Polymorphic Microsatellite Markers Transferable Across Capsicum Species
- 405 Downloads
Pepper (Capsicum annuum L.) is one of the most important crops in the family Solanaceae. However, the number of polymorphic molecular loci detected in this important crop is far behind that of other cultivated plant species. In the present study, a total of 45 microsatellite primer pairs were developed using Capsicum expressed sequence tags databases. Microsatellite primer pairs were tested using several species of Capsicum and several genera in the family Solanaceae including tomato, potato, eggplant, and tobacco. Results indicated that microsatellite primer pairs amplified genomic targets of C. annuum L., Capsicum baccatum L., Capsicum chacoense L., Capsicum chinense L., Capsicum frutescens L., and Capsicum pubescens Ruiz et Pavon, indicating species transferability within Capsicum. Further analyses revealed that amplicons of these primer pairs segregated 1:2:1 or 3:1 Mendelian fashions in 38 F2 individuals of pepper. It was also noted that markers derived from sequences containing dinucleotide repeats were generally more polymorphic at the intraspecific level than sequences containing trinucleotide repeats. All the microsatellite primer pairs developed in this study will be useful for marker-assisted selection and mapping studies in pepper.
KeywordsEST-microsatellites Cross-amplification Touch-down PCR
Expressed sequence tag
Simple sequence repeats
Touch-down polymerase chain reactions
- TRA 1.5
Tandem Repeats Analyzer 1.5
This work was supported in part by the Scientific and Technological Research Council and Akdeniz University Coordination Unit of Scientific Research Projects. The authors thank two anonymous referees for contributing valuable information to this manuscript.
- Anonymous (2007) Food and Agriculture Organization of the United Nations. Available via FAOSTAT@fao.orgGoogle Scholar
- Mueller LA, Solow TH, Taylor N, Skwarecki B, Buels R, Binns J, Lin C, Wright MH, Ahrens R, Wang Y, Herbst EV, Keyder ER, Menda N, Zamir D, Tanksley SD (2005) The SOL Genomics Network: a comparative resource for Solanaceae biology and beyond. Plant Physiol 138:1310–1317. doi: 10.1104/pp. 105.060707 CrossRefPubMedGoogle Scholar
- Rozen S, Skaletsky HJ (2000) Primer3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics methods and protocols: methods in molecular biology. Humana Press, Totowa, pp 365–386Google Scholar
- Wu F, Mueller LA, Crouzillat D, Pe´tiard V, Tanksley SD (2006) Combining bioinformatics and phylogenetics to identify large sets of single-copy orthologous genes (COSII) for comparative, evolutionary and systematic studies: a test case in the Euasterid plant clade. Genetics 174:1407–1420. doi: 10.1534/genetics.106.062455 CrossRefPubMedGoogle Scholar