from environmental stresses. However, the mechanism underlying specification of their morphology remains unclear. In this study, we characterized a rice mutant, beak like spikelet1 (bls1), which specifically affects development of the lemma and palea. In bls1 mutant, floral-organ identity and floral-organ patterning are normal, and the defects occur at the stage of the lemma and palea expansion, whereas the other aspects of floral architecture and form are not affected. We isolated BLS1 by positional cloning and found that it encodes a protein with a conserved domain of unknown function. BLS1 is expressed strongly in young inflorescence, specifically the young lemmas and paleas of spikelets. Subcellular localization analysis showed that BLS1 is localized in the nucleus. Expression of the AP1-like and SEP-like floral homeotic genes were not changed in the bls1 mutant. Our study suggested that BLS1 is required for lateral development of the lemma and palea and does not function at stages of floral-organ initiation and patterning.

Keywords

ALOG Floral-organ Cell expansion Oryza sativa

Abbreviations

SEM

Scanning electron microscope

AP

APETALA

SEP

SEPALLATA

PI

PISTILLATA

AG

AGAMOUS

MADS

MCM1-AGAMOUS-DEFICIENS-SRF

qRT-PCR

Quantitative reverse transcription polymerase chain reaction

ALOG

Arabidopsis LSH1 and Oryza G1

Notes

Acknowledgments

We thank for Drs. Xianchun Xia (Institute of Crop Science, CAAS) and Zhigang Zhao (Nanjing Agricultural University) for their critical reading of the manuscript. This research was supported by grants from the Chinese ‘973’ Program (2010CB125904-4) and National Transform Science and Technology Program (2009ZX0809-104B)

Supplementary material

11105_2012_480_MOESM1_ESM.doc (123 kb)
Supplemental Fig. 1. Comparison of spikelet hull width and length in WT and bls1-1 just before heading (n =12 plants, 20 spikelets/plant). The spikelet hull width is reduced significantly compared with WT, while the spikelet hull length showed no difference. All data are given as mean ± SD, *P < 0.01 (DOC 123 kb)
11105_2012_480_MOESM2_ESM.doc (174 kb)
Supplemental Fig. 2. Identification of deletion site in bls1-2. Identification of deletion site in bls1-2 by PCR amplification with four primer pairs, M6, q8F-q8R, q14F-q14R, and M6F-q14R indicated in Fig. 4a. Note that primer pair q8F-8R can amplify from WT (a), and primer combination of M6F-q14R from bls1-2 shown by the arrow in (b) (DOC 174 kb)
11105_2012_480_MOESM3_ESM.doc (636 kb)
Supplemental Fig. 3. CDS and protein sequences of BLS1. Green lines and red box indicate the ALOG domain and nuclear localization signal, respectively (DOC 636 kb)
11105_2012_480_MOESM4_ESM.doc (64 kb)
Supplemental Table 1. Sequences of the primers used in this study(DOC 63 kb)

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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Xiaoding Ma
    • 1
  • Zhijun Cheng
    • 1
  • Fuqing Wu
    • 1
  • Mingna Jin
    • 1
  • Liguo Zhang
    • 2
  • Feng Zhou
    • 2
  • Jiulin Wang
    • 1
  • Kunneng Zhou
    • 2
  • Jian Ma
    • 1
  • Qibing Lin
    • 1
  • Cailin Lei
    • 1
  • Jianmin Wan
    • 1
    • 2
    Email author
  1. 1.National Key Facility for Crop Gene Resources and Genetic ImprovementInstitute of Crop Science, Chinese Academy of Agricultural SciencesBeijingChina
  2. 2.National Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research CenterNanjing Agricultural UniversityNanjingChina

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