Plant and Soil

, Volume 377, Issue 1–2, pp 407–422 | Cite as

Mechanisms of trichome-specific Mn accumulation and toxicity in the Ni hyperaccumulator Alyssum murale

Regular Article

Abstract

Background and aims

Mechanisms of Mn accumulation and toxicity in and around trichomes on the Ni hyperaccumulator Alyssum murale were investigated.

Methods

Plants were grown aeroponically with variable amounts of Mn and Ni. Total metals were determined and electron microprobe analysis (EMPA) and synchrotron-based micro x-ray fluorescence (μ-SXRF) spectroscopy were used to evaluate metal distribution. Synchrotron techniques (μ-XANES, μ-EXAFS) along with infrared spectroscopy (DRIFT) were used to determine Mn speciation.

Results

At lower Mn concentrations or when grown together with Ni, Mn is confined to the trichome basal compartment in the +2 oxidation state in a complex with phosphate. At tissue concentrations >1,150 μg g−1 Mn-rich lesions develop around some trichomes in which greater amounts of Mn 3+ is found.

Conclusions

Mn is preferentially stored in trichomes on the plant surface which at higher concentrations enters the cell wall or apoplastic space of neighboring cells resulting in the formation of brown reaction products and oxidized Mn species. We propose a mechanism by which lesion formation and oxidized Mn species around some trichomes is possibly due to induction of the peroxidase system by excess Mn, triggering the accumulation of toxic phenoxy radicals and Mn3+.

Keywords

Manganese (Mn) Alyssum murale Trichome Hyperaccumulation Nickel (Ni) Synchrotron x-ray absorption fine structure spectroscopy (XAFS) 

Supplementary material

11104_2013_2003_MOESM1_ESM.docx (171 kb)
ESM 1(DOCX 170 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  1. 1.Rhizosphere Science Laboratory, Department of Plant and Soil Sciences, N122S Agricultural Sciences North BuildingUniversity of KentuckyLexingtonUSA

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