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Plant and Soil

, Volume 431, Issue 1–2, pp 89–105 | Cite as

Bacillus methylotrophicus CSY-F1 alleviates drought stress in cucumber (Cucumis sativus) grown in soil with high ferulic acid levels

  • Xinwei Hou
  • Fenghui Wu
  • Xiu-Juan Wang
  • Zhong-Tao Sun
  • Yue Zhang
  • Ming-Tao Yang
  • Hongrui Bai
  • Songwen Li
  • Ji-Gang BaiEmail author
Regular Article
  • 278 Downloads

Abstract

Aims

Drought and ferulic acid (FA) inhibit plant growth. Here, we investigated whether Bacillus methylotrophicus CSY-F1 alleviates drought stress in cucumber (Cucumis sativus) plants grown in high-FA soil.

Methods

Cucumber seedlings grown in high-FA soil were inoculated with CSY-F1 for 20 d, and then subjected to drought for 3 d.

Results

In rhizospheric soil of drought-stressed seedlings, CSY-F1 decreased FA levels and increased soil water contents, polysaccharide levels, and catalase, phosphatase, urease, and sucrase activities at low or high FA concentrations. In drought-stressed seedlings grown in FA-containing soil, CSY-F1 improved plant growth, and reduced leaf wilting; CSY-F1 decreased superoxide radical, hydrogen peroxide, and malonaldehyde levels. CSY-F1 increased superoxide dismutase (SOD), catalase, guaiacol peroxidase, ascorbate peroxidase, dehydroascorbate reductase, monodehydroascorbate reductase, and glutathione reductase activities in these seedlings. In addition, CSY-F1 elevated plant relative water content and osmotic potential, and enhanced ascorbate and glutathione contents, proline and soluble sugar levels, and catalase, copper/zinc SOD, manganese SOD, CsPYL1, and CsPYL2 transcript levels.

Conclusion

CSY-F1 increases the polysaccharide levels and enzyme activities in soil, and enhances antioxidant enzyme activities, proline and soluble sugar levels, and transcript levels of CsPYL1 and CsPYL2 in leaves, thus alleviating drought stress in cucumber under FA conditions.

Keywords

Antioxidant enzyme Bacillus methylotrophicus Cucumis sativus Drought Ferulic acid RT-PCR 

Notes

Acknowledgments

This work was supported by the Science and Technology Major Project of Shandong Province, China (No. 2015ZDXX0502B04). We are grateful to Dr. Qi Wang (Biocontrol of Plant Diseases and Microbiological Laboratory, China Agricultural University) for providing plasmid pGFP4412.

Supplementary material

11104_2018_3748_MOESM1_ESM.docx (38 kb)
ESM 1 (DOCX 38.3 kb)

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Copyright information

© Springer Nature Switzerland AG 2018

Authors and Affiliations

  • Xinwei Hou
    • 1
  • Fenghui Wu
    • 2
  • Xiu-Juan Wang
    • 1
  • Zhong-Tao Sun
    • 1
  • Yue Zhang
    • 1
  • Ming-Tao Yang
    • 1
  • Hongrui Bai
    • 3
  • Songwen Li
    • 1
  • Ji-Gang Bai
    • 1
    Email author
  1. 1.State Key Laboratory of Crop Biology, College of Life SciencesShandong Agricultural UniversityTai’anPeople’s Republic of China
  2. 2.State Key Laboratory of Crop Biology, College of Horticulture Science and EngineeringShandong Agricultural UniversityTai’anPeople’s Republic of China
  3. 3.Tai’an No. 1 High School of Shandong ProvinceTai’anPeople’s Republic of China

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