Plant Molecular Biology Reporter

, Volume 30, Issue 2, pp 453–461

Determination S-Genotypes and Identification of Five Novel S-RNase Alleles in Wild Malus Species

Article

Abstract

Apple (Malus × domestica Borkh.) is a typical Rosaceae species that exhibits gametophytic self-incompatibility (GSI) controlled by polymorphic S-alleles. In this study, the S-alleles of wild Malus species were amplified, sequenced and compared using polymerase chain reaction (PCR) technology. 21 S-alleles were identified in 27 wild Malus species. The results indicated that the overwhelming majority of S-alleles between wild Malus species and cultivars shared identical sequences. Simultaneously, five new S alleles (designated S48S52) were identified in wild Malus species. There are the S48-RNase in M. angustifolia (Ation) Michaux, S49-RNase in M. orientalis Uglitzk. Ex Juz. and M. sylvestris (L.) Mill., S50-RNase in M. tschonoskii (Maxim.) C.K. Schneid. and M. sieversii (Ldb.) Roem., S51-RNase in M. komarovii (Sarg.) Rehd. and M. kansuensis (Batal.) C. K. Schneid., S52-RNase in M. manshurica (Maxim.) V. Komorov wild Malus species. Additionally, an S1-RNase was cloned in wild Malus prunifolia var. ringo, which have the same open reading frame as Malus × domestica cv. Fuji, but lacked whole intron.

Keywords

Determination and identification S-Genotypes S-RNase Wild Malus species 

Supplementary material

11105_2011_345_MOESM1_ESM.doc (54 kb)
ESM 1(DOC 54 kb)

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  1. 1.Laboratory of Fruit Tree Cell and Molecular BreedingChina Agricultural UniversityBeijingChina
  2. 2.Laboratory of Molecular and Developmental Biology, Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijingChina
  3. 3.United Graduate School of Agricultural ScienceIwate UniversityMoriokaJapan

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