Contrasting effects of light, soil chemistry and phylogeny on leaf nutrient concentrations in cave-dwelling plants
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Background and aims
The drivers of variations in leaf nutrient concentrations in cave-dwelling plants remain poorly understood. We aimed to explore the effects of light, soil chemistry and phylogeny on leaf nutrient concentrations in cave-dwelling plants.
We quantified light availability and sampled top-soils and leaves of the co-existing herbs and ferns in three caves. We used the traditional and phylogenetic comparative methods to determine the effects of light, soil chemistry and phylogeny on leaf nutrient concentrations and the cross-species correlations between leaf nutrients.
Leaf nutrient concentrations differed little among caves due to the non-significant relationships of leaf nutrient concentrations with light availability and soil nutrient concentrations across caves. The phylogenetic signals in leaf nutrient concentrations were significant for Ca, Mg and N but non-significant for the remaining nutrients. The evolutionary rates of leaf nutrient concentrations tended to increase with decreasing phylogenetic signals and were faster in herbs than ferns. These contrasting degrees of phylogenetic conservatism in leaf nutrient concentrations were best generated by Ornstein-Uhlenbeck models, i.e., stabilizing selection towards an optimum across species for P, K, S, Fe, Mn and Zn or higher optimal concentrations in herbs than ferns for Ca, Mg and N. Strong cross-species correlations between leaf nutrient concentrations such as Ca vs Mg and N vs P were found.
Leaf nutrient concentrations in cave-dwelling plants showed convergent adaptations to cave environments and presented contrasting degrees of phylogenetic conservatism to produce leaf nutritional diversity for the co-existing herbs and ferns in caves.
KeywordsCave-dwelling plants Leaf nutrient concentrations Phylogenetic conservatism Light Soil chemistry Phylogenetic comparative methods Stabilizing selection
This research was supported by the Guangxi Natural Science Foundation (2013GXNSFBA019079), Guangxi Scientific and Technological Project (1355007-3) and National Natural Science Foundation (31570307; 31860042; 31860174) in China. Special thanks to the anonymous reviewers and the responsible editor (Alfonso Escudero) for providing insightful comments on the early versions of the manuscript.
- Ågren GI (2008) Stoichiometry and nutrition of plant growth in natural communities. Annu Rev Ecol Evol Syst 39:153–170. https://doi.org/10.1146/annurev.ecolsys.39.110707.173515 CrossRefGoogle Scholar
- Clavel J (2018) Multivariate comparative tools for fitting evolutionary models to morphometric data. https://github.com/JClavel/mvMORPH. Accessed 31 July 2018
- de la Riva EG, Villar R, Pérez-Ramos IG, Quero JL, Matías L, Poorter L, Marañón T (2018) Relationships between leaf mass per area and nutrient concentrations in 98 Mediterranean woody species are determined by phylogeny, habitat and leaf habit. Trees 32:497–510. https://doi.org/10.1007/s00468-017-1646-z CrossRefGoogle Scholar
- Hansen TF (1997) Stabilizing selection and the comparative analysis of adaptation. Evolution 51:1341–1351. https://doi.org/10.1111/j.1558-5646.1997.tb01457.x CrossRefPubMedGoogle Scholar
- R Development Core Team (2008) R: a language and environment for statistical computing. R Foundation for Statistical Computing, Vienna http://www.R-project.org. Accessed 2018-07-02
- Sardans J, Janssens IA, Alonso R, Veresoglou SD, Rillig G, Peñuelas J (2015) Foliar elemental composition of European forest tree species associated with evolutionary traits and present environmental and competitive conditions. Glob Ecol Biogeogr 24:240–255. https://doi.org/10.1111/geb.12253 CrossRefGoogle Scholar
- Stein RJ, Höreth S, de Melo JRF, Syllwasschy L, Lee G, Garbin M (2017) Relationships between soil and leaf mineral composition are element-specific, environmental-dependent and geographically structured in the emerging model Arabidopsis halleri. New Phytol 213:1274–1286. https://doi.org/10.1111/nph.14219 CrossRefPubMedGoogle Scholar
- Tosens T, Nishida K, Gago J, Coopman RE, Cabrera HM, Carriquí M, Laanisto L, Morales L, Nadal M, Rojas R, Talts E, Tomas M, Hanba Y, Niinemets Ü, Flexas J (2016) The photosynthetic capacity in 35 ferns and fern allies: mesophyll CO2 diffusion as a key trait. New Phytol 209:1576–1590. https://doi.org/10.1111/nph.13719 CrossRefPubMedGoogle Scholar
- White PJ, Broadley MR, Thompson JA, McNicol JW, Crawley MJ, Poulton PR, Jonston AE (2012) Testing the distinctness of shoot ionomes of angiosperm families using the Rothamsted Park grass continuous Hay experiment. New Phytol 196:101–109. https://doi.org/10.1111/j.1469-8137.2012.04228.x CrossRefPubMedGoogle Scholar