Consequences of mixing Acacia mangium and Eucalyptus grandis trees on soil exploration by fine-roots down to a depth of 17 m
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Background and aims
Fine-root functioning is a major driver of plant growth and strongly influences the global carbon cycle. While fine-root over-yielding has been shown in the upper soil layers of mixed-species forests relative to monospecific stands, the consequences of tree diversity on fine-root growth in very deep soil layers is still unknown. Our study aimed to assess the consequences of mixing Acacia mangium and Eucalyptus grandis trees on soil exploration by roots down to the water table at 17 m depth in a tropical planted forest.
Fine roots (diameter < 2 mm) were sampled in a randomized block design with three treatments: monospecific stands of Acacia mangium (100A), Eucalyptus grandis (100E), and mixed stands with 50% of each species (50A50E). Root ingrowth bags were installed at 4 depths (from 0.1 m to 6 m) in the three treatments within three different blocks, to study the fine-root production over 2 periods of 3 months.
Down to 17 m depth, total fine-root biomass was 1127 g m−2 in 50A50E, 780 g m−2 in 100A and 714 g m−2 in 100E. Specific root length and specific root area were 110–150% higher in 50A50E than in 100A for Acacia mangium trees and 34% higher in 50A50E than in 100E for Eucalyptus grandis trees. Ingrowth bags showed that the capacity of fine roots to explore soil patches did not decrease down to a depth of 6 m for the two species.
Belowground interactions between Acacia mangium and Eucalyptus grandis trees greatly increased the exploration of very deep soil layers by fine roots, which is likely to enhance the uptake of soil resources. Mixing tree species might therefore increase the resilience of tropical planted forests through a better exploration of deep soils.
KeywordsPlantation Forest Deep root Fine-root density Root traits Diversity Over-yielding Brazil
We are truly grateful to Daise Silva Ferreira for the huge work carried out in the field and in the laboratory. We would like to thank Agence national de la recherche (Intens&fix Project ANR-2010-STRA-004-03) and São Paulo Research foundation (FAFESP, projects 2015/24911-8 and 2011/20510-8) for their financial support. The study belongs to the SOERE F-ORE-T, which is supported annually by Ecofor, Allenvi and the French National Research Infrastructure ANAEE-F (http://www.anaee-france.fr/fr/). We are grateful of the technical support of Rildo M. Moreira and the staff of Itatinga Research Station (ESALQ/USP) as well as Eder Araujo da Silva (http://www.floragroapoio.com.br).
- Battie-Laclau P, Laclau JP, Domec JC, Christina M, Bouillet JP, Cassia Piccolo M, Moraes Gonçalves JL, Krusche AV, Bouvet JM, Nouvellon Y (2014) Effects of potassium and sodium supply on drought-adaptive mechanisms in Eucalyptus grandis plantations. New Phytol 203:401–413CrossRefPubMedGoogle Scholar
- Bauhus J, Messier C (1999) Soil exploitation strategies of fine roots in different tree species of the southern boreal forest of eastern Canada. Can J For Res 29:260–273Google Scholar
- Bini D, Dos Santos CA, Bouillet J-P, de Morais Goncalves JL, Cardoso EJBN (2013) Eucalyptus grandis and Acacia mangium in monoculture and intercropped plantations: evolution of soil and litter microbial and chemical attributes during early stages of plant development. Appl Soil Ecol 63:57–66CrossRefGoogle Scholar
- Bouillet J-P, Laclau J-P, Gonçalves JLM, Moreira M, Trivelin P, Jourdan C, Silva E, Piccolo MC, Tsai S, Galiana A (2008) Mixed-species plantations of Acacia Mangium and Eucalyptus Grandis in Brazil: 2: nitrogen accumulation in the stands and biological N2 fixation. For Ecol Manag 255:3918–3930CrossRefGoogle Scholar
- Bouillet J-P, Laclau J-P, Gonçalves JLM, Voigtlaender M, Gava JL, Leite FP, Hakamada R, Mareschal L, Mabiala A, Tardy F, Levillain J, Deleporte P, Epron D, Nouvellon Y (2013) Eucalyptus and acacia tree growth over entire rotation in single- and mixed-species plantations across five sites in Brazil and Congo. For Ecol Manag 301:89–101. https://doi.org/10.1016/j.foreco.2012.09.019 CrossRefGoogle Scholar
- da Silva EV, de Moraes Gonçalves JL, de Freitas Coelho SR, e Moreira RM, de Miranda Mello SL, Bouillet J-P, Jourdan C, Laclau J-P (2009) Dynamics of fine root distribution after establishment of monospecific and mixed-species plantations of Eucalyptus grandis and Acacia mangium. Plant Soil 325:305–318CrossRefGoogle Scholar
- Derrien D, Plain C, Courty P-E, Gelhaye L, Moerdijk-Poortvliet TCW, Thomas F, Versini A, Zeller B, Koutika L-S, Boschker HTS, Epron D (2014) Does the addition of labile substrate destabilise old soil organic matter? Soil Biol Biochem 76:149–160. https://doi.org/10.1016/j.soilbio.2014.04.030 CrossRefGoogle Scholar
- Epron D, Nouvellon Y, Mareschal L, Moreira RM, Koutika L-S, Geneste B, Delgado-Rojas JS, Laclau J-P, Sola G, Gonçalves JLM, Bouillet J-P (2013) Partitioning of net primary production in eucalyptus and acacia stands and in mixed-species plantations: two case-studies in contrasting tropical environments. For Ecol Manag 301:102–111. https://doi.org/10.1016/j.foreco.2012.10.034 CrossRefGoogle Scholar
- Freschet GT, Valverde-Barrantes OJ, Tucker CM, Craine JM, McCormack ML, Violle C, Fort F, Blackwood CB, Urban-Mead KR, Iversen CM, Bonis A, Comas LH, Cornelissen JHC, Dong M, Guo D, Hobbie SE, Holdaway RJ, Kembel SW, Makita N, Onipchenko VG, Picon-Cochard C, Reich PB, Riva EG, Smith SW, Soudzilovskaia NA, Tjoelker MG, Wardle DA, Roumet C (2017) Climate, soil and plant functional types as drivers of global fine-root trait variation. J Ecol. https://doi.org/10.1111/1365-2745.12769
- Gonçalves JLM, Alvares CA, Higa AR, Silva LD, Alfenas AC, Stahl J, de Barros Ferraz SF, de Paula Lima W, PHS B, Hubner A (2013) Integrating genetic and silvicultural strategies to minimize abiotic and biotic constraints in Brazilian eucalypt plantations. For Ecol Manag 301:6–27CrossRefGoogle Scholar
- IBA (2016) Brazilian tree industry. http://iba.org/images/shared/Biblioteca/IBA_RelatorioAnual2016_.pdf. Accessed 29 jan 2017
- Kuznetsova A, Brockhoff PB, Christensen RHB (2015) lmerTest: tests in linear mixed effects models. R package version 2.0-33. https://CRAN.Rproject.org/package=lmerTest. Accessed 10 Jul 2017
- Laclau J-P, Bouillet J-P, Gonçalves J, Silva E, Jourdan C, Cunha M, Moreira M, Saint-André L, Maquère V, Nouvellon Y (2008) Mixed-species plantations of Acacia mangium and Eucalyptus grandis in Brazil: 1. Growth dynamics and aboveground net primary production. For Ecol Manag 255:3905–3917CrossRefGoogle Scholar
- Laclau J-P, Ranger J, de Moraes Goncalves JL, Maquere V, Krusche AV, M’Bou AT, Nouvellon Y, Saint-Andre L, Bouillet J-P, de Cassia PM (2010) Biogeochemical cycles of nutrients in tropical eucalyptus plantations: main features shown by intensive monitoring in Congo and Brazil. For Ecol Manag 259:1771–1785CrossRefGoogle Scholar
- Laclau JP, da Silva EA, Rodrigues Lambais G, Bernoux M, le Maire G, Stape JL, Bouillet JP, Goncalves JL, Jourdan C, Nouvellon Y (2013a) Dynamics of soil exploration by fine roots down to a depth of 10 m throughout the entire rotation in Eucalyptus grandis plantations. Front Plant Sci 4:243. https://doi.org/10.3389/fpls.2013.00243 CrossRefPubMedPubMedCentralGoogle Scholar
- Laclau JP, Nouvellon Y, Reine C, Goncalves JL, Krushe AV, Jourdan C, le Maire G, Bouillet JP (2013b) Mixing eucalyptus and acacia trees leads to fine root over-yielding and vertical segregation between species. Oecologia 172:903–913. https://doi.org/10.1007/s00442-012-2526-2 CrossRefPubMedGoogle Scholar
- Lehmann J (2003) Subsoil root activity in tree-based cropping systems. Plant Soil 255:319–331Google Scholar
- Maeght JL, Gonkhamdee S, Clement C, Isarangkool Na Ayutthaya S, Stokes A, Pierret A (2015) Seasonal Patterns of Fine Root Production and Turnover in a Mature Rubber Tree (Hevea brasiliensis Mull. Arg.) Stand-Differentiation with Soil Depth and Implications for Soil Carbon Stocks. Front Plant Sci 6:1022. https://doi.org/10.3389/fpls.2015.01022 CrossRefPubMedPubMedCentralGoogle Scholar
- Maquere V (2008) Dynamics of mineral elements under a fast-growing eucalyptus plantation in Brazil. Implications for soil sustainability. Ph.D. Thesis, Agroparitech, ParisGoogle Scholar
- Marsden C, Nouvellon Y, M’Bou AT, Saint-Andre L, Jourdan C, Kinana A, Epron D (2008) Two independent estimations of stand-level root respiration on clonal eucalyptus stands in Congo: up scaling of direct measurements on roots versus the trenched-plot technique. New Phytol 177:676–687CrossRefPubMedGoogle Scholar
- Maurice J, Laclau J-P, Re DS, de Moraes Gonçalves JL, Nouvellon Y, Bouillet J-P, Stape JL, Ranger J, Behling M, Chopart J-L (2010) Fine root isotropy in Eucalyptus grandis plantations. Towards the prediction of root length densities from root counts on trench walls. Plant Soil 334:261–275CrossRefGoogle Scholar
- McCormack ML, Dickie IA, Eissenstat DM, Fahey TJ, Fernandez CW, Guo D, Helmisaari HS, Hobbie EA, Iversen CM, Jackson RB, Leppalammi-Kujansuu J, Norby RJ, Phillips RP, Pregitzer KS, Pritchard SG, Rewald B, Zadworny M (2015) Redefining fine roots improves understanding of below-ground contributions to terrestrial biosphere processes. New Phytol 207:505–518. https://doi.org/10.1111/nph.13363 CrossRefPubMedGoogle Scholar
- Nouvellon Y, Laclau JP, Epron D, Le Maire G, Bonnefond JM, Goncalves JL, Bouillet JP (2012) Production and carbon allocation in monocultures and mixed-species plantations of Eucalyptus grandis and Acacia mangium in Brazil. Tree Physiol 32:680–695. https://doi.org/10.1093/treephys/tps041 CrossRefPubMedGoogle Scholar
- Paula RR, Bouillet J-P, Ocheuze Trivelin PC, Zeller B, Leonardo de Moraes Gonçalves J, Nouvellon Y, Bouvet J-M, Plassard C, Laclau J-P (2015) Evidence of short-term belowground transfer of nitrogen from Acacia mangium to Eucalyptus grandis trees in a tropical planted forest. Soil Biol Biochem 91:99–108. https://doi.org/10.1016/j.soilbio.2015.08.017 CrossRefGoogle Scholar
- Pierret A, Maeght JL, Clement C, Montoroi JP, Hartmann C, Gonkhamdee S (2016) Understanding deep roots and their functions in ecosystems: an advocacy for more unconventional research. Ann Bot. https://doi.org/10.1093/aob/mcw130
- Tchichelle SV, Epron D, Mialoundama F, Koutika LS, Harmand J-M, Bouillet J-P, Mareschal L (2017) Differences in nitrogen cycling and soil mineralisation between a eucalypt plantation and a mixed eucalypt and Acacia mangium plantation on a sandy tropical soil. South For J For Sci 79:1–8Google Scholar
- Team R (2013) R development core team. RA Lang Environ Stat Comput 55:275–286Google Scholar
- Voigtlaender M, Laclau J-P, de Moraes Gonçalves JL, de Cássia PM, Moreira MZ, Nouvellon Y, Ranger J, Bouillet J-P (2012) Introducing Acacia mangium trees in Eucalyptus grandis plantations: consequences for soil organic matter stocks and nitrogen mineralization. Plant Soil 352:99–111CrossRefGoogle Scholar