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Plant and Soil

, Volume 443, Issue 1–2, pp 429–447 | Cite as

Colonization of Arabidopsis thaliana by Herbaspirillum seropedicae promotes its growth and changes its proteomic profile

  • Mariana Ramos Leandro
  • Patrícia Louzada Rangel
  • Tamires Cruz dos Santos
  • Leandro Fernandes Andrade
  • Luciano de Souza Vespoli
  • Ana Lidia Soares Rangel
  • Suzane Ariadina de Souza
  • Roberta Ribeiro Barbosa
  • Lucas Zanchetta Passamani
  • Vanildo Silveira
  • Gonçalo Apolinário de Souza FilhoEmail author
Regular Article
  • 172 Downloads

Abstract

Background and aims

Herbaspirillum seropedicae is a Plant Growth Promoting Bacterium (PGPB) that colonizes crops of economic interest, especially grasses. Here, we aimed to characterize the association of H. seropedicae with the model dicot Arabidopsis thaliana and its effect on plant growth. The molecular pathways activated during the association were monitored by comparative proteomics.

Methods

Arabidopsis thaliana seedlings were inoculated with H. seropedicae and grown for 28 days. Plant growth promotion was analyzed by quantifying the total leaf area and the fresh and dry biomass. The endophytic bacterial population was quantified by counts of colony-forming units (CFU), and the bacterial location in plant tissues was determined by epifluorescence microscopy. Changes in the proteomic profile of the plants were investigated by ESI-LC-MS/MS.

Results

Herbaspirillum seropedicae colonized the roots of A. thaliana, but was absent from the shoots. The association promoted plant growth significantly. The proteomic analysis revealed several protein groups regulated in the roots and shoots during the association, with an emphasis on the photosynthesis and phenylpropanoid biosynthesis.

Conclusions

Herbaspirillum seropedicae colonizes A. thaliana roots and promotes its growth, leading to an increase in biomass and the modulation of specific proteomic responses. These findings open new perspectives into the study of the molecular basis of the positive interaction between H. seropedicae and host plants, both monocots and dicots.

Keywords

PGPB Beneficial bacteria Plant-bacteria Endophytic bacteria Biological nitrogen fixation 

Notes

Supplementary material

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Figure S1 (DOCX 194 kb)
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Figure S2 (DOCX 163 kb)
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Table S1 (DOCX 205 kb)
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Table S2 (DOCX 390 kb)
11104_2019_4236_MOESM5_ESM.xlsx (11 kb)
Table S3 (XLSX 10 kb)

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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Mariana Ramos Leandro
    • 1
  • Patrícia Louzada Rangel
    • 1
  • Tamires Cruz dos Santos
    • 1
  • Leandro Fernandes Andrade
    • 1
  • Luciano de Souza Vespoli
    • 1
  • Ana Lidia Soares Rangel
    • 1
  • Suzane Ariadina de Souza
    • 1
  • Roberta Ribeiro Barbosa
    • 1
  • Lucas Zanchetta Passamani
    • 1
  • Vanildo Silveira
    • 1
  • Gonçalo Apolinário de Souza Filho
    • 1
    Email author
  1. 1.Laboratório de Biotecnologia (Setor de Biologia Integrativa)Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF)Rio de JaneiroBrazil

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