Plant and Soil

, Volume 440, Issue 1–2, pp 341–356 | Cite as

Soil biota, carbon cycling and crop plant biomass responses to biochar in a temperate mesocosm experiment

  • Sarah A. McCormack
  • Nick Ostle
  • Richard D. Bardgett
  • David W. Hopkins
  • M. Glória Pereira
  • Adam J. VanbergenEmail author
Regular Article


Background and aims

Biochar addition to soil is a carbon capture and storage option with potential to mitigate rising atmospheric CO2 concentrations, yet the consequences for soil organisms and linked ecosystem processes are inconsistent or unknown. We tested biochar impact on soil biodiversity, ecosystem functions, and their interactions, in temperate agricultural soils.


We performed a 27-month factorial experiment to determine effects of biochar, soil texture, and crop species treatments on microbial biomass (PFLA), soil invertebrate density, crop biomass and ecosystem CO2 flux in plant-soil mesocosms.


Overall soil microbial biomass, microarthropod abundance and crop biomass were unaffected by biochar, although there was an increase in fungal-bacterial ratio and a positive relationship between the 16:1ω5 fatty acid marker of AMF mass and collembolan density in the biochar-treated mesocosms. Ecosystem CO2 fluxes were unaffected by biochar, but soil carbon content of biochar-treated mesocosms was significantly lower, signifying a possible movement/loss of biochar or priming effect.


Compared to soil texture and crop type, biochar had minimal impact on soil biota, crop production and carbon cycling. Future research should examine subtler effects of biochar on biotic regulation of ecosystem production and if the apparent robustness to biochar weakens over greater time spans or in combination with other ecological perturbations.


Soil community Charcoal Soil carbon cycling Crop production Ecosystem CO2 flux Biodiversity-function Collembola Mites Nematode AM fungi PLFA 



This research was funded by a Natural Environment Research Council Open CASE PhD studentship grant (NE/HO18085/1). Thanks to Blair McKenzie and Euan Caldwell (James Hutton Institute) and Sean Case (Centre for Ecology and Hydrology) for providing advice and assistance with experimental set-up. Thanks to Adam Butler (Biomathematics and Statistics Scotland) for advice on LMMs. Thanks to Stuart Smith, Emily Taylor, Scott McKenzie, Will Hentley, Albert Johnston and Wilma Johnston for assistance with experiment set-up, maintenance and data collection.

Data statement

Raw data will be archived at the NERC Environmental Information Data Centre Summary data (means + SE) for soil invertebrate densities, above-belowground crop biomass and PFLA are contained in online resources linked to this article (Tables 7S–9S).

Supplementary material

11104_2019_4062_MOESM1_ESM.docx (3.8 mb)
ESM 1 (DOCX 3888 kb)


  1. Ayres E, Wall DH, Bardgett R (2010) Trophic interactions and their implications for soil carbon fluxes. In: Kutsch WL (ed) Soil carbon dynamics, an integrated methodology. Cambridge University Press, Cambridge, UKGoogle Scholar
  2. Backer RGM, Schwinghamer TD, Whalen JK, Seguin P, Smith DL (2016) Crop yield and SOC responses to biochar application were dependent on soil texture and crop type in southern Quebec, Canada. J Plant Nutr Soil Sci 179:399–408. CrossRefGoogle Scholar
  3. Bardgett RD, van der Putten WH (2014) Belowground biodiversity and ecosystem functioning. Nature 515:505–511. CrossRefPubMedGoogle Scholar
  4. Bargmann I, Rillig MC, Buss W, Kruse A, Kuecke M (2013) Hydrochar and biochar effects on germination of spring barley. J Agron Crop Sci 199:360–373. CrossRefGoogle Scholar
  5. Beesley L, Moreno-Jiménez E, Gomez-Eyles JL (2010) Effects of biochar and greenwaste compost amendments on mobility, bioavailability and toxicity of inorganic and organic contaminants in a multi-element polluted soil. Environ Pollut 158:2282–2287CrossRefPubMedGoogle Scholar
  6. Bretherton S, Tordoff GM, Jones TH, Boddy L (2006) Compensatory growth of Phanerochaete velutina mycelial systems grazed by Folsomia candida (Collembola). FEMS Microbiol Ecol 58:33–40CrossRefPubMedGoogle Scholar
  7. Bruun S, Clauson-Kaas S, Bobulska L, Thomsen IK (2014) Carbon dioxide emissions from biochar in soil: role of clay, microorganisms and carbonates. Eur J Soil Sci 65:52–59CrossRefGoogle Scholar
  8. Case S, McNamara N, Reay D, Whitaker J (2012) The effect of biochar addition on N2O and CO2 emissions from a sandy loam soil - the role of soil aeration. Soil Biol Biochem 51:125–134Google Scholar
  9. Chan KY, Xu Z (2009) Biochar: nutrient properties and their enhancement. In: Lehmann J, Joseph S (eds) Biochar for environmental management. Earthscan, LondonGoogle Scholar
  10. Chen G, Qin J, Shi D, Zhang Y, Ji W (2009) Diversity of soil nematodes in areas polluted with heavy metals and polycyclic aromatic hydrocarbons (PAHs) in Lanzhou, China. Environ Manag 44:163–172. CrossRefGoogle Scholar
  11. Chen J, Liu X, Zheng J, Zhang B, Lu H, Chi Z, Pan G, Li L, Zheng J, Zhang X, Wang J, Yu X (2013) Biochar soil amendment increased bacterial but decreased fungal gene abundance with shifts in community structure in a slightly acid rice paddy from Southwest China. Appl Soil Ecol 71:33–44. CrossRefGoogle Scholar
  12. Cross A, Sohi S (2011) The priming potential of biochar products in relation to labile carbon contents and soil organic matter status. Soil Biol Biochem 43:2127–2134CrossRefGoogle Scholar
  13. Domene X, Hanley K, Enders A, Lehmann J (2015) Short-term mesofauna responses to soil additions of corn Stover biochar and the role of microbial biomass. Appl Soil Ecol 89:10–17. CrossRefGoogle Scholar
  14. Dungait JAJ, Ghee C, Rowan JS, McKenzie BM, Hawes C, Dixon ER, Paterson E, Hopkins DW (2013) Microbial responses to the erosional redistribution of soil organic carbon in arable fields. Soil Biol Biochem 60:195–201. CrossRefGoogle Scholar
  15. Dupuy L, Vignes M, McKenzie BM, White PJ (2010) The dynamics of root meristem distribution in the soil. Plant Cell Environ 33:358–369CrossRefPubMedGoogle Scholar
  16. Frostegård Å, Tunlid A, Bååth E (2011) Use and misuse of PLFA measurements in soils. Soil Biol Biochem 43:1621–1625. CrossRefGoogle Scholar
  17. Gebremikael MT, Steel H, Buchan D, Bert W, De Neve S (2016) Nematodes enhance plant growth and nutrient uptake under C and N-rich conditions. Sci Rep 6:32862. CrossRefPubMedPubMedCentralGoogle Scholar
  18. George C, Kohler J, Rillig MC (2016) Biochars reduce infection rates of the root-lesion nematode Pratylenchus penetrans and associated biomass loss in carrot. Soil Biol Biochem 95:11–18. CrossRefGoogle Scholar
  19. Hagner M, Kemppainen R, Jauhiainen L, Tiilikkala K, Setala H (2016) The effects of birch (Betula spp.) biochar and pyrolysis temperature on soil properties and plant growth. Soil Tillage Res 163:224–234. CrossRefGoogle Scholar
  20. Hale SE, Jensen J, Jakob L, Oleszczuk P, Hartnik T, Henriksen T, Okkenhaug G, Martinsen V, Cornelissen G (2013) Short-term effect of the soil amendments activated carbon, biochar, and ferric oxyhydroxide on bacteria and invertebrates. Environ Sci Technol 47:8674–8683. CrossRefPubMedGoogle Scholar
  21. Heemsbergen DA, Berg MP, Loreau M, van Haj JR, Faber JH, Verhoef HA (2004) Biodiversity effects on soil processes explained by interspecific functional dissimilarity. Science 306:1019–1020CrossRefPubMedGoogle Scholar
  22. Hol WHG, Vestergard M, ten Hooven F, Duyts H, van de Voorde TFJ, Bezemer TM (2017) Transient negative biochar effects on plant growth are strongest after microbial species loss. Soil Biol Biochem 115:442–451. CrossRefGoogle Scholar
  23. Jeffery S, Verheijen F, van der Velde M, Bastos AC (2011) A quantitative review of the effects of biochar application to soils on crop productivity using meta-analysis. Agric Ecosyst Environ 144:175–187CrossRefGoogle Scholar
  24. Jenkins JR, Viger M, Arnold EC, Harris ZM, Ventura M, Miglietta F, Girardin C, Edwards RJ, Rumpel C, Fornasier F, Zavalloni C, Tonon G, Alberti G, Taylor G (2017) Biochar alters the soil microbiome and soil function: results of next-generation amplicon sequencing across Europe. GCB Bioenergy 9:591–612. CrossRefGoogle Scholar
  25. Jones DL, Rousk J, Edwards-Jones G, DeLuca TH, Murphy DV (2012) Biochar-mediated changes in soil quality and plant growth in a three year field trial. Soil Biol Biochem 45:113–124CrossRefGoogle Scholar
  26. Lal R (2004) Soil carbon sequestration to mitigate climate change. Geoderma 123:1–22CrossRefGoogle Scholar
  27. Lehmann J (2007) A handful of carbon. Nature 447:143–144CrossRefPubMedGoogle Scholar
  28. Lehmann J, Rondon M (2006) Bio-char soil management on highly-weathered soils in the tropics. In: Uphoff NT (ed) Biological approaches to sustainable soil systems. CRC Press, Boca RatonGoogle Scholar
  29. Lehmann J, Rillig MC, Thies JE, Masiello CA, Hockaday WC, Crowley DC (2011) Biochar effects on soil biota - a review. Soil Biol Biochem 43:1812–1836CrossRefGoogle Scholar
  30. Liang B, Lehmann J, Sohi SP, Thies JE, O'Neill B, Trujillo L, Gaunt J, Solomon D, Grossman J, Neves EG, Luizao FJ (2010) Black carbon affects the cycling of non-black carbon in soil. Org Geochem 41:206–213CrossRefGoogle Scholar
  31. Liu S, Zhang Y, Zong Y, Hu Z, Wu S, Zhou J, Jin Y, Zou J (2016) Response of soil carbon dioxide fluxes, soil organic carbon and microbial biomass carbon to biochar amendment: a meta-analysis. GCB Bioenergy 8:392–406. CrossRefGoogle Scholar
  32. Maestrini B, Nannipieri P, Abiven S (2015) A meta-analysis on pyrogenic organic matter induced priming effect. GCB Bioenergy 7:577–590. CrossRefGoogle Scholar
  33. Major J, Lehmann J, Rondon MA, Goodale C (2010) Fate of soil-applied black carbon: downward migration, leaching and soil respiration. Glob Chang Biol 16:1366–1379CrossRefGoogle Scholar
  34. Marks EAN, Mattana S, Alcaniz JM, Domene X (2014) Biochars provoke diverse soil mesofauna reproductive responses in laboratory bioassays. Eur J Soil Biol 60:104–111CrossRefGoogle Scholar
  35. McCormack S, Ostle NJ, Bardgett R, Hopkins DW, Vanbergen AJ (2013) Biochar in bioenergy cropping systems: impacts on soil faunal communities and linked ecosystem processes. Glob Chang Biol 5:81–95CrossRefGoogle Scholar
  36. McKenzie SW, Johnson SN, Jones H, Ostle NJ, Hails RS, Vanbergen AJ (2016) Root herbivores drive changes to plant primary chemistry, but root loss is mitigated under elevated atmospheric CO2. Front Plant Sci 7.
  37. Ngosong C, Gabriel E, Ruess L (2012) Use of the signature fatty acid 16:1ω5 as a tool to determine the distribution of arbuscular mycorrhizal fungi in soil. J Lipid 2012:236807–236807. CrossRefGoogle Scholar
  38. Nielsen UN, Ayres E, Wall DH, Bardgett RD (2011) Soil biodiversity and carbon cycling: a review and synthesis of studies examining diversity-function relationships. Eur J Soil Sci 62:105–116CrossRefGoogle Scholar
  39. Olsson PA, Bååth E, Jakobsen I, Söderström B (1995) The use of phospholipid and neutral lipid fatty acids to estimate biomass of arbuscular mycorrhizal fungi in soil. Mycol Res 99:623–629. CrossRefGoogle Scholar
  40. Orwin KH, Ostle N, Wilby A, Bardgett RD (2014) Effects of species evenness and dominant species identity on multiple ecosystem functions in model grassland communities. Oecologia 174:979–992. CrossRefPubMedGoogle Scholar
  41. Prayogo C, Jones JE, Baeyens J, Bending GD (2014) Impact of biochar on mineralisation of C and N from soil and willow litter and its relationship with microbial community biomass and structure. Biol Fertil Soils 50:695–702CrossRefGoogle Scholar
  42. Prendergast-Miller MT, Duvall M, Sohi SP (2014) Biochar-root interactions are mediated by biochar nutrient content and impacts on soil nutrient availability. Eur J Soil Sci 65:173–185CrossRefGoogle Scholar
  43. Pressler Y, Foster EJ, Moore JC, Cotrufo MF (2017) Coupled biochar amendment and limited irrigation strategies do not affect a degraded soil food web in a maize agroecosystem, compared to the native grassland. GCB Bioenergy 9:1344–1355. CrossRefGoogle Scholar
  44. Rousk J, Brookes PC, Bååth E (2009) Contrasting soil pH effects on fungal and bacterial growth suggest functional redundancy in carbon mineralization. Appl Environ Microbiol 75:1589–1596. CrossRefPubMedPubMedCentralGoogle Scholar
  45. Smith P (2016) Soil carbon sequestration and biochar as negative emission technologies. Glob Chang Biol 22:1315–1324. CrossRefPubMedGoogle Scholar
  46. Sohi S, Lopez-Capel E, Krull E, Bol R (2009) Biochar, climate change and soil: a review to guide future research. CSIRO land and water science report series ISSN: 1834-6618: 56 pp.Google Scholar
  47. Staddon PL, Ramsey CB, Ostle N, Ineson P, Fitter AH (2003) Rapid turnover of hyphae of mycorrhizal Fungi determined by AMS microanalysis of 14C. Science 300:1138–1140. CrossRefPubMedGoogle Scholar
  48. Steinbeiss S, Gleixner G, Antonietti M (2009) Effect of biochar amendment on soil carbon balance and soil microbial activity. Soil Biol Biochem 41:1301–1310CrossRefGoogle Scholar
  49. Wang J, Xiong Z, Kuzyakov Y (2016) Biochar stability in soil: meta-analysis of decomposition and priming effects. GCB Bioenergy 8:512–523. CrossRefGoogle Scholar
  50. Wardle DA, Nilsson MC, Zackrisson O (2008) Fire-derived charcoal causes loss of forest humus. Science 320:629CrossRefPubMedGoogle Scholar
  51. Warnock DD, Lehmann J, Kuyper TW, Rillig MC (2007) Mycorrhizal responses to biochar in soil – concepts and mechanisms. Plant Soil 200:9–20CrossRefGoogle Scholar
  52. Yeates GW, Bongers T, Degoede RGM, Freckman DW, Georgieva SS (1993) Feeding habits in soil nematode families and genera – an outline for soil ecologists. J Nematol 25:315–331PubMedPubMedCentralGoogle Scholar
  53. Zheng H, Wang X, Luo X, Wang Z, Xing B (2018) Biochar-induced negative carbon mineralization priming effects in a coastal wetland soil: roles of soil aggregation and microbial modulation. Sci Total Environ 610-611:951–960. CrossRefPubMedGoogle Scholar
  54. Zhu YG, Miller RM (2003) Carbon cycling by arbuscular mycorrhizal fungi in soil-plant systems. Trends Plant Sci 8:407–409CrossRefPubMedGoogle Scholar

Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  1. 1.Centre for Ecology and HydrologyPenicuikUK
  2. 2.Lancaster Environment CentreLancaster UniversityLancasterUK
  3. 3.School of Earth and Environmental Sciences, Michael Smith BuildingThe University of ManchesterManchesterUK
  4. 4.The James Hutton InstituteDundeeUK
  5. 5.SRUC – Scotland’s Rural CollegeEdinburghUK
  6. 6.Centralised Chemistry FacilityCentre for Ecology and HydrologyBailriggUK
  7. 7.Agroécologie, AgroSup Dijon, INRAUniv. Bourgogne Franche-ComtéDijonFrance

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