Establishment and survival of the South African legume Lessertia spp. and rhizobia in Western Australian agricultural systems
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Background and aims
The South African herbaceous legume species Lessertia capitata, L. diffusa, L. excisa L. incana and L. herbacea were introduced to Australia to assess plant establishment and survival, as well as the saprophytic ability of their root nodule bacteria (RNB).
Five Lessertia spp., were inoculated with selected RNB strains and were sown in five different agroclimatic areas of the Western Australian wheat-belt during 2007 and 2008. Plant population and summer survival were evaluated in situ. Soil samples and nodules from host plants were also taken from each site. The re-isolated rhizobia were RPO1-PCR fingerprinted and their partial dnaK and nodA genes were sequenced to confirm their identity.
Plants achieved only poor establishment followed by weak summer survival. More than 83 % of the rhizobia re-isolated from Lessertia did not correlate with the original inoculants’ fingerprints, and were identified as Rhizobium leguminosarum. The nodA sequences of the naturalised strains were also clustered with R. leguminosarum sequences, thus eliminating the likelihood of lateral gene transference from Mesorhizobium and suggesting a competition problem with indigenous rhizobia.
The stressful soil conditions and high numbers of resident R. leguminosarum strains in Western Australian soils, and their ability to rapidly nodulate Lessertia spp. but not fix nitrogen are likely to preclude the adoption of Lessertia as an agricultural legume in this region.
KeywordsMesorhizobium Lessertia spp. Nodulation Selection Symbiotic nitrogen fixation
Western soil microbiology
- ½ LA
Half Lupin Agar
The authors would like to thank Mrs. Regina Carr (Centre for Rhizobium studies, Murdoch University) for technical assistance.
- Allen ON, Allen EK (1981) The Leguminosae: a source book of characteristics, uses and nodulation. The University of Wisconsin Press, Wisconsin, p 812Google Scholar
- Balkwill MJ, Balkwill K (1999) The genus Lessertia DC. (Fabaceae-Galegeae) in KwaZulu-Natal (South Africa). S Afr J Bot 65:339–356Google Scholar
- Breebaart L (2003) Feeding selection in three grazing systems in the Nama Karoo. In: Conservation farming project. National Botanical Institute, South AfricaGoogle Scholar
- Graham PH (2008) Ecology of the root-nodule bacteria of legumes. In: Dilworth MJ, James EK, Sprent JI, Newton WE (eds) Nitrogen-fixing Leguminous Symbioses. Springer, Dordrecht, pp 23–58Google Scholar
- Harvey WH (1862) Leguminosae. In: Harvey WH, Sonder OW (eds) Flora Capensis: systematic description of the plants of the Cape Colony, Caffraria and Port Natal. Vol. 2. Hodges, Smith and Co, Dublin, pp 1–285Google Scholar
- Herridge D (2008) Inoculation technology for legumes. In: Dilworth MJ, James EK, Sprent JI, Newton WE (eds) Nitrogen-fixing Leguminous Symbioses. Springer, Dordrecht, pp 77–115Google Scholar
- Hoffmann T (1996a) Nama Karoo biome. In: Low AB, Robelo AG (eds) Vegetation of South Africa, Lesotho and Swaziland. Department of Environmental Affairs and Tourism, PretoriaGoogle Scholar
- Hoffmann T (1996b) Succulent Karoo biome. In: Low AB, Robelo AG (eds) Vegetation of South Africa, Lesotho and Swaziland. Department of Environmental Affairs and Tourism, PretoriaGoogle Scholar
- Howieson JG, Yates RJ, Foster KJ, Real D, Besier RB (2008) Prospects for the future use of legumes. In: Dilworth MJ, James EK, Sprent JI, Newton WE (eds) Nitrogen-fixing Leguminous Symbioses. Springer, Dordrecht, pp 363–393Google Scholar
- Lock JM, Schrire BD (2005) Tribe Galegae. In: Lewis G, Schrire BD, Mackinder B, Lock JM (eds) Legumes of the world. Royal Botanic Gardens, Kew, pp 475–487Google Scholar
- López-Lara IM, Blok-Tip L, Quinto C, Garcia M, Stacey G, Bloemberg GV, Lamers GEM, Lugtenberg BJJ, Thomas-Oates JE, Spaink HP (1996) NodZ of Bradyrhizobium extends the nodulation host range of Rhizobium by adding a fucosyl residue to nodulation signals. Mol Microbiol 21:397–408PubMedCrossRefGoogle Scholar
- McKenzie N, Jacquier D, Isbell R, Brown K (2004) Australian soils and landscapes: an illustrated compendium. CSIRO publishingGoogle Scholar
- Nkonki T (2004) Lessertia DC. National Herbarium, Pretoria. http://www.plantzafrica.com/plantklm/lessertia.htm. Accessed 04 January 2010
- O'Hara GW, Yates RJ, Howieson JG (2002) Selection of strains of root nodule bacteria to improve inoculant performance and increase legume productivity in stressful environments. In: Herridge D (ed) Inoculants and nitrogen fixation of legumes in Vietnam. ACIAR Proceedings, pp 72–80Google Scholar
- Robelo AG (1996) Fynbos biome. In: Low AB, Robelo AG (eds) Vegetation of South Africa, Lesotho and Swaziland. Department of Environmental Affairs and Tourism, PretoriaGoogle Scholar
- Sanderson MJ, Liston A (1995) Molecular phylogenetic sistematics of Galegeae, with special reference to Astragalus. In: Crisp MD, Doyle JJ (eds) Advances in Legume systematics. Part 7: phylogeny. The Royal Botanic Gardens, Kew, pp 331–344Google Scholar
- Vekemans X (2002) AFLP-SURV version 1.0. Distributed by the author. Laboratoire de Genétique et Ecologie Vegétale, Université Libre de Bruxelles, BelgiumGoogle Scholar
- Yates RJ, Howieson JG, Reeve WG, Nandasena KG, Law IJ, Bräu L, Ardley JK, Nistelberger HM, Real D, O'Hara GW (2007) Lotononis angolensis forms nitrogen fixing, lupinoid nodules with phylogenetically unique, fast-growing, pink-pigmented bacteria, which do not nodulate L. bainesii or L. listii. Soil Biol Biochem 39:1680–1688CrossRefGoogle Scholar