Transcriptome analysis of sweet Sorghum inbred lines differing in salt tolerance provides novel insights into salt exclusion by roots
- 231 Downloads
Backgrounds and aims
Sweet sorghum is an annual C4 crop with a high salt tolerance. However, little is known about the molecular mechanisms of salt exclusion in roots of sweet sorghum. In this study, the physiological parameters and transcript profiles of two inbred lines of sweet sorghum roots (salt-tolerant M-81E and salt-sensitive Roma) were analyzed in the presence of 0 or 150 mM NaCl in order to elucidate the molecular mechanisms of salt exclusion.
We found that the Na+ concentrations in both shoots and roots of M-81E were lower than that of Roma. Moreover, we identified 2085 and 3172 differentially expressed genes between control plants and those subjected to salt stress in M-81E and Roma strains, respectively. The differentially expressed genes involved in pathways related to salt exclusion such as formation of root casparian bands and suberin lamellae, membrane-bound ion translocating proteins. Many of these genes underwent greater change in M-81E compared to Roma. These results revealed that the better ability of salt exclusion in M-81E may be caused by the combination of physical barrier effect of root apoplastic barriers and the transportation of Na+ out of the xylem by HKT1;5. Moreover, some genes encoding transcription factors were also differentially expressed, which may be involved in the regulation of genes related to salt exclusion.
This RNA-seq dataset provide comprehensive insights into the transcriptomic landscape to reveal molecular mechanisms of salt exclusion in roots of sweet sorghum.
KeywordsGenes Roots Salt exclusion Sweet sorghum Transcriptomic profile
cinnamoyl CoA reductase
cinnamyl alcohol dehydrogenase
calcineurin B-like protein
CBL-interacting protein kinase
Heat shock proteins
differentially expressed genes
Reads per KB per million
false discovery rate
Clusters of Orthologous Groups
Kyoto Encyclopedia of Genes and Genomes
- qRT -PCR
quantitative real-time PCR
plasma membrane intrinsic proteins
tonoplast intrinsic proteins
We are grateful for financial support from Natural Science Research Foundation of Shandong Province (ZR2016JL028, ZR2014CZ002), Major Program of Shandong Provincial Natural Science Foundation (2017C03), the NSFC (National Natural Science Research Foundation of China, project No. 31770288), Independent innovation and achievement transformation of special major key technical plans of Shandong Province (2015ZDJS03002).
ZY wrote this manuscript; ZY, HZ and XW performed experiments; ZY and JS collected data and carried out all analyses; NS and BW conceptualized the idea and revised the manuscript.
Compliance with ethical standards
The authors declare that they have no competing interests.
- Almodares A, Hadi M (2009) Production of bioethanol from sweet sorghum: a review. Afr J Agric Res:772–780Google Scholar
- Ashburner M, Ball CA, Blake JA, Botstein D, Butler H, Cherry JM, Davis AP, Dolinski K, Dwight SS, Eppig JT, Harris MA, Hill DP, Issel-Tarver L, Kasarskis A, Lewis S, Matese JC, Richardson JE, Ringwald M, Rubin GM, Sherlock G (2000) Gene ontology: tool for the unification of biology. Nat Genet 25:25–29CrossRefPubMedPubMedCentralGoogle Scholar
- Byrt CS, Xu B, Krishnan M et al (2014) The Na+ transporter, TaHKT1;5-D, limits shoot Na+accumulation in bread whea plant journal for cell. Mol Biol 80(3):516Google Scholar
- Dai LY, Zhang LJ, Jiang SJ, Yin KD (2014) Saline and alkaline stress genotypic tolerance in sweet sorghum is linked to sodium distribution. Acta Agric Scand B. Soil Plant Sci 64(6):471–481Google Scholar
- Guo YY, Tian SS, Liu SS, Wang WQ, Sui N (2018) Energy dissipation and antioxidant enzyme system protect photosystem II of sweet sorghum under drought stress. Photosynthetica 56(3):861–872Google Scholar
- Landgraf R, Smolka U, Altmann S, Eschen-Lippold L, Senning M, Sonnewald S, Weigel B, Frolova N, Strehmel N, Hause G, Scheel D, Bottcher C, Rosahl S (2014) The ABC transporter ABCG1 is required for suberin formation in potato tuber periderm. Plant Cell 26:3403–3415CrossRefPubMedPubMedCentralGoogle Scholar
- Paterson AH, Bowers JE, Bruggmann R, Dubchak I, Grimwood J, Gundlach H, Haberer G, Hellsten U, Mitros T, Poliakov A, Schmutz J, Spannagl M, Tang H, Wang X, Wicker T, Bharti AK, Chapman J, Feltus FA, Gowik U, Grigoriev IV, Lyons E, Maher CA, Martis M, Narechania A, Otillar RP, Penning BW, Salamov AA, Wang Y, Zhang L, Carpita NC, Freeling M, Gingle AR, Hash CT, Keller B, Klein P, Kresovich S, McCann MC, Ming R, Peterson DG, Mehboob-ur-Rahman, Ware D, Westhoff P, Mayer KFX, Messing J, Rokhsar DS (2009) The Sorghum bicolor genome and the diversification of grasses. Nature 457:551–556CrossRefPubMedGoogle Scholar
- Serra O, Soler M, Hohn C, Sauveplane V, Pinot F, Franke R, Schreiber L, Prat S, Molinas M, Figueras M (2009) CYP86A33-targeted gene silencing in potato tuber alters suberin composition, distorts suberin lamellae, and impairs the periderm's water barrier function. Plant Physiol 149:1050–1060CrossRefPubMedPubMedCentralGoogle Scholar
- Steudle E, Peterson CA (1998) How does water get through roots? J Exp Bot 49:775–788Google Scholar
- Yang Z, Wang Y, Wei X, Zhao X, Wang B, Sui N (2017) Transcription profiles of genes related to hormonal regulations under salt stress in sweet. Sorghum Plant Mol Biol Report 36(6):586–599Google Scholar
- Yeo A, Flowers T (1986) Salinity resistance in Rice (Oryza sativa L.) and a pyramiding approach to breeding varieties for saline soils. Funct Plant Biol 13:161–173Google Scholar
- Zhong S et al. (2011) High-throughput illumina strand-specific RNA sequencing library preparation. Cold Spring Harb Protoc 2011(8):940–949Google Scholar