Molecular Cloning and Characterization of Rubber Biosynthetic Genes from Taraxacum koksaghyz
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Rubber biosynthesis requires the action of specific enzymes known as cis-prenyltransferases (CPTs). These enzymes are responsible for the sequential addition of isopentenyl pyrophosphate units to the growing polyisoprene chain, a biochemical reaction thought to be stimulated by the presence of small rubber particle proteins (SRPPs). We have cloned, characterized, and analyzed the expression of three CPT genes (TkCPT1–3) and five SRPP genes (TkSRPP1–5) from the rubber-producing plant Taraxacum koksaghyz. The deduced TkCPT amino acid sequences showed significant levels of sequence identity with Hevea brasiliensis CPTs. We also found no obvious differences between SRPPs from T. koksaghyz, another rubber producer, and a non-rubber plant. The roles of the individual TkCPTs and TkSRRPs in rubber biosynthesis are discussed.
KeywordsTaraxacum koksaghyz Rubber Latex Laticifers Rubber particles
- CPT cis
- TPT trans
small rubber particle proteins
rubber elongation factor
The technical assistance of Sandra Ponanta is gratefully acknowledged. This work was supported by grants from the Ministry of Science and Education of Germany (grant no. FKZ 0313712) and Evonik Industries.
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