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Factors influencing the release of the biological nitrification inhibitor 1,9-decanediol from rice (Oryza sativa L.) roots

  • Xiaonan Zhang
  • Yufang Lu
  • Ting Yang
  • Herbert J. Kronzucker
  • Weiming ShiEmail author
Regular Article
  • 130 Downloads

Abstract

Aims

Root exudates of rice (Oryza sativa L.) can inhibit nitrification in Nitrosomonas bioassays, and 1,9-decanediol was recently identified as an important new biological nitrification inhibitor (BNI) from rice. However, the release characteristics of 1,9-decanediol have not been studied. The present study was designed to identify the major factors influencing the release of 1,9-decanediol from rice roots.

Methods

Rice plants were hydroponically grown in controlled environment chambers for 6 weeks, and root exudates were collected. Responses of exudate release to nitrogen form and concentration, pH, aeration, and bacterial inoculation were explored. The pH of root exudates, collected under different nitrogen-provision regimes, was determined, and 1,9-decanediol levels in exudates were monitored.

Results

Ammonium (NH4+) and low pH in the root environment stimulated the release of 1,9-decanediol from rice roots. When only a part of the root system was exposed to NH4+, the secretion of 1,9-decanediol was triggered in the whole root system. Aeration of the root environment significantly enhanced 1,9-decanediol release. The presence of two major nitrifiers (Nitrosomonas europaea and Nitrosomonas stercoris) in the root medium stimulated release of 1,9-decanediol, whereas denitrifiers had no effect.

Conclusions

Our results demonstrate that the release of 1,9-decanediol is enhanced by low to moderate concentrations of NH4+ (≤1.0 mM), low pH, and aeration of the rhizosphere. Our study provides the first evidence of significant 1,9-decanediol secretion induced by nitrifying bacteria.

Keywords

Biological nitrification inhibition/inhibitor (BNI) 1,9-decanediol Rice (Oryza sativa L.) Ammonium Nitrifiers 

Notes

Acknowledgements

This work was funded by grants from the National Natural Science Foundation of China (31761143015 and 31501836), the Strategic Priority Research Program (B) – ‘Soil–microbial system function and regulation’ of the Chinese Academy of Sciences (XDB15030100), the Natural Science Foundation of Jiangsu Province (BK20151053), the Leading Project of the Institute of Soil Science, Chinese Academy of Sciences (ISSASIP1606), and grants from the University of Melbourne, Australia.

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Authors and Affiliations

  1. 1.State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil ScienceChinese Academy of SciencesNanjingChina
  2. 2.University of Chinese Academy of SciencesBeijingChina
  3. 3.School of Agriculture and Food, Faculty of Veterinary and Agricultural SciencesThe University of MelbourneMelbourneAustralia

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