Plant and Soil

, Volume 434, Issue 1–2, pp 425–440 | Cite as

Leaf endophytes mediate fertilizer effects on plant yield and traits in northern oat grass (Trisetum spicatum)

  • Heather Buckley
  • Carolyn A. Young
  • Nikki D. Charlton
  • Will Q. Hendricks
  • Beth Haley
  • Padmaja Nagabhyru
  • Jennifer A. RudgersEmail author
Regular Article



Symbiotic fungi commonly increase plant acquisition of soil nutrients. Because prior work has focused on root fungi, we examined how leaf endophytes (Epichloë) influenced plant responses to fertilization and altered plant traits that may cascade to food webs and ecosystem processes.


We manipulated endophyte presence/absence in two populations of Trisetum spicatum, a wild relative of oat, under a 2 × 2 addition of soil nitrogen (N) and phosphorus (P) in the greenhouse.


Endophyte symbiosis altered how plant biomass responded to soil N and how plant traits responded to soil P. Endophytes boosted the biomass gains from N-fertilization in one population. Plants from a second population had weak benefits of symbiosis, but the endophyte altered plant traits, by increasing specific leaf area under P-fertilization, root diameter under low P, and concentration of the fungal alkaloid AcAP under N fertilization. Endophyte presence suppressed the typically observed increase in root hair density in response to soil P limitation. Under low P, symbiotic plants from both populations had improved forage quality relative to symbiont-free plants, although N-fertilization had a larger effect size on forage quality than did symbiosis. Finally, the two populations differed in production of fungal alkaloids, which generally increased in response to fertilization.


Predicting how microbial symbionts mediate plant acquisition of nutrients requires understanding how much their effects vary among plant and endophyte genotypes. Here, the magnitude and direction of leaf symbionts’ effects on plant yield and traits varied between populations and with soil nutrient availability.


Epichloë Mutualism Nitrogen Phosphorus Poaceae Mountain ecosystem 



Thanks to Terri Tobias and Andrea Porras-Alfaro for providing seeds from Niwot Ridge, CO. Thanks to Josh Lynn for assistance with SLA measurements. Thanks to Bonnie Watson and David Huhman (Analytical Chemistry Core Facility) at the Noble Research Institute for evaluating the chanoclavine and peramine, and Christopher Schardl at University of Kentucky for advice on analysis of foliar aminopyrrolizidines. Comments from two anonymous reviewers and Section Editor Thomas W. Kuyper improved the manuscript. This work was funded by NSF DEB#1354972 and support from the Rocky Mountain Biological Laboratory to J.R. and supported the undergraduate thesis work of H.B.

Supplementary material

11104_2018_3848_MOESM1_ESM.docx (6.2 mb)
ESM 1 (DOCX 6368 kb)


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Copyright information

© Springer Nature Switzerland AG 2018

Authors and Affiliations

  1. 1.Department of BiologyUniversity of New MexicoAlbuquerqueUSA
  2. 2.Noble Research Institute, LLCArdmoreUSA
  3. 3.Rocky Mountain Biological LaboratoryGothicUSA
  4. 4.Department of Plant PathologyUniversity of KentuckyLexingtonUSA

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