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Interactions between putatively endophytic bacteria and tall fescue (Festuca arundinacea): plant growth promotion and colonization in host and non-host cultivars

  • Patricia Vaz Jauri
  • Cecilia Taulé
  • Maria Cecilia de los Santos
  • Belén Fernandez
  • Andrés Di Paolo
  • José Sotelo
  • Federico BattistoniEmail author
Regular Article
  • 103 Downloads

Abstract

Aims

Tall fescue (Festuca arundinacea) is a forage grass highly used in prairies. Cultivar SFRO Don Tomás has excellent drought and cold resistance, characteristics which are influenced by the native endophytic bacterial community. The aims of this work were to deepen the characterization of a selected group of putatively endophytic strains with different plant growth promoting (PGP) traits and to analyze the relationship between colonization and PGP abilities.

Methods

PGP with single inoculations in pots containing a mixture of sterile sand and field soil and with paired inoculations in field soil cores were carried out. Colonization of plants inoculated singly or co-inoculated under gnotobiotic conditions was quantified. Confocal laser microscopy was used to visualize the colonization and infection processes of strain Streptomyces sp. UYFA156 in cultivars SFRO Don Tomás and Tacuabé.

Results

Tall fescue cultivars displayed different responses to single bacterial inoculation. Inoculation with two strain pairs which included UYFA156 were successful in promoting growth in soil cores. Higher bacterial densities did not correspond to higher PGP. Strain UYFA156 was observed colonizing both fescue cultivars.

Conclusions

UYFA156 is a true endophyte of tall fescue. Its PGP activity is cultivar-specific and may be related to the modulation of other endophytes.

Keywords

Tall fescue Plant growth promotion Bacterial endophytes Endophytic Streptomyces 

Notes

Acknowledgements

This work was supported by grants from the Program Alianza para el Desarrollo (Project ALI-1-3269) of the Uruguayan National Agency for Innovation and Research (ANII), the Uruguayan Program for the Development of Basic Sciences (PEDECIBA), and the International Centre for Genetical Engineering and Biotechnology (ICGEB, Project CRP/URY17_01). The authors are very grateful to the member of the Rural Promotion Society Ortiz (SFRO) Ing. José Mesa and Dr. Paola Scavone for her assistance in microscopy techniques.

Supplementary material

11104_2019_4359_MOESM1_ESM.docx (1.7 mb)
ESM 1 (DOCX 1712 kb)

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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Patricia Vaz Jauri
    • 1
  • Cecilia Taulé
    • 1
  • Maria Cecilia de los Santos
    • 1
  • Belén Fernandez
    • 1
  • Andrés Di Paolo
    • 2
  • José Sotelo
    • 2
  • Federico Battistoni
    • 1
    Email author
  1. 1.Microbial Biochemistry and Genomics DepartmentClemente Estable Biological Research InstituteMontevideoUruguay
  2. 2.Genomic DepartmentClemente Estable Biological Research InstituteMontevideoUruguay

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