Characterization of the LhcSR Gene Under Light and Temperature Stress in the Green Alga Ulva linza
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As a green-tide-forming macroalga, Ulva linza is distributed worldwide and therefore subject to various environmental stresses. The LHCSR (also known as LI818 in green alga and LHCX in diatoms) protein is a stress-related member of the LHC family that plays an important role in photo-protective mechanism, which has been only found in algae. In this study, we cloned full-length cDNA sequence encoding the LhcSR gene from U. linza and analyzed its expression in response to different temperature and illumination gradients. The results showed that high light (HL) could enhance expression of LhcSR and that the expression level peaked at 3 h under HL. Similarly, the expression of LhcSR could also be induced by low temperature (LT). However, the expression patterns of LhcSR were quite different in response to LT and HL treatment. Specifically, the maximum gene expression under LT was much higher (11.8-fold) than under HL (5.4-fold) when compared to the expression under normal conditions. The upregulated expression of LhcSR lasted for 12 h under LT, but 3 h under HL. These data suggest that the LhcSR gene is involved in photoprotection in U. linza, and the results suggest a stronger link to LT. In addition, the discrepancy in expression under HL and LT was consistent with the ecological features of this alga, which only thrives during the cold season (featured as LT and low light).
KeywordsUlva linza LhcSR Full-length Expression analysis Green tide
This work was supported by National special fund for transgenic project (2009ZX08009-019B), the Hi-Tech Research and Development Program (863) of China (2009AA10Z106), Natural Science Foundation of Shandong Province (2009ZRA02075), Qingdao Municipal Science and Technology plan project (09-2-5-8-hy, 10-4-1-13-hy) and the National Science & Technology Pillar Program (2008BAD95B11).
- Fletcher RL (1996) The British Isles. In: Schramm W, Nienhuis PH (eds) Marine benthic vegetation: recent changes and the effects of eutrophication. Springer, Berlin, pp 150–223Google Scholar
- Guiry MD, Guiry GM (2007) Algae Base version 4.2. Worldwide electronic publication: National University of Ireland, Galway. Available at: http://www.algaebase.org (Accessed 2 May 2007).
- Ledford HK, Baroli I, Shin JW, Fischer BB, Eggen RIL, Niyogi KK (2004) Comparative profiling of lipid-soluble antioxidants and transcripts reveals two phases of photo-oxidative stress in a xanthophyll-deficient mutant of Chlamydomonas reinhardtii. Mol Genet Genomics 272:470–479PubMedCrossRefGoogle Scholar
- Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCT method. Methods 25:402–408Google Scholar
- Miura K, Yamano T, Yoshioka S, Kohinata T, Inoue Y, Taniguchi F, Asamizu E, Nakamura Y, Tabata S, Yamato KT, Ohyama K, Fukuzawa H (2004) Expression profiling-based identification of CO2-responsive genes regulated by CCM1 controlling a carbon-concentrating mechanism in Chlamydomonas reinhardtii. Plant Physiol 135:1595–1607PubMedCrossRefGoogle Scholar
- Mu XW, Lu QQ, Hu CM, Chen SY, Zhang T, Zhang XF, Xu P (2010) Preliminary report on the green algae investigation in mariculture ponds along the coastline of Jiangsu Province. In: Wang QY (ed) Marine aquaculture industry based on ecologic system. China Ocean Press, Beijing, pp 28–37Google Scholar
- Nymark M, Valle KC, Brembu T, Hancke K, Winge P, Andresen K, Johnsen G, Bones AM (2009) An integrated analysis of molecular acclimation to high light in the marine diatom Phaeodactylum tricornutum. PloS ONE 4:e7743Google Scholar
- Park S, Jung G, Hwan Y-S, Jin E (2010) Dynamic response of the transcriptome of a psychrophilic diatom, Chaetoceros neogracile, to high irradiance. Planta 231:349–360Google Scholar
- Sambrook J, Fritsch EF, Maniatis T (2001) Molecular cloning: a laboratory manual. Cold Spring Harbour Laboratory Press, New YorkGoogle Scholar
- Ye NH, Zhang XW, Mao YZ, Liang CW, Xu D, Zou J, Zhuang ZM, Wang QY (2011) ‘Green tides’ are overwhelming the coastline of our blue planet: taking the world’s largest example. Ecol Res. doi: 10.1007/s11284-011-0821-8
- Ye NH, Zhuang ZM, Jin XS, Wang QY, Zhang XW, Li DM, Wang HX, Mao YZ, Jiang ZJ, Li B, Xue ZX (2008) China is on the tracking Enteromorpha spp Forming green tide. Nature Proceedings hdl:10101/npre 2008; 2352.1.Google Scholar