Interactive effects of nitrogen and phosphorus additions on plant growth vary with ecosystem type
Co-limitation of ecosystem productivity by nitrogen (N) and phosphorus (P) is gaining increasing recognition, but how co-limitation through N and P interactions differs among different terrestrial ecosystems remains unclear.
We performed a meta-analysis of 133 independent studies conducted in four natural terrestrial ecosystems to examine the interactive effects of N and P additions on ten plant growth-related variables.
Adding N and P individually or in combination significantly increased aboveground biomass (AGB), and the interactions were uniformly synergistic for AGB, and additive for belowground biomass (BGB), but variable for other eight growth-related variables among four different ecosystems. The interaction was synergistic for leaf P and soil NO3-N only in tropical forests, and antagonistic for soil available P (AP) in tropical forests, leaf N in grasslands, root P in wetlands, and leaf P and soil NH4-N in tundra. The interaction for leaf N: P ratios was additive only in tropical forests, and synergistic in the other three ecosystems.
Our results highlighted the interactions of N and P additions can promote uptake of both nutrients by plants, and plants tend to maintain the optimal nutrient balance for growth and reproduction through regulating biomass production and tissue nutrient concentrations.
KeywordsInteractions Meta-analysis Nutrient limitation Plant growth Terrestrial ecosystems
We are grateful to all the researchers for their data used in this meta-analysis. Thanks are due to the subject editor and three anonymous reviewers for their constructive suggestions on improving the manuscript. This study was financially supported by the National Science Fund for Distinguished Young Scholars (41825020).
J J, Y-P W and J Y designed the research; J J, Y Y and M Y collected the data; J J and C W performed the analysis; J J, Y Y and Y-P W wrote the manuscript.
Compliance with ethical standards
Conflict of interest
The authors declare that no conflict of interest exits in the submission of this manuscript, and the manuscript is approved by all authors for publication.
- Aerts R, Chapin FS III (2000) The mineral nutrition of wild plants revisited: a re-evaluation of processes and patterns. Adv Ecol Res 30:1–67Google Scholar
- Avolio ML, Koerner SE, La Pierre KJ, Wilcox KR, Wilson GWT, Smith MD, Collins SL, MacDougall A (2014) Changes in plant community composition, not diversity, during a decade of nitrogen and phosphorus additions drive above-ground productivity in a tallgrass prairie. J Ecol 102:1649–1660CrossRefGoogle Scholar
- Fay PA, Prober SM, Harpole WS, Knops JM, Bakker JD, Borer ET, Lind EM, MacDougall AS, Seabloom EW, Wragg PD, Adler PB, Blumenthal DM, Buckley YM, Chu C, Cleland EE, Collins SL, Davies KF, Du G, Feng X, Firn J, Gruner DS, Hagenah N, Hautier Y, Heckman RW, Jin VL, Kirkman KP, Klein J, Ladwig LM, Li Q, McCulley RL, Melbourne BA, Mitchell CE, Moore JL, Morgan JW, Risch AC, Schutz M, Stevens CJ, Wedin DA, Yang LH (2015) Grassland productivity limited by multiple nutrients. Nat Plants 1:15080CrossRefGoogle Scholar
- Hooper DU, Johnson L (1999) Nitrogen limitation in dryland ecosystems: responses to geographical and temporal variation in precipitation. Biogeochemistry 46:247–293Google Scholar
- Huang W, Houlton BZ, Marklein AR, Liu J, Zhou G (2015) Plant stoichiometric responses to elevated CO2 vary with nitrogen and phosphorus inputs: evidence from a global-scale meta-analysis. Sci Rep 5Google Scholar
- Lamarque JF, Dentener F, McConnell J, Ro CU, Shaw M, Vet R, Bergmann D, Cameron-Smith P, Dalsoren S, Doherty R, Faluvegi G, Ghan SJ, Josse B, Lee YH, MacKenzie IA, Plummer D, Shindell DT, Skeie RB, Stevenson DS, Strode S, Zeng G, Curran M, Dahl-Jensen D, Das S, Fritzsche D, Nolan M (2013) Multi-model mean nitrogen and sulfur deposition from the atmospheric chemistry and climate model Intercomparison project (ACCMIP): evaluation of historical and projected future changes. Atmos Chem Phys 13:7997–8018CrossRefGoogle Scholar
- Mori T, Ohta S, Ishizuka S, Konda R, Wicaksono A, Heriyanto J, Hamotani Y, Gobara Y, Kawabata C, Kuwashima K (2013) Soil greenhouse gas fluxes and C stocks as affected by phosphorus addition in a newly established Acacia mangium plantation in Indonesia. Forest Ecol Manag 310:643–651CrossRefGoogle Scholar
- Peñuelas J, Poulter B, Sardans J, Ciais P, van der Velde M, Bopp L, Boucher O, Godderis Y, Hinsinger P, Llusia J, Nardin E, Vicca S, Obersteiner M, Janssens IA (2013) Human-induced nitrogen-phosphorus imbalances alter natural and managed ecosystems across the globe. Nat Commun 4:2934CrossRefGoogle Scholar
- Reich PB, Oleksyn J, Wright IJ, Niklas KJ, Hedin L, Elser JJ (2010) Consistent 2 / 3-power leaf nitrogen to phosphorus scaling among major plant groups and biomes. In: Proceedings of the Royal Society of London. Series B: biological sciences, vol 277, pp 877–883Google Scholar
- Rosenberg MS, Adams DC, Gurevitch J (2000) Meta Win: Statistical Software for Meta-Analysis, Version 2. Sinauer Associates, Sunderland, MAGoogle Scholar
- Sterner RW, Elser JJ (2002) Ecological stoichiometry: the biology of elements from molecules to the biosphere. Princeton, NJ. Princeton University Press, USAGoogle Scholar