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Induction of S-nitrosoglutathione reductase reduces root cadmium uptake by inhibiting Iron-regulated transporter 1

  • Mei Yan Guan
  • Ya Xin Zhu
  • Xing Xing Liu
  • Chong Wei JinEmail author
Regular Article
  • 81 Downloads

Abstract

Background and aims

Increasing cadmium (Cd) contamination of agricultural soils is a serious problem. Identification of the mechanisms that control Cd uptake by roots is essential if we wish to improve the efficiency of plants in removing Cd from contaminated soils.

Methods

We dissected the role and the mechanism of S-nitrosoglutathione reductase (GSNOR) in regulating root Cd uptake in Arabidopsis plants using GSNOR-related mutants and pharmacological methods.

Results

Exposure to Cd stimulated the activity of GSNOR in roots. Both treatment with S-nitrosoglutathione (substrate of GSNOR) in wild-type plants and loss of GSNOR function in gsnor mutants improved expression of IRON-REGULATED TRANSPORTER 1 (IRT1) and increased root Cd uptake, thereby elevating the Cd levels in plants. The opposite patterns were observed in the GSNOR over-expression transgenic plant GSNOROE, suggesting a negative regulation of IRT1 expression and Cd uptake by GSNOR. However, both the improvement of Cd uptake owing to S-nitrosoglutathione treatment or GSNOR mutation and the inhibition of Cd uptake due to GSNOR over-expression, could be blocked by loss of function of IRT1 in plants.

Conclusions

We concluded that induction of GSNOR reduced Cd uptake because of its negative regulation of IRT1 in roots, which lowered Cd accumulation in plants.

Keywords

Cadmium S-nitrosoglutathione reductase IRON-REGULATED TRANSPORTER 1 Cadmium uptake, Arabidopsis 

Notes

Acknowledgments

The authors thank Dr. Elizabeth Vierling and Dr. Takafumi Mizuno for sharing seeds. This work was financially supported by the National Natural Science Foundation of China (31622051 and 31670258).

Author contributions

MYG, XXL and CWJ conceived the research plans and designed the experiment. MYG and YXZ performed experiments and analysed data. MYG and CWJ wrote the manuscript.

Supplementary material

11104_2019_4014_MOESM1_ESM.docx (1.8 mb)
ESM 1 (DOCX 1.76 MB)

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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Mei Yan Guan
    • 1
    • 2
  • Ya Xin Zhu
    • 1
  • Xing Xing Liu
    • 1
  • Chong Wei Jin
    • 1
    Email author
  1. 1.State Key Laboratory of Plant Physiology and Biochemistry, College of Environmental and Resource SciencesZhejiang UniversityHangzhouChina
  2. 2.China National Rice Research InstituteChinese Academy of Agricultural SciencesHangzhouChina

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