Plant Molecular Biology Reporter

, Volume 30, Issue 3, pp 529–538 | Cite as

BcJMJ30, the Gene Encoding jmjC Domain-Containing Histone Demethylase Is Associated with Pollen Development and Fertilization in Brassica campestris ssp. chinensis

  • Yifei Li
  • Lin Qiu
  • Li Huang
  • Jiashu CaoEmail author


Histone methylation plays an important role in regulating gene expression, and it can act in epigenetic memory system, thus deciding fates of cells and their identities. The jumonji C (jmjC) domain-containing proteins have been shown to reverse histone methylation in nonplant model systems. However, knowledge of the jmjC domain-containing histone demethylase that is involved in pollen development and fertilization in plants remains limited. In this study, BcJMJ30 (Brassica campestris Jumonji 30), encoding a putative jmjC domain-containing protein that is up-regulated after pollination of sterile pistils and fertile flower buds, has been cloned in the genic male sterile AB line (Bajh97-01A/B) of Chinese cabbage-pak-choi (B. campestris L. ssp. chinensis Makino). Phylogenetic analysis has revealed that the BcJMJ30 was closely related to At3g20810 (AtJMJ30) in Arabidopsis, which belongs to the JMJD5 subgroup of the jmjC domain-only group. Spatial and temporal expression patterns analysis by RT-PCR and qRT-PCR indicates that transcripts of BcJMJ30 was preferentially expressed in stamens of fertile flower buds and are steadily expressed in pistils following pollination. In situ hybridization results have revealed that BcJMJ30 is expressed in both pollen and tapetum. In general, these results indicated that BcJMJ30 is a novel gene, with a jmjC domain-only that may be involved in pollen development and fertilization.


Brassica campestris ssp. chinensis Pollen Pistil BcJMJ30 Histone demethylase jmjC 



Brassica campestris Jumonji 30


Open reading frame


Rapid amplification of cDNA ends


Time after pollination


Semi-quantitative reverse transcriptase PCR



This work was supported by the Key Sci-Technology Project of Zhejiang Province (No. 2010 C12004) and the Natural Science Foundation of Zhejiang Province (Y3100300).


  1. Alam MM, Sharmin S, Nabi Z, Mondal SI, Islam MS, Nayeem SB, Shoyaib M, Khan H (2010) A putative leucine-rich repeat receptor-like kinase of jute involved in stress response. Plant Mol Biol Rep 28:394–402CrossRefGoogle Scholar
  2. Bian F, Zheng C, Qu F, Gong X, You C (2010) Proteomic analysis of somatic embryogenesis in Cyclamen persicum Mill. Plant Mol Biol Rep 28:364–364CrossRefGoogle Scholar
  3. Boavida LC, Borges F, Becker JD, Feijo JA (2011) Whole genome analysis of gene expression reveals coordinated activation of signaling and metabolic pathways during pollen-pistil interactions in Arabidopsis. Plant Physiol 155:2066–2080PubMedCrossRefGoogle Scholar
  4. Chapman LA, Goring DR (2010) Pollen–pistil interactions regulating successful fertilization in the Brassicaceae. J Exp Bot 61:1987–1999PubMedCrossRefGoogle Scholar
  5. de Oliveira RR, Chalfun-Junior A, Paiva LV, Andrade AC (2010) In silico and quantitative analyses of MADS-Box genes in Coffea arabica. Plant Mol Biol Rep 28:460–472CrossRefGoogle Scholar
  6. Demetriou K, Kapazoglou A, Bladenopoulos K, Tsaftaris AS (2010) Epigenetic chromatin modifiers in barley: II. Characterization and expression analysis of the HDA1 family of barley histone deacetylases during development and in response to jasmonic acid. Plant Mol Biol Rep 28:9–21CrossRefGoogle Scholar
  7. George S, Parida A (2010) Characterization of an oxidative stress inducible nonspecific lipid transfer protein coding cDNA and its promoter from drought tolerant plant Prosopis juliflora. Plant Mol Biol Rep 28:32–40CrossRefGoogle Scholar
  8. Gulyas G, Shin Y, Kim H, Lee JS, Hirata Y (2010) Altered transcript reveals an Orf507 sterility-related gene in Chili Pepper (Capsicum annuum L.). Plant Mol Biol Rep 28:605–612CrossRefGoogle Scholar
  9. He J, Kallin EM, Tsukada Y, Zhang Y (2008) The H3K36 demethylase Jhdm1b/Kdm2b regulates cell proliferation and senescence through p15(Ink4b). Nat Struct Mol Biol 15:1169–1175PubMedCrossRefGoogle Scholar
  10. Hong EH, Jeong YM, Ryu JY, Amasino RM, Noh B, Noh YS (2009) Temporal and spatial expression patterns of nine Arabidopsis genes encoding Jumonji C-domain proteins. Mol Cells 27:481–490PubMedCrossRefGoogle Scholar
  11. Hsia DA, Tepper CG, Pochampalli MR, Hsia EY, Izumiya C, Huerta SB, Wright ME, Chen HW, Kung HJ, Izumiya Y (2010) KDM8, a H3K36me2 histone demethylase that acts in the cyclin A1 coding region to regulate cancer cell proliferation. PNAS 107:9671–9676PubMedCrossRefGoogle Scholar
  12. Huang L, Cao J, Ye W, Liu T, Jiang L, Ye Y (2008) Transcriptional differences between the male-sterile mutant bcms and wild-type Brassica campestris ssp. chinensis reveal genes related to pollen development. Plant Biol (Stuttg) 10:342–355CrossRefGoogle Scholar
  13. Huang L, Liu Y, Yu X, Xiang X, Cao J (2011) A polygalacturonase inhibitory protein gene (BcMF19) expressed during pollen development in Chinese cabbage-pak-choi. Mol Biol Rep 38:545–552PubMedCrossRefGoogle Scholar
  14. Huang L, Zhao X, Lu T, Dong H, Cao, J (2010) Developmental characteristics of floral organs and pollen of Chinese cabbage (Brassica campestris L. ssp. chinensis). Plant Syst Evol 286:103–115CrossRefGoogle Scholar
  15. Johnson L, Mollah S, Garcia BA, Muratore TL, Shabanowitz J, Hunt DF, Jacobsen SE (2004) Mass spectrometry analysis of Arabidopsis histone H3 reveals distinct combinations of post-translational modifications. Nucleic Acids Res 32:6511–6518PubMedCrossRefGoogle Scholar
  16. Kato H, Xie G, Sato Y, Imai R (2010) Isolation of anther-specific gene promoters suitable for transgene expression in rice. Plant Mol Biol Rep 28:381–387CrossRefGoogle Scholar
  17. Klose RJ, Kallin EM, Zhang Y (2006) JmjC-domain-containing proteins and histone demethylation. Nat Rev Genet 7:715–727PubMedCrossRefGoogle Scholar
  18. Kouzarides T (2007) Chromatin modifications and their function. Cell 128:693–705PubMedCrossRefGoogle Scholar
  19. Lee MG, Wynder C, Cooch N, Shiekhattar R (2005) An essential role for CoREST in nucleosomal histone 3 lysine 4 demethylation. Nature 437:432–435PubMedGoogle Scholar
  20. Liu YG, Whittier RF (1995) Thermal asymmetric interlaced PCR: automatable amplification and sequencing of insert end fragments from P1 and YAC clones for chromosome walking. Genomics 25:674–681.PubMedCrossRefGoogle Scholar
  21. Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2-Δ-ΔCT method. Methods 25: 402–408.PubMedCrossRefGoogle Scholar
  22. Lu F, Li G, Cui X, Liu C, Wang XJ, Cao X (2008) Comparative analysis of JmjC domain-containing proteins reveals the potential histone demethylases in Arabidopsis and rice. J Integr Plant Biol 50:886–896PubMedCrossRefGoogle Scholar
  23. Lu F, Cui X, Zhang S, Liu C, Cao X (2010) JMJ14 is an H3K4 demethylase regulating flowering time in Arabidopsis. Cell Res 20:387–390PubMedCrossRefGoogle Scholar
  24. Metzger E, Wissmann M, Yin N, Muller JM, Schneider R, Peters AH, Gunther T, Buettner R, Schule R (2005) LSD1 demethylates repressive histone marks to promote androgen-receptor-dependent transcription. Nature 437:436–439PubMedGoogle Scholar
  25. Nawaschin S (1898) Resultate einer revision der befruchtungsvorgänge bei lilium martagon und fertillaria tenella. Bulletin de l’Académie Impériale des Sciences 9:377–382Google Scholar
  26. Noh B, Lee SH, Kim HJ, Yi G, Shin EA, Lee M, Jung KJ, Doyle MR, Amasino RM, Noh YS (2004) Divergent roles of a pair of homologous jumonji/zinc-finger-class transcription factor proteins in the regulation of Arabidopsis flowering time. Plant Cell 16:2601–2613PubMedCrossRefGoogle Scholar
  27. Pagnussat GC, Yu HJ, Ngo QA, Rajani S, Mayalagu S, Johnson CS, Capron A, Xie L, Ye D, Sundaresan V (2005) Genetic and molecular identification of genes required for female gametophyte development and function in Arabidopsis. Development 132:603–614PubMedCrossRefGoogle Scholar
  28. Porebski S, Bailey LG, Baum BR (1997) Modification of a CTAB DNA extraction protocol for plants containing high polysaccharide and polyphenol components. Plant Mol Biol Rep 15:8–15CrossRefGoogle Scholar
  29. Su V, Hsu BD (2010) Transient expression of the cytochrome p450 CYP78A2 enhances anthocyanin production in flowers. Plant Mol Biol Rep 28:302–308CrossRefGoogle Scholar
  30. Sun Q, Zhou DX (2008) Rice jmjC domain-containing gene JMJ706 encodes H3K9 demethylase required for floral organ development. PNAS 105:13679–13684PubMedCrossRefGoogle Scholar
  31. Tsukada Y, Fang J, Erdjument-Bromage H, Warren ME, Borchers CH, Tempst P, Zhang Y (2006) Histone demethylation by a family of JmjC domain-containing proteins. Nature 439:811–816PubMedCrossRefGoogle Scholar
  32. Whetstine JR, Nottke A, Lan F, Huarte M, Smolikov S, Chen Z, Spooner E, Li E, Zhang G, Colaiacovo M, Shi Y (2006) Reversal of histone lysine trimethylation by the JMJD2 family of histone demethylases. Cell 125:467–481PubMedCrossRefGoogle Scholar
  33. Zhang K, Sridhar VV, Zhu J, Kapoor A, Zhu JK (2007) Distinctive core histone post-translational modification patterns in Arabidopsis thaliana. PLoS One 2:e1210PubMedCrossRefGoogle Scholar
  34. Zhao Z, Yu Y, Meyer D, Wu C, Shen WH (2005) Prevention of early flowering by expression of FLOWERING LOCUS C requires methylation of histone H3 K36. Nat Cell Biol 7:1256–1260PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  1. 1.Laboratory of Cell and Molecular Biology, Institute of Vegetable ScienceZhejiang UniversityHangzhouPeople’s Republic of China

Personalised recommendations