Plant and Soil

, Volume 371, Issue 1–2, pp 397–408 | Cite as

Assessment of the relationship between geologic origin of soil, rhizobacterial community composition and soil receptivity to tobacco black root rot in Savoie region (France)

  • Juliana Almario
  • Martina Kyselková
  • Jan Kopecký
  • Markéta Ságová-Marečková
  • Daniel Muller
  • Geneviève L. Grundmann
  • Yvan Moënne-Loccoz
Regular Article

Abstract

Background and aims

In Morens (Switzerland), soils formed on morainic deposits (which contain vermiculite clay and display particular tobacco rhizobacterial community) are naturally suppressive to Thielaviopsis basicola-mediated tobacco black root rot, but this paradigm was never assessed elsewhere. Here, we tested the relation between geology and disease suppressiveness in neighboring Savoie (France).

Methods

Two morainic and two sandstone soils from Savoie were compared based on disease receptivity (T. basicola inoculation tests on tobacco), clay mineralogy (X-ray diffraction), tobacco rhizobacterial community composition (16S rRNA gene-based taxonomic microarray) and phlD+Pseudomonas populations involved in 2,4-diacetylphloroglucinol production (real-time PCR and tRFLP).

Results

Unlike in Morens, in Savoie the morainic soils were receptive to disease whereas T. basicola inoculation did not increase disease level in the sandstone soils. Vermiculite was not present in Savoie soils. The difference in rhizobacterial community composition between Savoie morainic and sandstone soils was significant but modest, and there was little agreement in bacterial taxa discriminating soils of different disease receptivity levels when comparing Morens versus Savoie soils. Finally, phlD+ rhizosphere pseudomonads were present at levels comparable to those in Morens soils, but with different diversity patterns.

Conclusions

The morainic model of black root rot suppressiveness might be restricted to the particular type of moraine occurring in the Morens region, and the low disease receptivity of sandstone soils in neighboring Savoie might be related to other plant-protection mechanisms.

Keywords

Suppressive soil Thielaviopsis basicola Black root rot Moraine Rhizosphere Bacterial community 16S microarray real-time PCR 2,4-diacetylphloroglucinol 

Supplementary material

11104_2013_1677_MOESM1_ESM.ppt (4.8 mb)
Fig. S1Geographic locations of Seyssel and Albens in Savoie, compared with that of Morens (Switzerland) (A), and location on IGN/BRGM geological maps (http://www.geoportail.gouv.fr/accueil) of the Savoie soils Ymo4 (moraine) and Ysa5 (sandstone) near Seyssel (B), and Amo1 (moraine) and Asa2 (sandstone) near Albens (C). (PPT 4.79 MB)
11104_2013_1677_MOESM2_ESM.ppt (176 kb)
Fig. S2Principal component analysis (PCA) of rhizobacterial communities from Thielaviopsis basicola-inoculated (full symbols) and non-inoculated (empty symbols) samples, based on microarray probe signals. Data are shown as means and standard errors of sample positions. (A) Comparison of Savoie sandstone (Asa2 and Ysa5) and morainic (Amo1 and Ymo4) soils. Principal components PC1 and PC2 corresponded to respectively 29 % and 13 % data variation. The treatments could not be statistically distinguished in the PCA ordination plot. (B) Comparison of Savoie sandstone (Asa2 and Ysa5) and morainic (Amo1 and Ymo4) soils, along with data obtained previously under the same conditions for two Morens soils (i.e. suppressive morainic soil MS8 and conducive sandstone soil MC112; Kyselková et al. 2009), which were cropped with wheat (MS8) and mixed pasture (MC112) at the time of sampling. Principal components PC1 and PC2 corresponded to respectively 30 % and 10 % data variation. Differences between treatments are shown using letters AB along axis PCA1 and a-d along axis PCA2 (ANOVA and Fisher LSD tests; P < 0.05) (PPT 172 kb)
11104_2013_1677_MOESM3_ESM.pdf (40 kb)
ESM 3(PDF 40 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Juliana Almario
    • 1
    • 2
    • 3
  • Martina Kyselková
    • 1
    • 2
    • 3
    • 4
  • Jan Kopecký
    • 5
  • Markéta Ságová-Marečková
    • 5
  • Daniel Muller
    • 1
    • 2
    • 3
  • Geneviève L. Grundmann
    • 1
    • 2
    • 3
  • Yvan Moënne-Loccoz
    • 1
    • 2
    • 3
  1. 1.Université de LyonLyonFrance
  2. 2.Université Lyon 1VilleurbanneFrance
  3. 3.CNRS, UMR5557, Ecologie MicrobienneVilleurbanneFrance
  4. 4.Biology Centre of the Academy of Sciences of the Czech RepublicInstitute of Soil BiologyČeské BudějoviceCzech Republic
  5. 5.Department of Plant PathologyCrop Research InstitutePraha-RuzyněCzech Republic

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