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Plant and Soil

, Volume 377, Issue 1–2, pp 295–308 | Cite as

Early vertical distribution of roots and its association with drought tolerance in tropical maize

  • C. Grieder
  • S. Trachsel
  • A. Hund
Regular Article

Abstract

Background and aims

Selection for deep roots to improve drought tolerance of maize (Zea mays L.) requires presence of genetic variation and suitable screening methods.

Methods

We examined a diverse set of 33 tropical maize inbred lines that were grown in growth columns in the greenhouse up to the 2-, 4-, and 6-leaf stage and in the field in Mexico. To determine length of roots from different depths at high throughput, we tested an approach based on staining roots with methylene blue and measuring the amount of absorbed dye as proxy measure for root length.

Results

Staining provided no advantage over root weights that are much easier to measure and therefore preferable. We found significant genotypic variation for all traits at the 6-leaf stage. For development rates between the 2-leaf and the 6-leaf stage, genotypes only differed for rooting depth and the number of crown roots. Positive correlations of leaf area with root length and rooting depth indicated a common effect of plant vigor. However, leaf area in growth columns was negatively related to grain yield under drought (r = −0.50).

Conclusion

The selection for deeper roots by an increase in plant vigor likely results in a poorer performance under drought conditions. The proportion of deep roots was independent of other traits but showed a low heritability and was not correlated to field performance. An improved screening protocol is proposed to increase throughput and heritability for this trait.

Keywords

Tropical maize Rooting depth Growth column Shoot-root relations 

Notes

Acknowledgments

The authors would like to thank Claude Welcker from INRA Montpellier for provision of seeds for growth column trials, Jill Cairns for overseeing trials hosted by CIMMYT as well as Vanessa Weber for her technical assistance during data collection and Ciro Sanchez for data collection and management of drought stress trials in the field. This study was supported by the Generation Challenge Programme (Project 15).

Supplementary material

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  1. 1.Institute of Agricultural SciencesETH ZurichZurichSwitzerland
  2. 2.International Maize and Wheat Improvement Center CIMMYTMexicoMexico

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