Plant and Soil

, Volume 398, Issue 1–2, pp 301–312 | Cite as

Transcriptional response of plasma membrane H+-ATPase genes to ammonium nutrition and its functional link to the release of biological nitrification inhibitors from sorghum roots

  • Houqing Zeng
  • Tingjun Di
  • Yiyong Zhu
  • Guntur Venkata Subbarao
Regular Article
First Online:
Received:
Accepted:

Abstract

Aims

Sorghum (Sorghum bicolor) roots release biological nitrification inhibitors (BNIs) to suppress soil nitrification. Presence of NH4 + in the rhizosphere stimulates BNIs release and it is hypothesized to be functionally associated with plasma membrane (PM) H+-ATPase activity. However, whether the H+-ATPase is regulated at the transcriptional level, and if so, which isoforms of the H+-ATPases are involved in BNIs release are not known. Also, it is not clear whether the stimulation on BNIs release from roots is due to NH4 + uptake or its assimilation, which are addressed in this study.

Methods

Root exudates from intact sorghum plants were collected using aerated solutions of NH4 + or methyl-ammonium (MeA); and the BNI-activity release was determined. PM vesicles were isolated from fresh roots using a two-phase partitioning system; and the hydrolytic H+-ATPase activity was determined. All genes encoding PM H+-ATPases were searched in sorghum genome, and their expression in response to NH4 + or MeA were analyzed by quantitative RT-PCR in sorghum roots.

Results

BNIs release and PM H+-ATPase activity increased with NH4 + concentration (≤1.0 mM) in the root-exudate collection solutions, but at higher concentrations, it did not respond further or declined in case of the PM H+-ATPase activity. Twelve PM H+-ATPase genes were identified in sorghum genome; and these isoforms were designated SbA1 to SbA12. Five H+-ATPase genes were stimulated by NH4 + in the rhizosphere, and have similar expression pattern, which is consistent with the variation in H+-ATPase activity. MeA, a non-metabolizable analogue of NH4 +, had no significant effects on BNIs release, H+-ATPase activity, or expression of the H+-ATPase genes.

Conclusions

Our results suggest that the functional link between PM H+-ATPase activity and BNIs release is evident only at NH4 + levels of ≤1.0 mM in the rhizosphere. The variation in PM H+-ATPase activity by NH4 + is due to transcriptional regulation of five isoforms of the H+-ATPases. The stimulatory effect of NH4 + on BNIs release is functionally associated with NH4 + assimilation and not just with NH4 + uptake alone.

Keywords

Plasma membrane H+-ATPase Biological nitrification inhibition (BNI) Biological nitrification inhibitors (BNIs) Ammonium Transcriptional regulation Sorghum (Sorghum bicolor) 
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Notes

Acknowledgments

The research presented here is supported through JIRCAS invitation fellowship program to co-authors (Drs. Houqing Zeng, Tingjun Di and Prof. Yiyong Zhu), and is funded by grant-in-Aid for scientific research from Ministry of Agriculture, Forestry and Fisheries of Japan (MAFF) to JIRCAS under BNI project. Funding support also came from Natural Science Foundation of China (NSFC 31172035) and Program of New Century Excellent Talent in Universities (NCET-11-0672).

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Copyright information

© Springer International Publishing Switzerland 2015

Authors and Affiliations

  • Houqing Zeng
    • 1
    • 2
  • Tingjun Di
    • 1
  • Yiyong Zhu
    • 3
  • Guntur Venkata Subbarao
    • 1
  1. 1.Crop, Livestock & Environment DivisionJIRCASTsukubaJapan
  2. 2.College of Life and Environmental SciencesHangzhou Normal UniversityHangzhouPeople’s Republic of China
  3. 3.College of Resource and Environmental SciencesNanjing Agricultural UniversityNanjingPeople’s Republic of China

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