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Plant Molecular Biology Reporter

, Volume 28, Issue 2, pp 285–291 | Cite as

Polymorphic Microsatellite Markers Transferable Across Capsicum Species

  • Ayse Gul Ince
  • Mehmet KaracaEmail author
  • Ahmet Naci Onus
Article

Abstract

Pepper (Capsicum annuum L.) is one of the most important crops in the family Solanaceae. However, the number of polymorphic molecular loci detected in this important crop is far behind that of other cultivated plant species. In the present study, a total of 45 microsatellite primer pairs were developed using Capsicum expressed sequence tags databases. Microsatellite primer pairs were tested using several species of Capsicum and several genera in the family Solanaceae including tomato, potato, eggplant, and tobacco. Results indicated that microsatellite primer pairs amplified genomic targets of C. annuum L., Capsicum baccatum L., Capsicum chacoense L., Capsicum chinense L., Capsicum frutescens L., and Capsicum pubescens Ruiz et Pavon, indicating species transferability within Capsicum. Further analyses revealed that amplicons of these primer pairs segregated 1:2:1 or 3:1 Mendelian fashions in 38 F2 individuals of pepper. It was also noted that markers derived from sequences containing dinucleotide repeats were generally more polymorphic at the intraspecific level than sequences containing trinucleotide repeats. All the microsatellite primer pairs developed in this study will be useful for marker-assisted selection and mapping studies in pepper.

Keywords

EST-microsatellites Cross-amplification Touch-down PCR 

Abbreviations

EST

Expressed sequence tag

SSRs

Simple sequence repeats

Td-PCRs

Touch-down polymerase chain reactions

TRA 1.5

Tandem Repeats Analyzer 1.5

Notes

Acknowledgments

This work was supported in part by the Scientific and Technological Research Council and Akdeniz University Coordination Unit of Scientific Research Projects. The authors thank two anonymous referees for contributing valuable information to this manuscript.

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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Ayse Gul Ince
    • 1
  • Mehmet Karaca
    • 1
    Email author
  • Ahmet Naci Onus
    • 1
  1. 1.Faculty of Agriculture, Department of Field CropsAkdeniz UniversityAntalyaTurkey

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