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Plant and Soil

, Volume 398, Issue 1–2, pp 59–77 | Cite as

Measurements of water uptake of maize roots: the key function of lateral roots

  • Mutez A. AhmedEmail author
  • Mohsen Zarebanadkouki
  • Anders Kaestner
  • Andrea Carminati
Regular Article

Abstract

Aims

Maize (Zea mays L.) is one of the most important crops worldwide. Despite several studies on maize roots, there is limited information on the function of different root types in extracting water from soils. Aim of this study was to investigate the location of water uptake in maize roots.

Methods

We used neutron radiography to image the spatial distribution of maize roots in soil and trace the transport of deuterated water (D2O) in soil and roots. Maize plants were grown in aluminum containers filled with a sandy soil that was kept homogeneously wet throughout the experiment. When the plants were 16 days old, we injected D2O into selected soil regions. The transport of D2O was simulated using a diffusion–convection numerical model. By fitting the observed D2O transport we quantified the diffusion coefficient and the water uptake of the different root segments.

Results

The root architecture of a 16 day-old maize consisted of a primary root, 4–5 seminal roots and many lateral roots. Laterals emerged from the proximal 15 cm of the primary and seminal roots. During both day and night measurements, D2O entered more quickly into lateral roots than into primary and seminal roots. The quick transport of D2O into laterals was caused by the small radius of lateral roots. The diffusion coefficient of lateral roots (4.68 × 10−7 cm2 s−1) was similar to that of the distal unbranched segments of seminal roots (4.72 × 10−7 cm2 s−1) and higher than that of the proximal branched segments (1.42 × 10−7 cm2 s−1). Water uptake of lateral roots (1.64 × 10−5 cm s−1) was much higher than the uptake of seminal roots, which was 5.34 × 10−10 cm s−1 in the proximal branched segments and only 1.18 × 10−12 cm s−1 in the distal unbranched segments.

Conclusions

We conclude that the function of lateral roots is to absorb water from the soil, while the function of the primary and seminal roots is to axially transport water to the shoot.

Keywords

Lateral roots Seminal roots Neutron radiography Root water uptake Deuterated water (D2O) Maize Radial and axial conductivity 

Notes

Acknowledgments

The doctoral position of Mutez Ahmed was funded by the German Academic Exchange Service (DAAD). We are grateful to the staff at the ICON imaging station of the Paul Scherrer Institute (PSI), Villigen, Switzerland for their technical support during the measurements with neutron radiography. KWS is appreciated for providing Maize seeds. Finally, we would like to thank Claude Doussan for his comments on a former presentation of this study and two anonymous reviewers for the constructive comments on the former version of the manuscript.

Supplementary material

11104_2015_2639_MOESM1_ESM.docx (5.2 mb)
ESM 1 (DOCX 5367 kb)

References

  1. Ahmed MA, Kroener E, Holz M et al (2014) Mucilage exudation facilitates root water uptake in dry soils. Funct Plant Biol 41:1129–1137CrossRefGoogle Scholar
  2. Aubin GS, Canny MJ, McCully ME (1986) Living vessel elements in the late metaxylem of sheathed maize roots. Ann Bot 58:577–588. doi: 10.1093/annbot/58.4.577 CrossRefGoogle Scholar
  3. Bramley H, Turner NC, Turner DW, Tyerman SD (2009) Roles of morphology, anatomy, and aquaporins in determining contrasting hydraulic behavior of roots. Plant Physiol 150:348–364. doi: 10.1104/pp. 108.134098 CrossRefPubMedPubMedCentralGoogle Scholar
  4. Brodersen CR (2013) Visualizing water transport in roots: advanced imaging tools for an expanding field. Plant Soil 366:29–32. doi: 10.1007/s11104-013-1657-5 CrossRefGoogle Scholar
  5. Brouwer R (1953) Water absorption by the roots of vicia faba at various transpiration strength: analysis of the uptake and the factors determining it. I.Google Scholar
  6. Carminati A (2012) A model of root water uptake coupled with rhizosphere dynamics. Vadose Zone J 11. doi:  10.2136/vzj2011.0106
  7. Carminati A, Moradi AB, Vetterlein D et al (2010) Dynamics of soil water content in the rhizosphere. Plant Soil 332:163–176. doi: 10.1007/s11104-010-0283-8 CrossRefGoogle Scholar
  8. Carminati A, Schneider CL, Moradi AB et al (2011) How the rhizosphere May favor water availability to roots. Vadose Zone J 10:988. doi: 10.2136/vzj2010.0113 CrossRefGoogle Scholar
  9. Clarkson DT, Carvajal M, Henzler T et al (2000) Root hydraulic conductance: diurnal aquaporin expression and the effects of nutrient stress. J Exp Bot 51:61–70. doi: 10.1093/jexbot/51.342.61 CrossRefPubMedGoogle Scholar
  10. Da Ines O, Graf W, Franck KI et al (2010) Kinetic analyses of plant water relocation using deuterium as tracer – reduced water flux of Arabidopsis pip2 aquaporin knockout mutants. Plant Biol 12:129–139. doi: 10.1111/j.1438-8677.2010.00385.x CrossRefPubMedGoogle Scholar
  11. Doussan C, Pagès L, Vercambre G (1998) Modelling of the hydraulic architecture of root systems: an integrated approach to water absorption—model description. Ann Bot 81:213–223. doi: 10.1006/anbo.1997.0540 CrossRefGoogle Scholar
  12. Doussan C, Pierret A, Garrigues E, Pagès L (2006) Water uptake by plant roots: II – modelling of water transfer in the soil root-system with explicit account of flow within the root system – comparison with experiments. Plant Soil 283:99–117. doi: 10.1007/s11104-004-7904-z CrossRefGoogle Scholar
  13. Frensch J, Steudle E (1989) Axial and radial hydraulic resistance to roots of maize (Zea mays L.). Plant Physiol 91:719–726CrossRefPubMedPubMedCentralGoogle Scholar
  14. Garrigues E, Doussan C, Pierret A (2006) Water uptake by plant roots: I – formation and propagation of a water extraction front in mature root systems as evidenced by 2D light transmission imaging. Plant Soil 283:83–98. doi: 10.1007/s11104-004-7903-0 CrossRefGoogle Scholar
  15. Hochholdinger F (2009) Handbook of Maize: Its Biology. In: Bennetzen JL, Hake SC (eds) The Maize Root System: Morphology, Anatomy, and Genetics. Springer, New York, pp 145–160Google Scholar
  16. Hochholdinger F, Park WJ, Sauer M, Woll K (2004a) From weeds to crops: genetic analysis of root development in cereals. Trends Plant Sci 9:42–48. doi: 10.1016/j.tplants.2003.11.003 CrossRefPubMedGoogle Scholar
  17. Hochholdinger F, Woll K, Sauer M, Dembinsky D (2004b) Genetic dissection of root formation in maize (Zea mays) reveals root-type specific developmental programmes. Ann Bot 93:359–368. doi: 10.1093/aob/mch056 CrossRefPubMedPubMedCentralGoogle Scholar
  18. Javaux M, Schröder T, Vanderborght J, Vereecken H (2008) Use of a three-dimensional detailed modeling approach for predicting root water uptake. Vadose Zone J 7:1079. doi: 10.2136/vzj2007.0115 CrossRefGoogle Scholar
  19. Koebernick N, Weller U, Huber K, et al (2014) In situ visualization and quantification of three-dimensional root system architecture and growth using x-ray computed tomography. Vadose Zone J 13. doi:  10.2136/vzj2014.03.0024
  20. Landsberg JJ, Fowkes ND (1978) Water movement through plant roots. Ann Bot 42:493–508. doi: 10.1093/oxfordjournals.aob.a085488 CrossRefGoogle Scholar
  21. Lynch J (1995) Root architecture and plant productivity. Plant Physiol 109:7–13CrossRefPubMedPubMedCentralGoogle Scholar
  22. Lynch JP, Chimungu JG, Brown KM (2014) Root anatomical phenes associated with water acquisition from drying soil: targets for crop improvement. J Exp Bot. doi: 10.1093/jxb/eru162 PubMedGoogle Scholar
  23. Marris E (2008) Water: more crop per drop. Nat New 452:273–277. doi: 10.1038/452273a CrossRefGoogle Scholar
  24. Matsushima U, Kardjilov N, Hilger A et al (2012) Application potential of cold neutron radiography in plant science research. J Appl Bot Food Qual 82:90–98Google Scholar
  25. Maurel C, Verdoucq L, Luu D-T, Santoni V (2008) Plant aquaporins: membrane channels with multiple integrated functions. Annu Rev Plant Biol 59:595–624. doi: 10.1146/annurev.arplant.59.032607.092734 CrossRefPubMedGoogle Scholar
  26. McCully ME (1999) Roots in soil: unearthing the complexities of roots and their rhizospheres. Annu Rev Plant Physiol Plant Mol Biol 50:695–718. doi: 10.1146/annurev.arplant.50.1.695 CrossRefPubMedGoogle Scholar
  27. McCully ME, Canny MJ (1988) Pathways and processes of water and nutrient movement in roots. Plant Soil 111:159–170. doi: 10.1007/BF02139932 CrossRefGoogle Scholar
  28. Menon M, Robinson B, Oswald SE et al (2007) Visualization of root growth in heterogeneously contaminated soil using neutron radiography. Eur J Soil Sci 58:802–810. doi: 10.1111/j.1365-2389.2006.00870.x CrossRefGoogle Scholar
  29. Moradi AB, Conesa HM, Robinson B et al (2008) Neutron radiography as a tool for revealing root development in soil: capabilities and limitations. Plant Soil 318:243–255. doi: 10.1007/s11104-008-9834-7 CrossRefGoogle Scholar
  30. Moradi AB, Carminati A, Vetterlein D et al (2011) Three-dimensional visualization and quantification of water content in the rhizosphere. New Phytol 192:653–663. doi: 10.1111/j.1469-8137.2011.03826.x CrossRefPubMedGoogle Scholar
  31. Morison JIL, Baker NR, Mullineaux PM, Davies WJ (2008) Improving water use in crop production. Philos Trans R Soc B Biol Sci 363:639–658. doi: 10.1098/rstb.2007.2175 CrossRefGoogle Scholar
  32. North GB, Nobel PS (1997) Drought-induced changes in soil contact and hydraulic conductivity for roots of Opuntia ficus-indica with and without rhizosheaths. Plant Soil 191:249–258CrossRefGoogle Scholar
  33. Ordin L, Kramer PJ (1956) Permeability of vicia faba root segments to water as measured by diffusion of deuterium hydroxide. 12. Plant Physiol 31:468–471CrossRefPubMedPubMedCentralGoogle Scholar
  34. Oswald SE, Menon M, Carminati A et al (2008) Quantitative imaging of infiltration, root growth, and root water uptake via neutron radiography. Vadose Zone J 7:1035. doi: 10.2136/vzj2007.0156 CrossRefGoogle Scholar
  35. Parry MAJ, Hawkesford MJ (2010) Food security: increasing yield and improving resource use efficiency. Proc Nutr Soc 69:592–600. doi: 10.1017/S0029665110003836 CrossRefPubMedGoogle Scholar
  36. Pohlmeier A, Javaux M, Vereecken H, et al (2013) Magnetic Resonance Imaging Techniques for Visualization of Root Growth and Root Water Uptake Processes. In: SSSA Special Publication. The Soil Science Society of America, Inc.Google Scholar
  37. Rewald B, Ephrath JE, Rachmilevitch S (2011) A root is a root is a root? Water uptake rates of citrus root orders. Plant Cell Environ 34:33–42. doi: 10.1111/j.1365-3040.2010.02223.x CrossRefPubMedGoogle Scholar
  38. Sanderson J (1983) Water uptake by different regions of the barley root. Pathways of radial flow in relation to development of the endodermis. J Exp Bot 34:240–253. doi: 10.1093/jxb/34.3.240 CrossRefGoogle Scholar
  39. Sierp H, Brewig A (1935) Quantitative untersuchungen über die wasserabsorptionszone der wurzeln. Jahrb Wiss Bot 82:99–122Google Scholar
  40. Sposito G (2013) Green water and global food security. Vadose Zone J 12. doi:  10.2136/vzj2013.02.0041
  41. Steudle E (2000) Water uptake by plant roots: an integration of views. Plant Soil 226:45–56CrossRefGoogle Scholar
  42. Tumlinson LG, Liu H, Silk WK, Hopmans JW (2008) Thermal neutron computed tomography of soil water and plant roots. Soil Sci Soc Am J 72:1234. doi: 10.2136/sssaj2007.0302 CrossRefGoogle Scholar
  43. Varney GT, Canny MJ (1993) Rates of water uptake into the mature root system of maize plants. New Phytol 775–786Google Scholar
  44. Wallace JS (2000) Increasing agricultural water use efficiency to meet future food production. Agric Ecosyst Environ 82:105–119. doi: 10.1016/S0167-8809(00)00220-6 CrossRefGoogle Scholar
  45. Wang X-L, Canny MJ, McCully ME (1991) The water status of the roots of soil-grown maize in relation to the maturity of their xylem. Physiol Plant 82:157–162. doi: 10.1111/j.1399-3054.1991.tb00075.x CrossRefGoogle Scholar
  46. Warren JM, Bilheux H, Kang M et al (2013) Neutron imaging reveals internal plant water dynamics. Plant Soil 366:683–693. doi: 10.1007/s11104-012-1579-7 CrossRefGoogle Scholar
  47. Watt M, McCully ME, Canny MJ (1994) Formation and stabilization of rhizosheaths of Zea mays L. (Effect of soil water content). Plant Physiol 106:179–186CrossRefPubMedPubMedCentralGoogle Scholar
  48. Zarebanadkouki M, Carminati A (2014) Reduced root water uptake after drying and rewetting. J Plant Nutr Soil Sci 177:227–236. doi: 10.1002/jpln.201300249 CrossRefGoogle Scholar
  49. Zarebanadkouki M, Kim YX, Moradi AB, et al (2012) Quantification and modeling of local root water uptake using neutron radiography and deuterated water. Vadose Zone J 11. doi:  10.2136/vzj2011.0196
  50. Zarebanadkouki M, Kim YX, Carminati A (2013) Where do roots take up water? Neutron radiography of water flow into the roots of transpiring plants growing in soil. New Phytol 199:1034–1044. doi: 10.1111/nph.12330 CrossRefPubMedGoogle Scholar
  51. Zarebanadkouki M, Kroener E, Kaestner A, Carminati A (2014) Visualization of root water uptake: Quantification of deuterated water transport in roots using neutron radiography and numerical modeling. Plant Physiol 114.243212. doi:  10.1104/pp.114.243212
  52. Zwieniecki MA, Thompson MV, Holbrook NM (2003) Understanding the hydraulics of porous pipes: tradeoffs between water uptake and root length utilization. J Plant Growth Regul 21:315–323. doi: 10.1007/s00344-003-0008-9 CrossRefGoogle Scholar

Copyright information

© Springer International Publishing Switzerland 2015

Authors and Affiliations

  • Mutez A. Ahmed
    • 1
    • 3
    Email author
  • Mohsen Zarebanadkouki
    • 1
  • Anders Kaestner
    • 2
  • Andrea Carminati
    • 1
  1. 1.Division of Soil HydrologyGeorg-August University of GöttingenGöttingenGermany
  2. 2.Paul Scherrer InstituteVilligenSwitzerland
  3. 3.Department of Agricultural Engineering, Faculty of AgricultureUniversity of KhartoumKhartoum NorthSudan

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