A Maize Early Responsive to Dehydration Gene, ZmERD4, Provides Enhanced Drought and Salt Tolerance in Arabidopsis

Article

Abstract

Early responsive to dehydration (ERD) genes could be rapidly induced to respond to dehydration and to various other abiotic stresses. Here, we report on an ERD gene (ZmERD4) from maize cloned by rapid amplification of complementary DNA (cDNA) ends. The ZmERD4 cDNA had a total length of 2,536 bp with an open reading frame of 2,196 bp, 5′-untranslated region (UTR) of 48 bp, and 3′-UTR of 292 bp. The gene encoded a predicted polypeptide of 732 amino acids. The ZmERD4 protein shared a high amino acid sequence similarity with ERD4 of Oryza sativa and Arabidopsis thaliana. A reverse-transcription polymerase chain reaction analysis revealed that ZmERD4 was constitutively expressed in different tissues. RNA gel blot showed that ZmERD4 could be induced by both drought and salt stress and also responded to abscisic acid treatment, but it was not induced by low temperature (4°C). Transgenic Arabidopsis plants constitutively expressing the ZmERD4 gene under the control of the 35S cauliflower mosaic virus 35S promoter exhibited slightly smaller-sized leaves under normal growing conditions. Moreover, 35S::ZmERD4 transgenic plants displayed enhanced tolerance to water deficit and high salinity when compared to wild-type plants. Altogether, these findings suggested that ZmERD4 played an important role in early stages of plant adaptation to stress conditions and might be useful in improving plant tolerance to abiotic stress.

Keywords

ZmERD4 Stress Gene expression Maize Transgenic Arabidopsis 

Abbreviations

ERD

early responsive to dehydration

ABA

abscisic acid

RT-PCR

reverse-transcription polymerase chain reaction

ORF

open reading frame

CaMV 35S

cauliflower mosaic virus 35S promoter

Notes

Acknowledgements

The research was supported by the 973 project (grant no. 2006CB101700), the 863 project (grant no. 20060110Z1141), and the National Natural Science Foundation (grant no. 30571133).

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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  1. 1.Institute of Crop Science, Chinese Academy of Agricultural Sciences/The National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI)BeijingChina
  2. 2.Hebei North UniversityZhangjiakouChina
  3. 3.The Cereal Crops InstituteHenan Academy of Agricultural SciencesZhengzhouChina

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