Interactions between putatively endophytic bacteria and tall fescue (Festuca arundinacea): plant growth promotion and colonization in host and non-host cultivars
- 103 Downloads
Tall fescue (Festuca arundinacea) is a forage grass highly used in prairies. Cultivar SFRO Don Tomás has excellent drought and cold resistance, characteristics which are influenced by the native endophytic bacterial community. The aims of this work were to deepen the characterization of a selected group of putatively endophytic strains with different plant growth promoting (PGP) traits and to analyze the relationship between colonization and PGP abilities.
PGP with single inoculations in pots containing a mixture of sterile sand and field soil and with paired inoculations in field soil cores were carried out. Colonization of plants inoculated singly or co-inoculated under gnotobiotic conditions was quantified. Confocal laser microscopy was used to visualize the colonization and infection processes of strain Streptomyces sp. UYFA156 in cultivars SFRO Don Tomás and Tacuabé.
Tall fescue cultivars displayed different responses to single bacterial inoculation. Inoculation with two strain pairs which included UYFA156 were successful in promoting growth in soil cores. Higher bacterial densities did not correspond to higher PGP. Strain UYFA156 was observed colonizing both fescue cultivars.
UYFA156 is a true endophyte of tall fescue. Its PGP activity is cultivar-specific and may be related to the modulation of other endophytes.
KeywordsTall fescue Plant growth promotion Bacterial endophytes Endophytic Streptomyces
This work was supported by grants from the Program Alianza para el Desarrollo (Project ALI-1-3269) of the Uruguayan National Agency for Innovation and Research (ANII), the Uruguayan Program for the Development of Basic Sciences (PEDECIBA), and the International Centre for Genetical Engineering and Biotechnology (ICGEB, Project CRP/URY17_01). The authors are very grateful to the member of the Rural Promotion Society Ortiz (SFRO) Ing. José Mesa and Dr. Paola Scavone for her assistance in microscopy techniques.
- Baltz RH (2007) Antimicrobials from Actinomycetes: Back to the future. Microbiol 2:125–131Google Scholar
- de los Santos MC, Taulé C, Mareque C et al (2015) Identification and characterization of the part of the bacterial community associated with field-grown tall fescue (Festuca arundinacea) cv. SFRO Don Tomás in Uruguay. Ann Microbiol 66:629–642Google Scholar
- Hardoim PR, van Overbeek LS, Berg G, Pirttilä AM, Compant S, Campisano A, Döring M, Sessitsch A (2015) The hidden world within plants: ecological and evolutionary considerations for defining functioning of microbial Endophytes. Microbiol Mol Biol Rev 79:293–320PubMedPubMedCentralCrossRefGoogle Scholar
- Hernández-Calderón E, Aviles-Garcia ME, Castulo-Rubio DY, Macías-Rodríguez L, Ramírez VM, Santoyo G, López-Bucio J, Valencia-Cantero E (2018) Volatile compounds from beneficial or pathogenic bacteria differentially regulate root exudation, transcription of iron transporters, and defense signaling pathways in Sorghum bicolor. Plant Mol Biol 96:291–304PubMedCrossRefPubMedCentralGoogle Scholar
- Kieser T, John Innes Foundation (2000) Practical Streptomyces genetics. John Innes Foundation, NorwichGoogle Scholar
- Shimizu M, Meguro A, Hasegawa S et al (2006) Disease resistance induced by nonantagonistic endophytic Streptomyces spp . on tissue-cultured seedlings of rhododendron. Annu Rev Phytopathol 72:351–354Google Scholar
- Tarkka M, Hampp R (2008) Secondary metabolites of soil Streptomycetes in biotic interactions. In: secondary metabolites in soil ecology. Soil Biology, p 107Google Scholar
- Vincent JM (1970) A manual for the practical study of root-nodule bacteria. Blackwell Scientific, OxfordGoogle Scholar
- Zhalnina K, Louie KB, Hao Z, Mansoori N, da Rocha UN, Shi S, Cho H, Karaoz U, Loqué D, Bowen BP, Firestone MK, Northen TR, Brodie EL (2018) Dynamic root exudate chemistry and microbial substrate preferences drive patterns in rhizosphere microbial community assembly. Nat Microbiol 3:470–480PubMedCrossRefGoogle Scholar