Plant Molecular Biology Reporter

, Volume 30, Issue 5, pp 1210–1217 | Cite as

Cloning and Characterization of the Wx Gene Encoding a Granule-Bound Starch Synthase in Lotus (Nelumbo nucifera Gaertn)

  • Ye Lu
  • Liangjun LiEmail author
  • Yong Zhou
  • Qingsong Gao
  • Guohua Liang
  • Xuehao Chen
  • Xiaohua Qi
Original Paper


The granule-bound starch synthase (GBSS) proteins were widely considered as one of the most important enzymes in plant amylose synthesis. However, understanding of the molecular basis of the GBSS protein in lotus remains fragmented. In this work, a lotus Wx gene, encoding a GBSS (GenBank accession no. EU938541), was isolated and characterized. This gene comprises 13 exons and 12 introns and covers 4152 bp (GenBank accession no. FJ602702). The exons of Wx gene have similar lengths, while the introns vary greatly. Phylogenetic tree indicated that the lotus GBSS protein belongs to a GBSS I subgroup. The expression of the Wx gene varies in different organs of the lotus during its development process and is also expressed differently in different cultivars. The Wx gene is expressed at a higher level in the rhizomes of cultivar Meirenhong than in those of cultivar Elian 4. This study elucidates more molecular information about the Wx gene in lotus and provides a theoretical foundation for the genes regulation and the modification of starch quality.


Lotus (Nelumbo nucifera Gaertn) Wx gene Granule-bound starch synthase Cloning Expression analysis 



This work was supported by National Natural Science Foundation of China (31071795) and Special Fund for Agro-scientific Research in the Public Interest of China (200903017-02).


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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Ye Lu
    • 1
    • 2
  • Liangjun Li
    • 1
    • 2
    Email author
  • Yong Zhou
    • 1
  • Qingsong Gao
    • 1
  • Guohua Liang
    • 1
  • Xuehao Chen
    • 2
  • Xiaohua Qi
    • 2
  1. 1.Key Laboratory of the Ministry of Education for Plant Functional GenomicsYangzhou UniversityYangzhouChina
  2. 2.Laboratory of Aquatic VegetablesYangzhou UniversityYangzhouChina

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