Grazing and enclosure alter the vertical distribution of organic nitrogen pools and bacterial communities in semiarid grassland soils
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Background and aims
Different grazing management practices have a significant impact on the sustainability of grassland ecosystems. This study invested the vertical distribution of soil nitrogen (N) forms and soil microbial community structures in a semiarid grassland ecosystem under different grazing management practices in Inner Mongolia.
Soil samples were collected from three semiarid grassland plots subjected to different long-term management practices namely, free grazing (FG) and two different periods of enclosure (E83, enclosed since 1983 and E97, enclosed since 1997). The soil organic nitrogen (N) pools were analyzed by classical methods, and the bacterial community was assessed by PCR-DGGE and high-throughput sequencing.
The surface soil N-supplying capacity was in the order of E97 ≥ E83 ≥ FG. The soil ammonium N, amino N, and N-supplying capacity were greater in the enclosed plots than in the FG plot. Additionally, the 0–40-cm soil layer showed the influence of different management practices on the soil properties. The structure and diversity of the soil microbial community also varied with the management type. The soil organic N composition was significantly related to the soil bacterial community structure and microbial categories.
An appropriate number of years of fencing helps to improve the soil surface nutrient status, whereas overgrazing and prolonged enclosure are not conducive to the restoration of soil nutrients. Different grazing management practices can affect the microbial community structure and turnover of soil N in grasslands.
KeywordsGrazing Enclosure Soil microbial community structure Soil organic nitrogen Nitrogen-supplying capacity
This work was supported by the National Key Basic Research Program of China (2015CB150502), the National Natural Science Foundation of China (41401266), the Science & Research Program of Zhejiang Province (2016C32084), and The National Key Research and Development Program of China (2016YFD0801103).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
- Bao SD (2000) Soil and agricultural chemistry analysis. Nanjing, ChinaGoogle Scholar
- Bremner JM (1965) Total nitrogen. In: Black CA (ed) Methods of soil analysis, part 2. Soil Science Society American, Madison, pp 1149–1178Google Scholar
- Jin FH (2007) A comparative study of soil N-supplying capacity measurement method. Northwest Agriculture and Forestry UniversityGoogle Scholar
- Kang L, Han X, Zhang Z, Sun OJ (2007) Grassland ecosystems in China: review of current knowledge and research advancement. Philosophical Transactions of the Royal Society B: Biological Sciences 362(1482):997-1008Google Scholar
- Li X, Zhang S (2002) Nitrogen forms and effects of grazing in dark chestnut soil of Inner Mongolia grassland. Acta Pratacul Sin 2:15–21Google Scholar
- Li J, Zhang Q, Li Y, Liu J, Pan H, Guan X, Xu X, Xu J, Di H (2016) Impact of mowing management on nitrogen mineralization rate and fungal and bacterial communities in a semiarid grassland ecosystem. J Soils Sediments:1–12Google Scholar
- Liu JW, Zhang YJ, Li YJ, Deli W (2008) Overview of grassland and its development in China. World Grassland and Rangeland Congress 2008:3–10Google Scholar
- Oksanen J, Blanchet F, Kindt R, Legendre P, Minchin P, O’Hara, et al (2012) Package ‘vegan’. Community Ecology Package, 2, 129Google Scholar
- Parson JW, Tinsley J (1975) Nitrogenous substances. In Gieseking, J. E. (ed.) Soil Components. Volume 1. Springer Verlag, New York. pp. 263–304Google Scholar
- Qiao J (2013) Negative feedback regulation of Inner Mongolia typical grassland ecosystem nitrogenGoogle Scholar
- Ren HY, Han GD, Ohm M, Schönbach P, Gierus M, Taube F. (2015) Do sheep grazing patterns affect ecosystem functioning in steppe grassland ecosystems in Inner Mongolia?. Agriculture, Ecosystems & Environment 213:1-10Google Scholar
- Roberts TL, Norman RJ, Slayton NA, Wilson CE (2016). U.S. Patent No. 9,354,235. Washington, DC: U.S. Patent and Trademark OfficeGoogle Scholar
- Smith KA, Li S (1993) Estimation of potentially mineralisable nitrogen in soil by KCl extraction. Plant and Soil 157(2):167–174Google Scholar
- Stevenson FJ (1982) Organic forms of soil nitrogen (no. nitrogeninagrics, pp. 67-122). American Society of Agronomy, Crop Science Society of America, Soil Science Society of AmericaGoogle Scholar
- Tan H, Yan R, Yan Y, Chen B, Xin X (2015) PLFA analysis of soil microbial community structure in warm meadow steppe under different grazing intensities. Acta Pratacultural Sci 3Google Scholar
- Zaman M, Di HJ, Cameron KC, Cameron KC, Frampton CM (1999) Gross nitrogen mineralization and nitrification rates and their relationships to enzyme activities and the soil microbial biomass in soils treated with dairy shed effluent and ammonium fertilizer at different water potentials. Biol Fertil Soils 29:178–186CrossRefGoogle Scholar
- Zhang Yongsheng (2010) The influence of grazing on the physicochemical properties and bacterial diversity of the baikal impermeable soil. (Doctoral dissertation, Chinese academy of agricultural sciences)Google Scholar
- Zhao Shuai (2011) Overgrazing and enclosed original of Hulun Buir steppe soil microbial diversity. Chinese Academy of Agricultural SciencesGoogle Scholar