Plant Molecular Biology Reporter

, Volume 26, Issue 1, pp 1–11 | Cite as

Ri-mediated Transformation of Glycyrrhiza uralensis with a Squalene Synthase Gene (GuSQS1) for Production of Glycyrrhizin

  • Hong-Yu Lu
  • Jing-Mei Liu
  • Hai-Chao Zhang
  • Tao Yin
  • Shan-Lin Gao
Article

Abstract

Root of Glycyrrhiza uralensis, one of the most important medicinal plants, containing bioactive triterpene saponins (glycyrrhizin). Squalene synthase (SQS) plays a regulatory role in the biosynthesis of triterpene saponins. In the present investigation, SQS coding sequence from G. uralensis was cloned by polymerase chain reaction (PCR) and a transgenic system was developed for G. uralensis through Agrobacterium rhizogenes-mediated transformation. The SQS gene placed under a CaMV 35S promoter was transferred into G. uralensis using A. rhizogenes strain ACCC10060. The transformed hairy roots were selected on Murashige and Skoog (1962)-containing phosphinothricin (PPT) and root lines were established. The integration of SQS gene was confirmed by PCR and Southern blot. Three transgenic root lines UP1, UP24, UP31 were obtained and their growth rates were detected. The result showed that transgenic root lines but UP1 line grew faster than control hairy roots; high-performance liquid chromatography (HPLC) analysis demonstrated the highest glycyrrhizin content of transgenic roots was 2.5 mg/g dry weight and was about 2.6 times higher than control hairy roots.

Keywords

Glycyrrhizin Squalene synthase (SQS) Glycyrrhiza uralensis Hairy roots 

Abbreviations

SQS

squalene synthase

 

β-amyrin synthase

OSC

oxido- squalene cyclase

GuSQS

Glycyrrhiza uralensis squalene synthase

PPT

phosphinothricin

GgSQS

Glycyrrhiza glabra squalene synthase

AS

acetosyringone

Kana

kanamycin sulphate

cef

cefotaxim sodium

cb

carbenicillin

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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Hong-Yu Lu
    • 1
  • Jing-Mei Liu
    • 2
  • Hai-Chao Zhang
    • 1
  • Tao Yin
    • 3
  • Shan-Lin Gao
    • 1
  1. 1.Department of Breeding and GeneticsChina Pharmaceutical UniversityNanjingChina
  2. 2.Chinese Academy of Agricultural SciencesBeijingChina
  3. 3.College of Agriculture and BiotechnologyZhejiang UniversityHangzhouChina

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