Copper oxide nanoparticle effects on root growth and hydraulic conductivity of two vegetable crops
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Root growth and water transport were evaluated for two vegetable crops of contrasting root architecture (lettuce, carrot) exposed to copper oxide nanoparticles (CuO NPs).
10-day seedling root growth assays were evaluated for 16 nanometer (nm) diameter CuO NP and CuCl2 control (0.8 – 798.9 mg Cu L-1). In a separate experiment, hydraulic conductivity (Kh) of root systems not previously exposed to NP was tested using 16 and 45 nm CuO NP (798.9 mg Cu L-1) relative to CuO NP-free controls, and xylem sap was assessed by TEM-EDS for presence of CuO NPs.
16 nm CuO NP produced dose-dependent increases in root diameter for lettuce (+52%) and carrot (+26%) seedlings, whereas CuCl2 did not affect (lettuce) or marginally increased (carrot) root diameter. Root Kh was similarly reduced by 16 and 45 nm CuO NPs for lettuce (-46%) but not for carrot, and no Cu was identified by TEM-EDS in xylem sap.
Adverse effects of CuO NPs on root physiology and function in the early stages of growth of two key food crops are not necessarily due to Cu2+ toxicity and can be specific to crop species. In addition to triggering root thickening, reduction of root Kh signifies that CuO NPs can compromise root water transport and thus crop performance.
KeywordsCopper oxide Nanoparticles Roots Hydraulic conductivity Lettuce Carrot
This material is based upon work that is supported by the National Institute of Food and Agriculture, U.S. Department of Agriculture, under award number Grant #2013-67017-21211. We thank Professor Wendy Silk (Department of Land, Air and Water Resources, University of California-Davis) for providing intellectual support, laboratory resources and student advising. We thank Professor Thomas Young (Department of Civil and Environmental Engineering, University of California-Davis) for providing access to and support for ICP-MS analysis. Finally, we would like to thank Fred Hayes at the Applied Materials Characterization Facility at UC Davis for his support in collecting STEM data.
- Dimkpa CO, McLean JE, Latta DE, Manangón E, Britt DW, Johnson WP, Boyanov MI, Anderson AJ (2012) CuO and ZnO nanoparticles: phytotoxicity, metal speciation, and induction of oxidative stress in sand-grown wheat. J Nanopart Res 14:1125. https://doi.org/10.1007/s11051-012-1125-9 CrossRefGoogle Scholar
- Drążkiewicz M, Skórzyńska-Polit E, Krupa Z (2004) Copper-induced oxidative stress and antioxidant defence in Arabidopsis thaliana. Biometals 17:379–387. https://doi.org/10.1023/b:biom.0000029417.18154.22 CrossRefPubMedGoogle Scholar
- Du W, Tan W, Peralta-Videa JR, Gardea-Torresdey JL, Ji R, Yin Y, Guo H (2016) Interaction of metal oxide nanoparticles with higher terrestrial plants: Physiological and biochemical aspects. Plant Physiol Biochem. https://doi.org/10.1016/j.plaphy.2016.04.024
- Eduok S, Coulon F (2017) Engineered nanoparticles in the environments: interactions with microbial systems and microbial activity. In: Cravo-Laureau C, Cagnon C, Lauga B, Duran R (eds) Microbial ecotoxicology. Springer International Publishing, ChamGoogle Scholar
- Fleischer A, O'Neill MA, Ehwald R (1999) The pore size of non-graminaceous plant cell walls is rapidly decreased by borate ester cross-linking of the pectic polysaccharide rhamnogalacturonan II. Plant Physiol 121:829–838. https://doi.org/10.1104/pp.121.3.829 CrossRefPubMedPubMedCentralGoogle Scholar
- Jarbeau JA, Ewers FW, Davis SD (1995) The mechanism of water-stress-induced embolism in two species of chaparral shrubs. Plant Cell Environ 18:189–196. https://doi.org/10.1111/j.1365-3040.1995.tb00352.x CrossRefGoogle Scholar
- Maas EV, Hoffman G (1976) Crop salt tolerance-current assessment. Proc Region Saline-Seep Contr Symp 6:245–252Google Scholar
- Parfitt RL, Wilson A (1985) Estimation of allophane and halloysite in three sequences of volcanic soils, New Zealand. Catena Suppl 7:1–8Google Scholar
- Pérez-Rodríguez P, Paradelo M, Rodríguez-Salgado I, Fernández-Calviño D, López-Periago JE (2013) Modeling the influence of raindrop size on the wash-off losses of copper-based fungicides sprayed on potato (Solanum tuberosum L.) leaves. J Environ Sci Health B 48:737–746. https://doi.org/10.1080/03601234.2013.780551 CrossRefPubMedGoogle Scholar
- Rippner DA, Green PG, Young TM, Parikh SJ (2018) Dissolved organic matter reduces CuO nanoparticle toxicity to duckweed in simulated natural systems. Environmental Pollution 234: 692–698 https://doi.org/10.1016/j.envpol.2017.12.014
- Tang YJ, Wu SG, Huang L, Head J, Chen D, Kong IC (2013) Phytotoxicity of metal oxide nanoparticles is related to both dissolved metals ions and adsorption of particles on seed surfaces. J Pet Environ Biotechnol 2012.Google Scholar
- Tracy SR, Black CR, Roberts JA, Sturrock C, Mairhofer S, Craigon J, Mooney SJ (2012) Quantifying the impact of soil compaction on root system architecture in tomato (Solanum lycopersicum) by X-ray micro-computed tomography. Ann Bot 110:511–519. https://doi.org/10.1093/aob/mcs031 CrossRefPubMedPubMedCentralGoogle Scholar
- Wada K (1978) Chapter 4 Allophane and imogolite. In: Toshio S, Susumu S (eds) Developments in sedimentology. ElsevierGoogle Scholar
- Zhao L, Ortiz C, Adeleye AS, Hu Q, Zhou H, Huang Y, Keller AA (2016) Metabolomics to detect response of lettuce (Lactuca sativa) to Cu(OH)2 nanopesticides: oxidative stress response and detoxification mechanisms. Environ Sci Technol 50:9697–9707. https://doi.org/10.1021/acs.est.6b02763 CrossRefPubMedGoogle Scholar