Plant and Soil

, Volume 379, Issue 1–2, pp 261–274 | Cite as

The 4-phosphopantetheinyl transferase of Trichoderma virens plays a role in plant protection against Botrytis cinerea through volatile organic compound emission

  • Hexon Angel Contreras-Cornejo
  • Lourdes Macías-Rodríguez
  • Alfredo Herrera-Estrella
  • José López-Bucio
Regular Article

Abstract

Aims

This work was conducted to examine the effects of volatile organic compounds (VOCs) from Trichoderma virens and the 4-phosphopantetheinyl transferase 1 (TvPPT1) mutant in growth promotion and induction of defense responses of Arabidopsis thaliana seedlings using a co-cultivation system in vitro.

Methods

The contribution of VOCs to plant development and immunity was assessed by comparing the effectiveness of WT and Δppt1 mutant strains of T. virens in the formation of lateral roots and protection conferred against Botrytis cinerea. VOCs released by T. virens and Δppt1 mutant were compared by gas chromatography–mass spectrometry.

Results

Plants exposed to volatiles from WT T. virens showed 2-fold increase in fresh weight when compared to axenically-grown seedlings, which correlated with increased root branching and enhanced expression of the jasmonic acid-responsive marker pLox2:uidA as well as accumulation of jasmonic acid and hydrogen peroxide. T. virens produced a series of hydrocarbon terpenes, including the sesquiterpenes β-caryophyllene, (−)-β-elemene, germacrene D, τ-cadinene, δ-cadinene, α-amorphene, and τ-selinene and the monoterpenes β-myrcene, trans-β-ocimene, and cis-β-ocimene that were absent in TvPPT1 mutant.

Conclusions

Our results indicate that T. virens VOCs elicit both development and defense programs and that PPT1 plays an important role in biosynthesis of terpenes and plant protection against B. cinerea.

Keywords

Trichoderma Arabidopsis Root development Plant immunity Volatiles 

Abbreviations

FPP

Farnesyl pyrophosphate

GC-SIM-MS

Gas chromatography-selected ion monitoring mass spectrometry

JA

Jasmonic acid

MVA

Mevalonic acid

MEP

2-C-methyl-D-erythritol 4-phosphate

NRPS

Nonribosomal peptide synthase

OA

Orto-anisic acid

PKS

Poliketide synthase

POD

Peroxidase

PPT1

4-phosphopantetheinyl transferase 1

ROS

Reactive oxygen species

SA

Salicylic acid

VOCs

Volatile organic compounds

Notes

Acknowledgments

This work was supported by grants from the Consejo Nacional de Ciencia y Tecnología (CONACYT, México, grants no. 165738 and 177775), the Consejo de la Investigación Científica (UMSNH, México, grants no. CIC 2.24 and 2.26) and the Marcos Moshinsky Foundation. HACC is indebted to CONACYT for a doctoral fellowship.

Supplementary material

11104_2014_2069_MOESM1_ESM.docx (16 kb)
Supplementary Table 1(DOCX 16 kb)
11104_2014_2069_MOESM2_ESM.doc (60 kb)
Supplementary Table 2(DOC 60 kb)
11104_2014_2069_MOESM3_ESM.jpg (4.7 mb)
Supplementary Fig. S1Effect of T. virens WT and Δppt1-1 VOCs on induction of plant immunity. (a-h) Analysis of expression of the JA responsive gene marker pLox2:uidA and the SA responsive gene marker pPr-1a:uidA. GUS expression in Arabidopsis seedlings was determined after 5-days of co-cultivation with T. virens. Photographs show representative individuals of at least 10 stained seedlings. The experiment was repeated three times with similar results. (JPEG 4824 kb)

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Copyright information

© Springer International Publishing Switzerland 2014

Authors and Affiliations

  • Hexon Angel Contreras-Cornejo
    • 1
  • Lourdes Macías-Rodríguez
    • 1
  • Alfredo Herrera-Estrella
    • 2
  • José López-Bucio
    • 1
  1. 1.Instituto de Investigaciones Químico-BiológicasUniversidad Michoacana de San Nicolás de HidalgoMoreliaMexico
  2. 2.Laboratorio Nacional de Genómica para la Biodiversidad, CINVESTAV IrapuatoIrapuatoMexico

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