Semi-automated, Membrane-Based Protocol for DNA Isolation from Plants

  • Natalia V. Ivanova
  • Aron J. Fazekas
  • Paul D. N. Hebert
Article

Abstract

Many plant species are considered difficult for DNA isolation due to their high concentrations of secondary metabolites such as polysaccharides and polyphenols. Several protocols have been developed to overcome this problem, but they are typically time-consuming, not scalable for high throughput and not compatible with automation. Although a variety of commercial kits are available for plant DNA isolation, their cost is high and these kits usually have limited taxonomic applicability. In a previous study we developed an inexpensive automation-friendly protocol for DNA extraction from animal tissues. Here we demonstrate that a similar protocol allows DNA isolation from plants.

Keywords

DNA isolation Glass fiber plates Plants Automation DNA barcoding 

Abbreviations

CTAB

cetyltrimethylammonium bromide

PVP

polyvinylpyrrolidone

ILB

insect lysis buffer

GuSCN

guanidine thiocyanate

SDS

sodium dodecyl sulfate

Notes

Acknowledgements

This work was supported by grants to PDNH from Genome Canada through the Ontario Genomics Institute, the Canada Foundation for Innovation, the Ontario Innovation Trust, the Canada Research Chairs Program and NSERC. We thank M. Hajibabaei for comments on the manuscript, J. Gerrath for assistance in the field and I. Meusier for evaluation of the automated protocol.

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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Natalia V. Ivanova
    • 1
  • Aron J. Fazekas
    • 2
  • Paul D. N. Hebert
    • 1
  1. 1.Canadian Centre for DNA Barcoding, Biodiversity Institute of OntarioUniversity of GuelphGuelphCanada
  2. 2.Department of Integrative BiologyUniversity of GuelphGuelphCanada

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