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Plant Molecular Biology Reporter

, Volume 30, Issue 4, pp 860–866 | Cite as

Molecular Cloning and Characterization of BcMF22, a Novel Gene Related to Pollen Development and Fertilization in Brassica campestris ssp. chinensis

  • Yifei Li
  • Lin Qiu
  • Li Huang
  • Jiashu CaoEmail author
Article

Abstract

The BcMF22 (Brassica campestris Male Fertility 22) gene was isolated from Chinese cabbage-pak-choi (B. campestris L. ssp. chinensis Makino, syn. B. rapa ssp. chinensis). The cDNA clone of BcMF22 was 1,870 bp in length and contained an open reading frame (OFF) of 1,260 bp, while the genomic sequence contained no introns. Sequence prediction indicated that BcMF22 might encode a methytransferase. Spatial and temporal expression analyses indicated BcMF22 was preferentially expressed in pollen. Transcripts of BcMF22 were first detected in pollen mother cells and continued to be present in mature pollen. In addition, transcripts were detected in pollinated pistils, approximately 4 h after pollination (4HAP). These results indicated that BcMF22 might be a pollen-preferential gene and was closely involved in pollination.

Keywords

Brassica campestris ssp. chinensis BcMF22 Pollen Pistil HAP 

Abbreviations

BcMF22

Brassica campestris Male Fertility 22

ORF

Open reading frame

RACE

Rapid amplification of cDNA ends

HAP

Time after pollination

qRT-PCR

Quantitative real-time RT-PCR

Notes

Acknowledgement

This work was supported by the Natural Science Foundation of China (No. 30871715), the Natural Science Foundation of Zhejiang Province (2010 C12004) and the Key Sci-Technology Project of Zhejiang Province (No. Y3100300).

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  1. 1.Laboratory of Cell and Molecular Biology, Institute of Vegetable ScienceZhejiang UniversityHangzhouPeople’s Republic of China

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