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Differences in ectomycorrhizal community assembly between native and exotic pines are reflected in their enzymatic functional capacities

  • Chen NingEmail author
  • Wenhua Xiang
  • Gregory M. Mueller
  • Louise M. Egerton-Warburton
  • Wende Yan
  • Shuguang Liu
Regular Article

Abstract

Background and Aims

Introducing exotic tree species for afforestation out of their natural range may alter the local ectomycorrhizal (ECM) fungal communities. The potential functional consequences shaped by exotic trees with recruited local ECM fungi rather than native trees remain unclear. This study examined (a) whether the composition and extracellular enzyme function of ECM fungal communities differed between native masson pine (Pinus massoniana) and exotic slash pine (Pinus elliottii) during seedling establishment; and (b) how differences in enzyme functioning were linked to the growth pattern of the host plants.

Methods

Native (masson) and exotic (slash) pine seedlings were planted into soil cores collected from each study site. At three months growth, root tips were collected from seedlings and assayed for ECM fungal community composition using high-throughput sequencing, and functioning using single root tip assays for enzymes associated with N, P and C acquisition.

Results

ECM fungi on masson pines showed higher activities of nitrogen- (N-acetylglucosaminidase, 280–300%), phosphorus- (acid phosphatase, 105–152%), and cellulose (β-glucosidase, 204–235%; cellobiohydrolase, 142–255%) compound degrading enzymes compared to those on slash pines. Those differences was attributed to the host-specific performance of certain ECM fungal taxa, such as Rhizopogon spp. Information theory model selection showed that plant nutrient status in masson pines was correlated with the enzymatic contribution of Rhizopogon spp., whereas slash pines depended on a diverse enzyme palette from multiple ECM fungal taxa.

Conclusions

Host identity strongly influenced ECM fungal community composition and extracellular enzymatic functions of specific ECM fungal taxa, which could feedback to host establishment and nutrient cycling processes of restored ecosystem. Therefore, the origin of afforestation tree species should be an important factor when selecting tree species for restoration of degraded lands.

Keywords

Community structure and function Ectomycorrhizal fungi Extracellular enzyme Exotic pine Rhizopogon Russula 

Notes

Acknowledgments

We thank the graduate students Xiaowei Ni, Zhizhou Liu, Yi Chen, Xinghao Huang, and Juan Cao for their assistance in the field and laboratory manipulations. We thank the Forest Administration of of Longli Forest Farm for the permission to sample and carry out this field investigation. We also thank Dr. Peter Avis and Dr. Andrew Wilson for their comments on an earlier version of the paper. This study was supported by National Science Foundation of China (31570447, awarded to WX), the collaborative program of Plant Biology and Conservation at Northwestern University and Chicago Botanic Garden, and China Scholarship Council (CSC201408430072, awarded to CN). We gratefully acknowledge Dr. Martin Nuñez and two anonymous reviewers for providing invaluable comments during the review of this manuscript.

Author’s contributions

CN, GMM and LEW conceived the ideas and designed methodology; CN collected the data; CN analyzed the data; CN, WX, LEW, and GMM led the writing of the manuscript. All authors contributed critically to the drafts and gave final approval for publication.

Supplementary material

11104_2019_4355_MOESM1_ESM.docx (339 kb)
ESM 1 (DOCX 339 kb)

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© Springer Nature Switzerland AG 2019

Authors and Affiliations

  1. 1.Faculty of Life Science and TechnologyCentral South University of Forestry and TechnologyChangshaChina
  2. 2.Program in Plant Biology and ConservationNorthwestern UniversityEvanstonUSA
  3. 3.Chicago Botanic GardenGlencoeUSA

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