Plant Molecular Biology Reporter

, Volume 30, Issue 6, pp 1322–1328 | Cite as

Universal ITS2 Barcoding DNA Region Coupled with High-Resolution Melting (HRM) Analysis for Seed Authentication and Adulteration Testing in Leguminous Forage and Pasture Species

  • Ioannis Ganopoulos
  • Panagiotis Madesis
  • Athanasios Tsaftaris
Original Paper

Abstract

Legume species are part of a very important agricultural family, second only to cereals. Their importance for sustainable agriculture worldwide comes from their nitrogen-fixing ability. They include mainly annual grain crops and also very important perennial forage and pasture species. Given their small size, seed admixture and adulteration are a common problem, lowering the forage value, creating weed components in the grassland and causing digestive problems to animals. Here we report the application of the Barcode-DNA High-Resolution Melting (Bar-HRM) analysis method using the universal nuclear plant DNA barcoding region ITS2 for the identification, adulteration and quantification of the main pasture species. Bar-HRM detected Medicago lupulina adulterants in Trifolium pratense seeds as low as 1:100. In conclusion, Bar-HRM analysis could be a faster with higher resolution and cost-effective alternative method to authenticate forage and pasture species and quantitatively detect the purity of their seeds or their feed products.

Keywords

Forage Pasture Seed authentication Adulteration Bar-HRM 

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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Ioannis Ganopoulos
    • 1
    • 2
  • Panagiotis Madesis
    • 2
  • Athanasios Tsaftaris
    • 1
    • 2
  1. 1.Department of Genetics and Plant Breeding, School of AgricultureAristotle University of ThessalonikiThessalonikiGreece
  2. 2.Institute of Agrobiotechnology, CERTHThermiGreece

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