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Plant Molecular Biology Reporter

, Volume 27, Issue 2, pp 126–131 | Cite as

Development of Chromosome Segment Substitution Lines Derived from Backcross between Two Sequenced Rice Cultivars, Indica Recipient 93-11 and Japonica Donor Nipponbare

  • Wenyin Zhu
  • Jing Lin
  • Dewei Yang
  • Ling Zhao
  • Yadong Zhang
  • Zhen Zhu
  • Tao Chen
  • Cailin WangEmail author
Article

Abstract

Chromosome segment substitution lines (CSSLs) are powerful tools for detecting and precisely mapping quantitative trait loci (QTLs) and evaluating gene action as a single factor. In this study, 103 CSSLs were produced using two sequenced rice cultivars: 93-11, an elite restorer indica cultivar as recipient, and Nipponbare, a japonica cultivar, as donor. Each CSSL carried a single chromosome substituted segment. The total length of the substituted segments in the CSSLs was 2,590.6 cM, which was 1.7 times of the rice genome. To evaluate the potential application of these CSSLs for QTL detection, phenotypic variations of seed shattering, grain length and grain width in 10 CSSLs were observed. Two QTLs for seed shattering and three for grain length and grain width were identified and mapped on rice chromosomes. The results demonstrate that CSSLs are excellent genetic materials for dissecting complex traits into a set of monogenic loci. These CSSLs are of great potential value for QTL mapping and plant marker-assisted breeding (MAB).

Keywords

Chromosome segment substitution lines Quantitative trait loci Rice SSR marker 

Abbreviations

CSSLs

Chromosome segment substitution lines

MAB

Marker-assisted breeding

MAS

Marker-assisted selection

QTLs

Quantitative trait loci

SSR

Simple sequence repeats

Notes

Acknowledgements

These studies were funded by National Science and Technology Program (2006BAD01A01), Major Research Program of Agricultural Structure Adjustment (05-01-05B) and Jiangsu High Technology Program (BG2004304, BG2005301).

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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Wenyin Zhu
    • 1
  • Jing Lin
    • 1
  • Dewei Yang
    • 1
  • Ling Zhao
    • 1
  • Yadong Zhang
    • 1
  • Zhen Zhu
    • 1
  • Tao Chen
    • 1
  • Cailin Wang
    • 1
    Email author
  1. 1.Institute of Food Crops, Jiangsu High Quality Rice Research and Development CenterJiangsu Academy of Agricultural SciencesNanjingPeople’s Republic of China

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