Mapping QTL associated with remobilization of zinc from vegetative tissues into grains of barley (Hordeum vulgare)
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Background and aims
Limited remobilization of Zn from vegetative tissues into grains via phloem is a major physiological barrier against Zn loading into cereal grains. In present experiment, doubled-haploid mapping population (150 lines, derived from Clipper ×Sahara) of barley was genetically characterized for differential Zn remobilization.
The germplasm was grown under glasshouse conditions. Leaves (upper three), stem (the rest of the plant) and mature grains were sampled from the main-tillers at anthesis and maturity for Zn analysis. Quantitative trait loci (QTL) regulating time to anthesis, plant biomass, Zn concentration in vegetative tissues and remobilization of Zn from these tissues into grains were identified using a genetic linkage map of 485 markers.
A significant variation existed in grain Zn concentration among the lines (27–75 μg Zn g−1), and it correlated with the amount of Zn remobilized from vegetative tissues into grains. Sahara remobilized 37 % of pre-anthesis Zn reserves into grains; the presence of its alleles at all QTL associated with leaf (3 QTL) and stem (2 QTL) Zn remobilization increased the trait score.
Present study provided an insight into the genetic basis of Zn remobilization from vegetative tissues into barley grains. Such information is useful in breeding for Zn biofortification.
KeywordsBarley Biofortification Clipper × Sahara Quantitative trait loci Zinc remobilization
The research project was financially supported by Higher Education Commission of Pakistan, International Plant Nutrition Institute (USA) and Australian Research Council. We are also thankful to Messrs. Michael Smirk and Paul Damon, School of Earth and Environment, The University of Western Australia, for their help with preparation of plant samples and Zn analysis.
Conflict of interest
Authors declare no conflict of interest.
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