Proteomic analysis of Allium cepa var. agrogarum L. roots under copper stress
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In the present study, the effects of Cu (2.0 and 8.0 μM) on root growth of Allium cepa var. agrogarum L. were addressed and protein abundance levels were analyzed using the technology of proteomics combined with transcriptomics, in order to go deeper into the understanding of the mechanism of Cu toxicity on plant root systems at the protein level and to provide valuable information for monitoring and forecasting the effects of exposure to Cu in real scenarios conditions.
Protein extraction; Two-dimensional electrophoresis (2-DE) analysis; Mass spectrometry analysis; Establishment of the in-house database; Restriction enzyme map of the in-house database and protein identification.
Root growth was dramatically inhibited after 12 h Cu treatment. By establishing an in-house database and using mass spectrometry analysis, 27 differentially abundant proteins were identified. These 27 proteins were involved in multiple biological processes including defensive response, transcription regulation and protein synthesis, cell wall synthesis, cell cycle and DNA replication, and other important functions.
Our results provide new insights at the proteomic level into the Cu-induced responses, defensive responses and toxic effects, and provide new molecular markers of the early events of plant responses to Cu toxicity. Moreover, the establishment of an in-house database provides a big improvement for proteomics research on non-model plants.
KeywordsCu Allium cepa var. agrogarum L Proteomics Transcriptomics Defensive responses Toxic effects
This work was supported by the National Natural Science Foundation of China (31070242), the Leading Scientists Project of Guangdong Province, the Guangdong Pearl River Scholar Funded Scheme (2012), the Research Fund for the Doctoral Program of Higher Education of China (20114407110006), the Science and Technology Program of Guangzhou, China (2014J4100053) and the Scientific Research Foundation of Graduate School of South China Normal University (2012kyjj114). The authors are grateful to Dr. Rachel Naele (QIMR Berghofer Medical Research Institute, Australia), Dr. Andy McLeod (The University of Edinburgh, Edinburgh, UK) and Prof. John Richard Schrock (Emporia State University, Kansas, USA) for language revision.
Compliance with ethical standards
Conflict of interest
We declare that we have no conflict of interest.
- Arcuri F, Papa S, Carducci A, Romagnoli R, Liberatori S, Riparbelli MG, Sanchez JC, Tosi P, del Vecchio MT (2004) Translationally controlled tumor protein (TCTP) in the human prostate and prostate cancer cells: expression, distribution, and calcium binding activity. Prostate 60:130–140CrossRefPubMedGoogle Scholar
- Gao Y, Chen XB, Zhang ZY (2007) Advances in research on lignin biosynthesis and its molecular regulation. Biotechnol Bull 2:47–51Google Scholar
- Grabherr MG, Haas BJ, Yassour M, Levin JZ, Thompson DA, Amit I, Adiconis X, Fan L, Raychowdhury R, Zeng QD, Chen ZH, Mauceli E, Hacohen N, Gnirke A, Rhind N, di Palma F, Birren BW, Nusbaum C, Lindblad-Toh K, Friedman N, Regev A (2011) Full-length transcriptome assembly from RNA-Seq data without a reference genome. Nat Biotechnol 29:644–652CrossRefPubMedPubMedCentralGoogle Scholar
- Hossain Z, Komatsu S (2013) Contribution of proteomic studies towards understanding plant heavy metal stress response. Front Plant Sci 3(article 310):1–12Google Scholar
- Kopczewski T, Kuźniak E (2013) Redox signals as a language of interorganellar communication in plant cells. Cent Eur J Biol 8:1153–1163Google Scholar
- Mota R, Pereira SB, Meazzini M, Fernandes R, Santos A, Evans CA, Philippis RD, Wright PC, Tamagnini P (2015) Effects of heavy metals on Cyanothece sp. CCY 0110 growth, extracellular polymeric substances (EPS) production, ultrastructure and protein profiles. J Proteomics 120:75–94CrossRefPubMedGoogle Scholar
- Wang YT, Björn LO (2014) Heavy metal pollution in Guangdong Province, China, and the strategies to manage the situation. Front Environ Sci 2(article 9):1–12Google Scholar