Advertisement

Plant and Soil

, Volume 386, Issue 1–2, pp 65–76 | Cite as

Root plasticity and its functional roles were triggered by water deficit but not by the resulting changes in the forms of soil N in rice

  • Thiem Thi Tran
  • Mana Kano-Nakata
  • Roel Rodriguez Suralta
  • Daniel Menge
  • Shiro Mitsuya
  • Yoshiaki Inukai
  • Akira Yamauchi
Regular Article

Abstract

Background

The functional roles of root plasticity in rice adaptation to drought conditions may vary with soil nitrogen (N) conditions.

Aims

To examine if: promoted root system plastic development triggered by mild drought stress and enhanced by N application would contribute to the increase in soil water uptake, and if expression of root system plasticity would be affected by different forms of N applied into the soil.

Methods

Chromosome segment substitution line (CSSL) 50 and Nipponbare genotypes were grown under continuously waterlogged (CWL) and water deficit (WD) conditions. In rootbox (25 cm × 40 cm × 2 cm) experiment, three fertilizer N levels; (30 (low), 60 (standard) and 120 mg N (high) per rootbox) were used while in pot (5 L) experiment, six N forms (NH4 +-N alone, NO3 -N alone, combined NH4 +-N and NO3 -N with and without dicyandiamide (nitrification inhibitor) were used at the rate of 360 mg N per pot.

Results

In both experiments, CSSL50 and Nipponbare had no significant differences in shoot and root growth regardless of N levels and N forms under CWL conditions. However, under WD conditions, CSSL50 had significantly greater dry matter production (DMP) than Nipponbare due to the greater ability of the former for maintaining soil water uptake and photosynthesis. The observed higher water uptake and photosynthesis in CSSL50 under WD was closely related to its promoted root system development due to plasticity, which were significantly greater at high N than at low N level. The extent of promotion in root system development based total root length was not significantly different among N forms.

Conclusions

The root system plasticity of CSSL50 in response to WD was expressed at a greater degree with high level of N applied and the functional roles of root plasticity for greater soil water uptake and DMP were due to WD regardless of N forms.

Keywords

Ammonium Chromosome segment substitution lines Dry matter production Nitrate Root plasticity Water deficit 

Abbreviations

CSSLs

chromosome segment substitution lines

DAS

days after sowing

SMC

soil moisture content

CWL

continuously waterlogged

WD

water deficit

N

nitrogen

G

genotype

DMP

dry matter production

Notes

Acknowledgments

We thank Dr. Jonathan M. Niones of the Philippine Rice Research Institute for a critical review and useful comments on our manuscript. This research was funded by the Grant-in-Aid for Scientific Research (No.22405042) from the Japan Society for the Promotion of Science, and partially supported by the Japan Science and Technology Agency (JST)/Japan International Cooperation Agency (JICA), the Science and Technology Research Partnership for Sustainable Development (SATREPS).

Supplementary material

11104_2014_2240_MOESM1_ESM.doc (62 kb)
ESM 1 (DOC 62 kb)

References

  1. Allahyar F (2011) Interaction effects of nitrogen and irrigation methods on the growth and yield of rice in Amol area. Intl J Agri Crop Sci 3–4:111–113Google Scholar
  2. Aulakh MS, Khera TS, Doran JW (2000) Mineralization and denitrification in upland, nearly saturated and flooded subtropical soil. I. Effect of nitrate and ammoniacal nitrogen. Biol Fertil Soils 31:162–167CrossRefGoogle Scholar
  3. Bañoc DM, Yamauchi A, Kamoshita A, Wade LJ, Pardales JR Jr (2000) Genotypic variations in response of lateral root development to fluctuating soil moisture in rice. Plant Prod Sci 3:335–343CrossRefGoogle Scholar
  4. Belder P, Bouman BAM, Spiertz JHJ, Peng S, Castañeda AR, Visperas RM (2005) Crop performance, nitrogen and water use in flooded and aerobic rice. Plant Soil 273:167–182CrossRefGoogle Scholar
  5. Blum A (2005) Drought resistance, water use efficiency and yield potential are they compatible, dissonant and mutually exclusive? Aust J Agr Res 56:1159–1168CrossRefGoogle Scholar
  6. Bolan NS, Saggar S, Singh J (2004) The role of inhibitors in mitigating nitrogen losses in grazed pasture. N Z Soil News 42Google Scholar
  7. Castillo EG, Tuong TP, Singh U, Inubushi K, Padilla J (2006) Drought response of dry-seeded rice to water stress timing and N-fertilizer rates and sources. Soil Sci Plant Nutr 52:496–508CrossRefGoogle Scholar
  8. Dorlodot S, Forster B, Pagès L, Price A, Tuberosa R, Draye X (2007) Root system architecture: opportunities and constraints for genetic improvement of crops. Trends Plant Sci 12:474–81PubMedCrossRefGoogle Scholar
  9. Fageria NK (2010) Root growth of upland rice genotypes as influenced by nitrogen fertilization. 19th World Congress of Soil Science, Soil Solutions for a Changing World, Australia, pp 120–122Google Scholar
  10. Gao Y, Li Y, Yang X, Shen Q, Li H, Guo S (2010) Ammonium nutrition increases water absorption in rice seedlings (Oryza sativa L.) under water stress. Plant Soil 331:193–201CrossRefGoogle Scholar
  11. Gowda VRP, Henry A, Yamauchi A, Shashidhar HE, Serraj R (2011) Root biology and genetic improvement for drought avoidance in rice. Field Crop Res 122:1–13CrossRefGoogle Scholar
  12. Guo S, Kaldenhoff R, Uehlein N, Sattelmacher B, Brueck H (2007) Relationship between water and nitrogen uptake in nitrate- and ammonium-supplied Phaseolus vulgaris L. plants. J Plant Nutr Soil Sci 170:73–80CrossRefGoogle Scholar
  13. IRRI (2009) CROPSTAT Version 7.2. Metro Manila: International Rice Research Institute. Available at http://archive.irri.org/science/software/cropstat.asp
  14. Kamoshita A, Rodriguez R, Yamauchi A, Wade LJ (2004) Genotypic varia- tion in response of rainfed lowland rice to prolonged drought and rewatering. Plant Prod Sci 7:406–420CrossRefGoogle Scholar
  15. Kamoshita A, Wade LJ, Yamauchi A (2000) Genotypic variation in response of rainfed lowland rice to drought and rewatering. III. Water extraction during the drought period. Plant Prod Sci 3:189–196CrossRefGoogle Scholar
  16. Kano M, Inukai Y, Kitano H, Yamauchi A (2011) Root plasticity as the key root trait for adaptation to various intensities of drought stress in rice. Plant Soil 342:117–128CrossRefGoogle Scholar
  17. Kano-Nakata M, Inukai Y, Wade LJ, Siopongco JDLC, Yamauchi A (2011) Root development and water uptake, and shoot dry matter production under water deficit conditions in two CSSLs of rice: functional roles of root plasticity. Plant Prod Sci 14:307–317CrossRefGoogle Scholar
  18. Kano-Nakata M, Suralta RR, Niones JM, Yamauchi A (2012) Root sampling by using a root box–pinboard method. In: Shashidhar HE, Henry A, Hardy B (eds) Methodologies for root drought studies in rice. Los Baños (Philippines), International Rice Research Institute, pp 3–8Google Scholar
  19. Kano-Nakata M, Gowda VRP, Henry A, Serraj R, Inukai Y, Fujita D, Kobayashi N, Suralta RR, Yamauchi A (2013) Functional roles of the plasticity of root system development in biomass production and water uptake under rainfed lowland conditions. Field Crop Res 144:288–296CrossRefGoogle Scholar
  20. Kato Y, Kamoshita A, Yamagishi J, Imoto H, Abe J (2007) Growth of rice (Oryza sativa L.) cultivars under upland conditions with different levels of water supply. Plant Prod Sci 10:3–13CrossRefGoogle Scholar
  21. Kondo M, Sing CV, Agbisit R, Murty MVR (2005) Yield response to urea and controlled-release urea as affected by water supply in tropical upland rice. J Plant Nutr 28:201–219CrossRefGoogle Scholar
  22. Kono Y, Yamauchi A, Nonoyama T, Tatsumi J, Kawamura N (1987) A revised experimental system of root-soil interaction for laboratory work. Environ Contor Biol 25:141–151Google Scholar
  23. Landi P, Giuliani S, Salvi S, Ferri M, Tuberosa R, Sanguineti MC (2010) Characterization of root-yield-1.06, a major constitutive QTL for root and agronomic traits in maize across water regimes. J Exp Bot 61:3553–3562PubMedCrossRefGoogle Scholar
  24. Laperche A, Devienne-Barret F, Maury O, Le Gouis J, Ney B (2006) A simplified conceptual model of carbon/nitrogen functioning for QTL analysis of winter wheat adaptation to nitrogen deficiency. Theor Appl Genet 113:1131–1146PubMedCrossRefGoogle Scholar
  25. Li Y, Gao Y, Ding L, Shen Q, Guo S (2009) Ammonium enhances the tolerance of rice seedlings (Oryza sativa L.) to drought condition. Agr Water Manag 96:1746–1750CrossRefGoogle Scholar
  26. Nevo E, Chen G (2010) Drought and salt tolerances in wild relatives for wheat and barley improvement. Plant Cell Environ 33:670–685. doi: 10.1111/j.1365-3040.2009.02107.x PubMedCrossRefGoogle Scholar
  27. Niones JM, Suralta RR, Inukai Y, Yamauchi A (2012) Field evaluation functional roles of root plastic responses on dry matter production and grain yield of rice under cycles of transient soil moisture stresses using chromosome segment substitution lines. Plant Soil 359:107–120CrossRefGoogle Scholar
  28. Niones JM, Suralta RR, Inukai Y, Yamauchi A (2013) Role of root aerenchyma development and its associated QTL in dry matter production under transient moisture stress in rice. Plant Prod Sci 16:205–216CrossRefGoogle Scholar
  29. Obara M, Tamura W, Ebitani T, Yano M, Sato T, Yamaya T (2010) Fine-mapping of qRL6.1, a major QTL for root length of rice seedlings grown under a wide range of NH4 + concentrations in hydroponic conditions. Theor Appl Genet 121:535–547PubMedCentralPubMedCrossRefGoogle Scholar
  30. Otoo E, Ishii R, Kumura A (1989) Interaction of nitrogen supply and soil water stress on photosynthesis and transpiration in rice. Jpn J Crop Sci 58:424–429CrossRefGoogle Scholar
  31. Price AH, Steele KA, Moore BJ, Barraclough PB, Clark LJ (2000) A combined RFLP and AFLP linkage map of upland rice (Oryza sativa L.) used to identify QTLs for root-penetration ability. Theor Appl Gen 100:49–56CrossRefGoogle Scholar
  32. Qian X, Shen Q, Xu G, Wang J, Zhou M (2004) Nitrogen Form Effects on Yield and Nitrogen Uptake of Rice Crop Grown in Aerobic Soil. J Plant Nutr 27:1061–1076CrossRefGoogle Scholar
  33. Ruta N, Liedgens M, Fracheboud Y, Stamp P, Hund A (2010) QTLs for the elongation of axile and lateral roots of maize in response to low water potential. Theor Appl Genet 120:621–631PubMedCrossRefGoogle Scholar
  34. Serraj R, Krishnamurthy L, Kashiwagi J, Kumar J, Chandra S, Crouch JH (2004) Variation in root traits of chickpea (Cicer arietinum L.) grown under terminal drought. Field Crop Res 88:115–127CrossRefGoogle Scholar
  35. Serraj R, Kumar A, McNally KL, Slamet-Loedin I, Bruskiewich R, Mauleon R, Cairns J, Hijmans RJ (2009) Improvement of drought resistance in rice. Adv Agron 103:41–99CrossRefGoogle Scholar
  36. Sharma S, Xu S, Ehdaie B, Hoops A, Close TJ, Liukaszewskie AJ, Giles Waines J (2011) Dissection of QTL effects for root traits using a chromosome arm-specific mapping population in bread wheat. Theor Appl Genet 122:759–769PubMedCentralPubMedCrossRefGoogle Scholar
  37. Siopongco JDLC, Yamauchi A, Salekdeh H, Bennett J, Wade LJ (2005) Root growth and water extraction responses of double haploid rice lines to drought and rewatering during the vegetative stage. Plant Prod Sci 9:141–151CrossRefGoogle Scholar
  38. Siopongco JDLC, Yamauchi A, Salekdeh H, Bennett J, Wade LJ (2006) Growth and water use response of doubled haploid rice lines to drought and rewatering during the vegetative stage. Plant Prod Sci 9:141–151CrossRefGoogle Scholar
  39. Siopongco JDLC, Sekiya K, Yamauchi A, Egdane J, Ismail AM, Wade LJ (2008) Stomatal responses in rainfed lowland rice to partial soil drying; evidence of root signals. Plant Prod Sci 11:28–41CrossRefGoogle Scholar
  40. Song W, Makeen K, Wang D, Zhang C, Xu Y, Zhao H, Tu E, Zhang Y, Shen Q, Xu G (2011) Nitrate supply affects root growth differentially in two rice cultivars differing in nitrogen use efficiency. Plant Soil 343:357–368Google Scholar
  41. Suralta RR (2010) Plastic root system development responses to drought-enhanced nitrogen uptake during progressive soil drying conditions in rice. Philipp Agr Sci 93:458–462Google Scholar
  42. Suralta RR, Inukai Y, Yamauchi A (2010) Dry matter production in relation to root plastic development. Oxygen transport, and water upatake of rice under transient soil moisture stresses. Plant Soil 332:87–104CrossRefGoogle Scholar
  43. Tran TT, Kano-Nakata M, Takade M, Menge D, Mitsuya S, Inukai Y, Yamauchi A (2014) Nitrogen application enhanced the expression of developmental plasticity of root system triggered by mild drought stress in rice. Plant Soil. doi: 10.1007/s11104-013-2013-5 Google Scholar
  44. Uga Y, Okuno K, Yano M (2011) Dro1, a major QTL involved in deep rooting of rice under upland field conditions. J Exp Bot 62:2485–2494PubMedCrossRefGoogle Scholar
  45. Wade LJ, Fukai S, Samson BK, Ali A, Mazid MA (1999) Rainfed lowland rice: physical environment and cultivar requirements. Field Crop Res 64:3–12CrossRefGoogle Scholar
  46. Wang H, Yamauchi A (2006) Growth and function of roots under abiotic stress soils. In: Huang B (ed) Plant environment interactions, 3rd edn. CRC Press, Taylor and Francis Group, LLC, New York, pp 271–320CrossRefGoogle Scholar
  47. Weatherburn M (1967) Phenol-hypochlorite reaction for determination of ammonia. Anal Chem 39:971–974CrossRefGoogle Scholar
  48. Yadav RS, Sehgal D, Vadez V (2011) Using genetic mapping and genomics approaches in understanding and improving drought tolerance in pearl millet. J Exp Bot 62:397–408PubMedCrossRefGoogle Scholar
  49. Yamauchi A, Kono Y, Tatsumi J (1988) Comparative growth analysis of upland rice and maize grown under different soil moisture conditions. Jpn J Crop Sci 57:174–183CrossRefGoogle Scholar
  50. Yamauchi A, Pardales JR Jr, Kono Y (1996) Root system structure and its relation to stress tolerance. In: Ito O, Katayama K, Johansen C, Kumar Rao JVDK, Adu-Gyamfi JJ, Rego TJ (eds) Roots and nitrogen in cropping systems of the semi-arid tropics. JIRCAS Publication, Tsukuba, Japan, pp 211–234Google Scholar
  51. Yang X, Li Y, Ren B, Ding L, Gao C, Shen Q, Guo S (2012) Drought-induced root aerenchyma formation restricts water uptake in rice seedlings supplied with nitrate. Plant Cell Physiol 53:495–504PubMedCrossRefGoogle Scholar

Copyright information

© Springer International Publishing Switzerland 2014

Authors and Affiliations

  • Thiem Thi Tran
    • 1
    • 2
  • Mana Kano-Nakata
    • 3
  • Roel Rodriguez Suralta
    • 1
    • 4
  • Daniel Menge
    • 1
  • Shiro Mitsuya
    • 1
  • Yoshiaki Inukai
    • 3
  • Akira Yamauchi
    • 1
  1. 1.Graduate School of Bioagricultural SciencesNagoya UniversityNagoyaJapan
  2. 2.Faculty of AgronomyVietnam National University of AgricultureHanoiVietnam
  3. 3.International Cooperation Center for Agricultural EducationNagoya UniversityNagoyaJapan
  4. 4.Agronomy, Soils and Plant Physiology DivisionPhilippine Rice Research Institute (PhilRice), Maligaya, Science City of MuñozNueva EcijaPhilippines

Personalised recommendations