Cheatgrass-associated AMF community negatively affects sagebrush root production but not C transfer to the soil
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Cheatgrass (Bromus tectorum) invasion can alter community structure of arbuscular mycorrhizal fungi (AMF) in the sagebrush-steppe ecosystem. The feedbacks and underlying mechanisms of a changed AMF community on sagebrush (Artemisia tridentate ssp. wyomingensis) remain unclear. We assessed how ‘own’ versus ‘foreign’ AMF impact plant biomass, C transfer to AMF, and decomposition rates.
To evaluate the impact of different AMF communities on plant biomass and C transfer, sagebrush and cheatgrass were grown in sterilized soil amended with ‘own’ or ‘foreign’ AMF. Sagebrush plants were labeled with 13C-CO2 to assess changes in allocation of C belowground (13C-PLFA & NLFA) and decomposition (soil respired 13C-CO2). Community structure and alpha-diversity of AMF were examined in native and cheatgrass-invaded communities.
Cheatgrass invasion changed AMF community structure and decreased AMF taxon richness. Sagebrush C transfer and decomposition were not altered, but sagebrush root and cheatgrass shoot production was reduced with ‘foreign’ AMF and no AMF, respectively.
Our results from the greenhouse experiment suggest that sagebrush performance declines with cheatgrass invasion. This may be caused by a disadvantageous AMF community shift, where ‘foreign’ AMF received the same amount of C but provided fewer benefits to sagebrush, as shown by decreased root biomass. These findings provide insight into the feedback mechanism that may contribute to decreasing native plant performance upon invasion.
KeywordsCarbon transfer Decomposition Invasion Mycorrhizae Plant-soil feedback
arbuscular mycorrhizal fungi
- CG AMF
- SB AMF
distance-based redundancy analysis
fatty acid methyl esters
gas chromatography-combustion-isotope ratio mass spectrometer
neutral lipid fatty acids
Orchard Training Area
phospholipid fatty acids
amplicon sequence variant
Vienna-Pee Dee Belemnite
We thank Marcelo Serpe and Kevin Feris for their comments on earlier versions of this manuscript, and Ian DuVall, Mary Finnell, Jamie (Hicks) Kezar, Leslie Nichols, Arianne Shannon, Aislinn Johns, Jaron Adkins, and Shay Gillette for assisting with field and laboratory work. Thanks to Colorado State University Ecolab for training and lab space for PLFA samples.
This work was supported by the Idaho National Guard under award number 5484, and by the National Science Foundation Idaho Established Program to Stimulate Competitive Research (NSF IdahoEPSCoR) Program under award number EPS-0814387. Any use of trade, product, or firm names is for descriptive purposes only and does not imply endorsement by the US Government.
- Allen EB (1984) VA mycorrhizae and colonizing annuals: implications for growth, competition, and succession. In VA mycorrhizae and reclamation of arid and semi-arid lands. Wyoming agricultural Experiment Station scientific report SA1261. Ed. SE Williams, MF Allen. pp 42–52. University of Wyoming, LaramieGoogle Scholar
- Brennan PJ (1988) Mycobacterium and other actinomycetes. In: Ratledge C, Wilkinson SG (eds) Microbial Lipids. Academic Press, London, pp 203–298Google Scholar
- Caporaso JG, Kuczynski J, Stombaugh J, Bittinger K, Bushman FD, Costello EK, Fierer N, Peña AG, Goodrich JK, Gordon JI, Huttley GA, Kelley ST, Knights D, Koenig JE, Ley RE, Lozupone CA, Mc Donald D, Muegge BD, Pirrung M, Reeder J, Sevinsky JR, Turnbaugh PJ, Walters WA, Widmann J, Yatsunenko T, Zaneveld J, Knight R (2010) QIIME allows analysis of high-throughput community sequencing data. Nature 7:335–336Google Scholar
- Caporaso JG, Lauber CL, Walters WA, Berg-Lyons D, Huntley J, Fierer N, Owens SM, Betley J, Fraser L, Bauer M, Gormley N, Gilbert JA, Smith G, Knight R (2012) Ultra-high-throughput microbial community analysis on the Illumina HiSeq and MiSeq platforms. ISME J:1–4Google Scholar
- Chapin IIIFS, Matson PA, Mooney HA (2002) Principles of terrestrial ecosystem ecology. Springer-Verlag, New YorkGoogle Scholar
- Griffiths BS, Ritz K, Bardgett RD, Cook R, Christensen S, Ekelund F, Sørensen SJ, Bååth E, Bloem J, Ruiter PC, Dolfing J, Nicolardot B (2000) Ecosystem response of pasture soil communities to fumigation-induced microbial diversity reductions: an examination of the biodiversity-ecosystem function relationship. Oikos 90:279–294CrossRefGoogle Scholar
- Kroppenstedt RM (1985) Fatty acid and menaquinone analysis of actinomycetes and related organisms. In: Goodfellow M, Mminnikin DE (eds) Chemical methods in bacterial systematics. Academic Press, London, pp 173–199Google Scholar
- Leff JW (2016) mctoolsr: Microbial Comunity Data Analysis Tools. https://github.com/leffj/mctoolsr
- Linderman RG (1994) Role of VAM fungi in biocontrol. In: Pfleger FL, Linderman RG (eds) Mycorrhizae and plant health. APS, St Paul, pp 1–26Google Scholar
- Lindsey DL (1984) The role of vesicular-arbuscular mycorrhizae in shrub establishment. In VA mycorrhizae and reclamation of arid and semi-arid lands. Wyoming agricultural Experiment Station scientific report SA1261. Ed. SE Williams, MF Allen. pp. 53–68. University of Wyoming, LaramieGoogle Scholar
- Miller RF, Knick ST, Pyke DA, Meinke CW, Hanser SE, Wisdom MJ, Hild AL (2011) Characteristics of sagebrush habitats and limitations to long-term conservation. In: Knick ST, Connelly JW (eds) Greater sage-grouse: ecology and conservation of a landscape species and its habitats. Studies in avian biology 38. University of California Press, Berkeley, CA, USA, pp 145–184Google Scholar
- Miller RF, Chambers JC, Pyke DA, Pierson FB, Williams CJ (2013) A review of fire effects on vegetation and soils in the great basin region: response and ecological site characteristics. Gen. Tech. Rep. RMRS-GTR-308. Fort Collins, CO: U.S. Department of Agriculture, Forest Service, Rocky Mountain Research Station. 126 pGoogle Scholar
- Olsson PA, Bååth E, Jakobsen I, Söderström B (1995) The use of phospholipid and neutral lipid fatty acids to estimate biomass of arbuscular mycorrhizal fungi in soil. Mycol Res 99:623–629Google Scholar
- Olsson PA, Bååth E, Jakobsen I (1997) Phosphorus effects on the mycelium and storage structures of an arbuscular mycorrhizal fungus as studied in the soil and roots by analysis of fatty acid signatures. Appl Environ Microbiol 63:3531–3538Google Scholar
- Olsson PA (1999) Signature fatty acids provide tools for determination of the distribution and interactions of mycorrhizal fungi in soil. FEMS Microbiol Ecol 29:303–310Google Scholar
- Olsson PA, Johansen A (2000) Lipid and fatty acid composition of hyphae and spores of arbuscular mycorrhizal fungi at different growth stages. Mycol Res 104:429–434Google Scholar
- Olsson PA, Wilhelmsson P (2000) The growth of external AM fungal mycelium in sand dunes and in experimental systems. Plant Soil 226:161–169Google Scholar
- Olsson PA, Johnson NC (2005) Tracking carbon from the atmosphere to the rhizosphere. Ecol Let 8:1264–1270Google Scholar
- Smith SE, Read DJ (2008) Mycorrhizal symbiosis. Academic Press, CambridgeGoogle Scholar
- U.S. Department of Agriculture Natural Resources Conservation Service (2015) Available via https://www.nrcs.usda.gov/wps/portal/nrcs/main/national/technical/nra/nri/
- U.S. Fish and Wildlife Service (2016) Greater sage-grouse website, created by the Mountain-Prairie Region of the U.S. Fish and Wildlife Service. Last modified: June 17, Available via https://www.fws.gov/greatersagegrouse/sagesteppe.php
- Walder F, Niemann H, Natarajan M, Lehmann MF, Boller T, Wiemken A (2012) Mycorrhizal Networks: Common goods of plants shared under unequal terms of trade. Plant Physiol 159:789–797Google Scholar
- Walder F, van der Heijden MGA (2015) Regulation of resource exchange in the arbuscular mycorrhizal symbiosis. Nature Plants 1:1–7Google Scholar