Plant and Soil

, Volume 407, Issue 1–2, pp 109–117 | Cite as

Large-scale profiling of brown rice ionome in an ethyl methanesulphonate-mutagenized hitomebore population and identification of high- and low-cadmium lines

  • Nobuhiro Tanaka
  • Sho Nishida
  • Takehiro Kamiya
  • Toru FujiwaraEmail author
Regular Article



Improving the nutritional value of cereal grains and reducing the intake of toxic elements, such as cadmium (Cd), from grains contribute to food quality.


Here, we report a large-scale profiling of the brown rice ionome in an ethyl methanesulphonate (EMS)-mutagenized population of the commercial rice cultivar ‘Hitomebore’. Broad-sense heritability for manganese, zinc, and cesium were greater than 0.5, suggesting that the EMS population is suitable for screening mutants with different concentrations of these elements.


From the EMS-mutagenized population, we isolated two mutant lines with high and low Cd levels. Sequence analysis revealed that the high-Cd line had a mutation at position 105 (leucine to histidine) in OsHMA3, a gene that regulates Cd accumulation in rice shoots. The low-Cd line had a mutation at position 242 (histidine to glutamine) in OsNramp5, a gene responsible for Cd uptake. Agronomical traits of the low-Cd line were indistinguishable from those of the wild type, indicating that the low-Cd line which we isolated is applicable to Hitomebore farming areas.


The present study shows the effectiveness of mutant lines derived from elite cultivars in developing agriculturally useful lines with different mineral contents.


Hitomebore Ionome Mineral concentration in rice grain OsNramp5 



ethyl methanesulfonate


inductively coupled plasma mass spectrometry


broad sense heritability


coefficient of variance



We greatly appreciate Dr Ryohei Terauchi for providing us with the mutagenized population of Hitomebore. Dr. Satoru Ishikawa for his constructive discussion on the present study. This study was supported in part by a grant from Steel Foundation for Environmental Protection Technology (to S. N.), and by grants from the JSPS (Grant-in-Aid for Scientific Research numbers 25221202 and 25114506) to T.F.

Supplementary material

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Fig. S1 (PPT 175 kb)
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Fig. S2 (PPT 609 kb)
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Fig. S3 (PPT 399 kb)
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Table S1 (DOCX 18 kb)


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Copyright information

© Springer International Publishing Switzerland 2016

Authors and Affiliations

  • Nobuhiro Tanaka
    • 1
  • Sho Nishida
    • 1
    • 2
  • Takehiro Kamiya
    • 1
  • Toru Fujiwara
    • 1
    Email author
  1. 1.Graduate School of Agricultural and Life SciencesThe University of TokyoTokyoJapan
  2. 2.Graduate School of Biosphere ScienceHiroshima UniversityHiroshimaJapan

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