As, Pb, Sb, and Zn transfer from soil to root of wild rosemary: do native symbionts matter?
- 487 Downloads
Background and aims
This is an in natura study aimed to determine the potential of Rosmarinus officinalis for phytostabilization of trace metal and metalloid (TMM)-contaminated soils in the Calanques National Park (Marseille, southeast of France). The link between rosemary tolerance/accumulation of As, Pb, Sb, and Zn and root symbioses with arbuscular mycorrhizal (AM) fungi and/or dark septate endophytes (DSE) was examined.
Eight sites along a gradient of contamination were selected for soil and root collections. TMM concentrations were analyzed in all the samples and root symbioses were observed. Moreover, in the roots of various diameters collected in the most contaminated site, X-ray microfluorescence methods were used to determine TMM localization in tissues.
Rosemary accumulated, in its roots, the most labile TMM fraction in the soil. The positive linear correlation between TMM concentrations in soil and endophyte root colonization rates suggests the involvement of AM fungi and DSE in rosemary tolerance to TMM. Moreover, a typical TMM localization in root peripheral tissues of thin roots containing endophytes forming AM and DSE development was observed using X-ray microfluorescence.
Rosemary and its root symbioses appeared as a potential candidate for a phytostabilization process of metal-contaminated soils in Mediterranean area.
KeywordsTrace metals and metalloid multicontamination Arbuscular mycorrhizal fungi Dark septate endophytes Phytostabilization μXRF analyses
Trace metals and metalloids
Dark septate endophytes
Pollution load index
- Affholder MC, Prudent P, Masotti V, Coulomb B, Rabier J, Nguyen-The B, Laffont-Schwob I (2013) Transfer of metals and metalloids from soil to shoots in wild rosemary (Rosmarinus officinalis L.) growing on a former lead smelter site: Human exposure risk. Sci Total Environ 454–455:219–229PubMedCrossRefGoogle Scholar
- Baker AJM, Brooks RR (1989) Terrestrial higher plants which hyperaccumulate metallic elements - a review of their distribution, ecology and phytochemistry. Biorecovery 1:81–126Google Scholar
- Bedini S, Pellegrino E, Avio L, Pellegrini S, Bazzoffi E, Argese E, Giovannetti M (2009) Changes in soil aggregation and glomalin-related soil protein content as affected by the arbuscular mycorrhizal fungal species Glomus mosseae and Glomus intraradices. Soil Biol Biochem 41:1491–1496CrossRefGoogle Scholar
- De Jong L, Moreau X, Bestel I, Beaudoin E, Aimé A, Dolain C, Gladys Saez G, Tonetto A, Barthélémy P, Thiéry A (2013) Uptake of quantum dots into a freshwater flatworm : intracellular accumulation and transmission from parents to offspring. J Nanosci Lett 3:28Google Scholar
- ISO 10390 (2005) Soil quality—determination of pH. French version EN ISO 10390, AFNOR ParisGoogle Scholar
- ISO 10694 (1995) Soil quality—determination of organic and total carbon after dry combustion (elementary analysis). French version EN ISO 10694, AFNOR ParisGoogle Scholar
- ISO 11261 (1995) Soil quality—determination of total nitrogen—modified Kjeldhahl method. French version EN ISO 11261, AFNOR ParisGoogle Scholar
- ISO 11263 (1994) Soil quality—determination of phosphorus—spectrometric determination of phosphorus soluble in sodium hydrogen carbonate solution. French version EN ISO 11263, AFNOR ParisGoogle Scholar
- ISO 22036 (2008) Soil quality—determination of trace elements in extracts of soil by inductively coupled plasma-atomic emission spectrometry (ICP-AES). French version EN ISO 22036, AFNOR ParisGoogle Scholar
- Kabata-Pendias A (2004) Soil–plant transfer of trace elements—an environmental issue. Biogeochem Process Role Heavy Met Soil Environ 122:143–149Google Scholar
- Laffont-Schwob I, Dumas PJ, Pricop A, Rabier J, Miché L, Affre L, Masotti V, Prudent P, Tatoni T (2011) Insights on metal-tolerance and symbionts of the rare species Astragalus tragacantha aiming at phytostabilization of polluted soils and plant conservation. Ecol Mediterr Rev Int Ecol Méditerr Int J Mediterr Ecol 37:57–62Google Scholar
- Michalak A (2006) Phenolic compounds and their antioxidant activity in plants growing under heavy metal stress. Pol J Environ Stud 15:523–530Google Scholar
- Morel JL (1997) Assessment of phytoavailability of trace elements in soils. Analusis 25:M70–M72Google Scholar
- Pawlak-Sprada S, Stobiecki M, Deckert J (2011) Activation of phenylpropanoid pathway in legume plants exposed to heavy metals. Part II. Profiling of isoflavonoids and their glycoconjugates induced in roots of lupine (Lupinus luteus) seedlings treated with cadmium and lead. Acta Biochim Pol 58:217–223PubMedGoogle Scholar
- Rabie GH (2005) Contribution of arbuscular mycorrhizal fungus to red kidney and wheat plants tolerance grown in heavy metal-polluted soil. Afr J Biotechnol 4:332–345Google Scholar
- Raveux O (2002) L’usine à plomb de l’Escalette: le dernier grand vestige de la métallurgie marseillaise du plomb. Industries en Provence 10:7–9Google Scholar
- Testiati E, Parinet J, Massiani C, Laffont-Schwob I, Rabier J, Pfeifer HR, Lenoble V, Masotti V, Prudent P (2013) Trace metal and metalloid contamination levels in soils and in two native plant species of a former industrial site: evaluation of the phytostabilization potential. J Hazard Mater 248–249:131–141PubMedCrossRefGoogle Scholar
- Wuana RA, Okieimen FE (2011) Heavy metals in contaminated soils: a review of sources, chemistry, risks and best available strategies for remediation. Int Sch Res Netw Ecol 2011:1–20Google Scholar