Determination S-Genotypes and Identification of Five Novel S-RNase Alleles in Wild Malus Species
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- Li, T., Long, S., Li, M. et al. Plant Mol Biol Rep (2012) 30: 453. doi:10.1007/s11105-011-0345-y
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Apple (Malus × domestica Borkh.) is a typical Rosaceae species that exhibits gametophytic self-incompatibility (GSI) controlled by polymorphic S-alleles. In this study, the S-alleles of wild Malus species were amplified, sequenced and compared using polymerase chain reaction (PCR) technology. 21 S-alleles were identified in 27 wild Malus species. The results indicated that the overwhelming majority of S-alleles between wild Malus species and cultivars shared identical sequences. Simultaneously, five new S alleles (designated S48–S52) were identified in wild Malus species. There are the S48-RNase in M. angustifolia (Ation) Michaux, S49-RNase in M. orientalis Uglitzk. Ex Juz. and M. sylvestris (L.) Mill., S50-RNase in M. tschonoskii (Maxim.) C.K. Schneid. and M. sieversii (Ldb.) Roem., S51-RNase in M. komarovii (Sarg.) Rehd. and M. kansuensis (Batal.) C. K. Schneid., S52-RNase in M. manshurica (Maxim.) V. Komorov wild Malus species. Additionally, an S1-RNase was cloned in wild Malus prunifolia var. ringo, which have the same open reading frame as Malus × domestica cv. Fuji, but lacked whole intron.