Plant and Soil

, Volume 407, Issue 1–2, pp 187–202 | Cite as

Expression on roots and contribution to maize phytostimulation of 1-aminocyclopropane-1-decarboxylate deaminase gene acdS in Pseudomonas fluorescens F113

  • Jordan Vacheron
  • Emeline Combes-Meynet
  • Vincent Walker
  • Brigitte Gouesnard
  • Daniel Muller
  • Yvan Moënne-Loccoz
  • Claire Prigent-CombaretEmail author
Regular Article



The plant-beneficial bacterium Pseudomonas fluorescens F113 harbours an acdS gene, which enables deamination of 1-aminocyclopropane-1-carboxylate. The impact of abiotic and biotic factors on the expression of this gene was assessed, as well as the plant-beneficial properties of F113 under different soil moistures.


An acdS-egfp biosensor was constructed in F113, validated in vitro and used to analyse, by microscopy, its expression on roots of Zea mays comparatively to Beta vulgaris. An acdS mutant was constructed and compared with the wild-type to characterize plant-beneficial effects of F113 on maize lines EP1 and FV2, under well-watered and water deficit conditions.


Different patterns of root colonization and acdS expression were observed according to plant genotype. acdS rhizoplane expression was higher on Beta vulgaris, and on maize line FV2 and hybrid PR37Y15 than on maize line EP1 and teosinte. Strain F113 but not its acdS mutant promoted root growth of EP1 under well-watered conditions and germination of FV2 under water deficit conditions.


Maize lines differed in their ability to induce acdS expression and to respond to P. fluorescens F113. The maize line leading to higher acdS expression, FV2, was the one benefiting from inoculation under water deficit.


1-aminocyclopropane-1-decarboxylate deaminase acdS expression Maize genotypes Plant growth promotion Water deficit 



E. Combes-Meynet and J. Vacheron were supported by Ph.D. grants from the Ministère de lEnseignement Supérieur et de la Recherche (Paris) and Academic Research Cluster 3 of Rhône-Alpes Region, respectively. We also acknowledge funding from the ANR project SymbioMaize (ANR-12-JSV7-0014-01). This work made use of the technical platforms Serre and Centre Technologique des Microstructures at FR BioEnviS (Université Lyon 1).

Supplementary material

11104_2016_2907_MOESM1_ESM.pdf (224 kb)
ESM 1 (PDF 224 kb)


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Copyright information

© Springer International Publishing Switzerland 2016

Authors and Affiliations

  • Jordan Vacheron
    • 1
    • 2
    • 3
    • 4
  • Emeline Combes-Meynet
    • 1
    • 2
    • 3
    • 4
  • Vincent Walker
    • 1
    • 2
    • 3
    • 4
  • Brigitte Gouesnard
    • 5
  • Daniel Muller
    • 1
    • 2
    • 3
    • 4
  • Yvan Moënne-Loccoz
    • 1
    • 2
    • 3
    • 4
  • Claire Prigent-Combaret
    • 1
    • 2
    • 3
    • 4
    Email author
  1. 1.Université de LyonLyonFrance
  2. 2.Université Lyon 1VilleurbanneFrance
  3. 3.CNRS, UMR5557, Ecologie MicrobienneUniversité Lyon 1Villeurbanne cedexFrance
  4. 4.INRA, UMR1418VilleurbanneFrance
  5. 5.INRA, UMR1334, AGAPMontpellierFrance

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