Plant and Soil

, Volume 401, Issue 1–2, pp 197–212

Proteomic analysis of Allium cepa var. agrogarum L. roots under copper stress

  • Rong Qin
  • Chanjuan Ning
  • Lars O. Björn
  • Shaoshan Li
Regular Article

DOI: 10.1007/s11104-015-2741-9

Cite this article as:
Qin, R., Ning, C., Björn, L.O. et al. Plant Soil (2016) 401: 197. doi:10.1007/s11104-015-2741-9

Abstract

Aims

In the present study, the effects of Cu (2.0 and 8.0 μM) on root growth of Allium cepa var. agrogarum L. were addressed and protein abundance levels were analyzed using the technology of proteomics combined with transcriptomics, in order to go deeper into the understanding of the mechanism of Cu toxicity on plant root systems at the protein level and to provide valuable information for monitoring and forecasting the effects of exposure to Cu in real scenarios conditions.

Methods

Protein extraction; Two-dimensional electrophoresis (2-DE) analysis; Mass spectrometry analysis; Establishment of the in-house database; Restriction enzyme map of the in-house database and protein identification.

Results

Root growth was dramatically inhibited after 12 h Cu treatment. By establishing an in-house database and using mass spectrometry analysis, 27 differentially abundant proteins were identified. These 27 proteins were involved in multiple biological processes including defensive response, transcription regulation and protein synthesis, cell wall synthesis, cell cycle and DNA replication, and other important functions.

Conclusions

Our results provide new insights at the proteomic level into the Cu-induced responses, defensive responses and toxic effects, and provide new molecular markers of the early events of plant responses to Cu toxicity. Moreover, the establishment of an in-house database provides a big improvement for proteomics research on non-model plants.

Keywords

Cu Allium cepa var. agrogarumProteomics Transcriptomics Defensive responses Toxic effects 

Supplementary material

11104_2015_2741_MOESM1_ESM.pdf (47.2 mb)
Electronic supplementary material 1(PDF 48292 kb)
11104_2015_2741_MOESM2_ESM.pdf (5.4 mb)
Electronic supplementary material 2(PDF 5493 kb)
11104_2015_2741_MOESM3_ESM.pdf (273 kb)
Electronic supplementary material 3(PDF 273 kb)
11104_2015_2741_MOESM4_ESM.pdf (144 kb)
Electronic supplementary material 4(PDF 143 kb)

Funding information

Funder NameGrant NumberFunding Note
the National Natural Science Foundation of China
  • 31070242
the Guangdong Pearl River Scholar Funded Scheme
  • 2012
the Research Fund for the Doctoral Program of Higher Education of China
  • 20114407110006
the Science and Technology Program of Guangzhou, China
  • 2014J4100053
the Scientific Research Foundation of Graduate School of South China Normal University
  • 2012kyjj114

Copyright information

© Springer International Publishing Switzerland 2015

Authors and Affiliations

  • Rong Qin
    • 1
  • Chanjuan Ning
    • 1
  • Lars O. Björn
    • 1
    • 2
  • Shaoshan Li
    • 1
  1. 1.Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education, School of Life ScienceSouth China Normal UniversityGuangzhouChina
  2. 2.Department of Biology, Molecular Cell BiologyLund UniversityLundSweden

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