Bacillus methylotrophicus CSY-F1 alleviates drought stress in cucumber (Cucumis sativus) grown in soil with high ferulic acid levels
- 278 Downloads
Drought and ferulic acid (FA) inhibit plant growth. Here, we investigated whether Bacillus methylotrophicus CSY-F1 alleviates drought stress in cucumber (Cucumis sativus) plants grown in high-FA soil.
Cucumber seedlings grown in high-FA soil were inoculated with CSY-F1 for 20 d, and then subjected to drought for 3 d.
In rhizospheric soil of drought-stressed seedlings, CSY-F1 decreased FA levels and increased soil water contents, polysaccharide levels, and catalase, phosphatase, urease, and sucrase activities at low or high FA concentrations. In drought-stressed seedlings grown in FA-containing soil, CSY-F1 improved plant growth, and reduced leaf wilting; CSY-F1 decreased superoxide radical, hydrogen peroxide, and malonaldehyde levels. CSY-F1 increased superoxide dismutase (SOD), catalase, guaiacol peroxidase, ascorbate peroxidase, dehydroascorbate reductase, monodehydroascorbate reductase, and glutathione reductase activities in these seedlings. In addition, CSY-F1 elevated plant relative water content and osmotic potential, and enhanced ascorbate and glutathione contents, proline and soluble sugar levels, and catalase, copper/zinc SOD, manganese SOD, CsPYL1, and CsPYL2 transcript levels.
CSY-F1 increases the polysaccharide levels and enzyme activities in soil, and enhances antioxidant enzyme activities, proline and soluble sugar levels, and transcript levels of CsPYL1 and CsPYL2 in leaves, thus alleviating drought stress in cucumber under FA conditions.
KeywordsAntioxidant enzyme Bacillus methylotrophicus Cucumis sativus Drought Ferulic acid RT-PCR
This work was supported by the Science and Technology Major Project of Shandong Province, China (No. 2015ZDXX0502B04). We are grateful to Dr. Qi Wang (Biocontrol of Plant Diseases and Microbiological Laboratory, China Agricultural University) for providing plasmid pGFP4412.
- Honma M, Shimomura T (1978) Metabolism of 1-aminocyclopropane-1-carboxylic acid. Agric Biol Chem 42:1825–1831Google Scholar
- Hoque MA, Banu MNA, Okuma E, Amako K, Nakamura Y, Shimoishi Y, Murata Y (2007) Exogenous proline and glycinebetaine increase NaCl-induced ascorbate- glutathione cycle enzyme activities, and proline improves salt tolerance more than glycinebetaine in tobacco Bright Yellow-2 suspension-cultured cells. J Plant Physiol 164:1457–1468CrossRefPubMedGoogle Scholar
- Radif HM, Hassan SS (2014) Role of chitinase produced from Azospirillum brasiliense in degradation free and snail chitin in soil. World J Exp Biosci 2:41–45Google Scholar
- Roberge MR (1978) Methodology of enzymes determination and extraction. In: Burns RG (ed) Soil Enzymes. Academic Press, New York, pp 341–373Google Scholar
- Sardans J, Penuelas J (2010) Soil enzyme activity in a Mediterranean forest after six years of drought. Soil Biol Biochem 74:838–851Google Scholar
- Tian T, Qi XC, Wang Q, Mei RH (2004) Colonization study of GFP-tagged Bacillus strains on wheat surface. Acta Phytopathol Sin 34:346–351Google Scholar
- Verma P, Yadav AN, Khannam KS, Panjiar N, Kumar S, Saxena AK, Suman A (2015) Assessment of genetic diversity and plant growth promoting attributes of psychrotolerant bacteria allied with wheat (Triticum aestivum) from the northern hills zone of India. Ann Microbiol 65:1885–1899CrossRefGoogle Scholar